(270) AMPK-mediated control of P bodies as a novel mechanism of gene expression control in peripheral sensory neurons

Pat1 proteins are conserved across eukaryotes. Vertebrates have evolved two Pat1 proteins paralogues, whereas invertebrates and yeast only possess one such protein. Despite their lack of known domains or motifs, Pat1 proteins are involved in several key post-transcriptional mechanisms of gene expression control. In yeast, Pat1p interacts with translating mRNPs (messenger ribonucleoproteins), and is responsible for translational repression and decapping activation, ultimately leading to mRNP degradation. Drosophila HPat and human Pat1b (PatL1) proteins also have conserved roles in the 5′→3′ mRNA decay pathway. Consistent with their functions in silencing gene expression, Pat1 proteins localize to P-bodies (processing bodies) in yeast, Drosophila, Caenorhabditis elegans and human cells. Altogether, Pat1 proteins may act as scaffold proteins allowing the sequential binding of repression and decay factors on mRNPs, eventually leading to their degradation. In the present mini-review, we present the current knowledge on Pat1 proteins in the context of their multiple functions in post-transcriptional control.

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Faculty Opinions recommendation of Global quantification of mammalian gene expression control.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.10925958.12978054 ◽

2011 ◽

Author(s):

Jürg Bahler

Keyword(s):

Gene Expression ◽

Mammalian Gene ◽

Expression Control ◽

Gene Expression Control ◽

Mammalian Gene Expression

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Faculty Opinions recommendation of Global quantification of mammalian gene expression control.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.10925958.12716054 ◽

2011 ◽

Author(s):

Luc DesGroseillers ◽

Karine Boulay

Keyword(s):

Gene Expression ◽

Mammalian Gene ◽

Expression Control ◽

Gene Expression Control ◽

Mammalian Gene Expression

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Correction to “Regulation of d Opioid Receptor-Mediated Signaling and Antinociception in Peripheral Sensory Neurons by Arachidonic Acid–Dependent 12/15-Lipoxygenase Metabolites”

Journal of Pharmacology and Experimental Therapeutics ◽

10.1124/jpet.112.241604err ◽

2019 ◽

Vol 369 (1) ◽

pp. 143-143

Keyword(s):

Arachidonic Acid ◽

Opioid Receptor ◽

Sensory Neurons ◽

Peripheral Sensory Neurons ◽

Peripheral Sensory

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High-performance chemical- and light-inducible recombinases in mammalian cells and mice

Nature Communications ◽

10.1038/s41467-019-12800-7 ◽

2019 ◽

Vol 10 (1) ◽

Cited By ~ 9

Author(s):

Benjamin H. Weinberg ◽

Jang Hwan Cho ◽

Yash Agarwal ◽

N. T. Hang Pham ◽

Leidy D. Caraballo ◽

...

Keyword(s):

Gene Expression ◽

Mammalian Cells ◽

High Performance ◽

Genome Engineering ◽

Genetic Circuits ◽

Control Of Gene Expression ◽

Expression Control ◽

Gene Expression Control ◽

High Performance Systems ◽

Spatiotemporal Control

Abstract Site-specific DNA recombinases are important genome engineering tools. Chemical- and light-inducible recombinases, in particular, enable spatiotemporal control of gene expression. However, inducible recombinases are scarce due to the challenge of engineering high performance systems, thus constraining the sophistication of genetic circuits and animal models that can be created. Here we present a library of >20 orthogonal inducible split recombinases that can be activated by small molecules, light and temperature in mammalian cells and mice. Furthermore, we engineer inducible split Cre systems with better performance than existing systems. Using our orthogonal inducible recombinases, we create a genetic switchboard that can independently regulate the expression of 3 different cytokines in the same cell, a tripartite inducible Flp, and a 4-input AND gate. We quantitatively characterize the inducible recombinases for benchmarking their performances, including computation of distinguishability of outputs. This library expands capabilities for multiplexed mammalian gene expression control.

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