Simultaneous analysis of eight bioactive compounds in Danning tablet by HPLC-ESI-MS and HPLC-UV

Abstract Background Ocimum genus known as Tulsi or Basil is a prominent botanical class in Asian culture, especially in India. The leaves have an immunomodulatory, antioxidant, stress-relieving, and adaptogenic role in traditional and modern medicine, with prominent usage in herbal teas and nutraceuticals. Objective The HPLC-PDA method was developed and validated for vicenin-2, orientin, cynaroside, betulinic acid, genistein, with syringic acid, rosmarinic acid, eugenol, carnosic acid, oleanolic acid, ursolic acid, luteolin, apigenin for quantification and confirmed using a novel ESI -MS/MS method in the Ocimum samples. Method The methodology parameters were developed on the RP-C18 column with a gradient elution of 1 mL/min flow rate for 0.1% o-phosphoric acid and acetonitrile at 210 and 340 nm wavelengths. Result The validation data for 13 bioactive compounds showed good linearity (r2> 0.99) with sensitive LOD (0.034-0.684 µg/mL) and LOQ (0.100-2.068 µg/mL) with recoveries (83.66-101.53%).The results were found precise (RSD,<5.0%) and accurate (RE, -0.60-1.06) for the quantification. The method performance was verified and found robust by analyzing ten samples of O. tenuiflorum from the ten geographical states of India (RSD, < 5.0%). Conclusion The validated HPLC-PDA method was found selective and suitable for analyzing thirteen compounds in O. tenuiflorum and twelve cultivars from the Ocimum genus as a quality control tool. This method can be used in routine analysis as an inexpensive alternative to advanced techniques. Highlights This work is the first to report for vicenin-2, orientin, cynaroside, betulinic acid, and genistein, with simultaneous analysis of eight bioactive compounds in the Ocimum genus.

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Simultaneous analysis of 11 main active components in Cirsium setosum based on HPLC-ESI-MS/MS and combined with statistical methods

Journal of Separation Science ◽

10.1002/jssc.201200359 ◽

2012 ◽

Vol 35 (21) ◽

pp. 2897-2907 ◽

Cited By ~ 13

Author(s):

Qian Sun ◽

Lu Chang ◽

Yanping Ren ◽

Liang Cao ◽

Yingguang Sun ◽

...

Keyword(s):

Statistical Methods ◽

Simultaneous Analysis ◽

Active Components ◽

Esi Ms

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LC–ESI–MS/MS characterization, antioxidant power and inhibitory effects on α-amylase and tyrosinase of bioactive compounds from hulls of Amygdalus communis: The influence of the extracting solvents

Industrial Crops and Products ◽

10.1016/j.indcrop.2018.11.014 ◽

2019 ◽

Vol 128 ◽

pp. 147-152 ◽

Cited By ~ 22

Author(s):

Nizar Tlili ◽

Bulent Kirkan ◽

Cengiz Sarikurkcu

Keyword(s):

Bioactive Compounds ◽

Inhibitory Effects ◽

Antioxidant Power ◽

Esi Ms ◽

Amygdalus Communis

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Bioactive Compounds in Sarcocornia and Arthrocnemum, Two Wild Halophilic Genera from the Iberian Peninsula

Plants ◽

10.3390/plants10102218 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2218

Author(s):

Irene Sánchez-Gavilán ◽

Esteban Ramírez Chueca ◽

Vicenta de la Fuente García

Keyword(s):

Fatty Acids ◽

Salt Tolerance ◽

Ferulic Acid ◽

Iberian Peninsula ◽

Bioactive Compounds ◽

Phenolic Acids ◽

Coastal Marshes ◽

Esi Ms ◽

Tinto River ◽

High Diversity

(1) Background: this study describes bioactive compounds in the following halophytes: Sarcocornia (S. alpini, S. pruinosa, and S. perennis) and Arthrocnemum (A. macrostachyum). The material comes from: coastal marshes in Tinto River, Guadiana River, and some interior provinces from the Iberian Peninsula. (2) Methods: the techniques used were Folin–Ciocalteu, GC-MS, and ESI-MS/MS. (3) Results: Five phenolic acids were found in Sarcocornia: trans-cinnamic, salicylic, veratric, coumaric, and caffeic acids. In addition, in Arthronemum, ferulic acid was also detected. The obtained flavonoids were cyanidin-3-O-arabinoside, luteolin-7-glucoside, dihydroquercetin, and p-coumaroyl-glucoside. They also presented fatty acids, such as palmitic, linoleic, and oleic acids in Sarcocornia, while palmitic, linolenic, and stearic acids were the main fatty acids in A. macrostachyum. (4) Conclusions: the high diversity of the compounds identified confirms the relation between nutritional interest and salt tolerance in halophytes.

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Array of Metabolites in Italian Hericium erinaceus Mycelium, Primordium, and Sporophore

Molecules ◽

10.3390/molecules24193511 ◽

2019 ◽

Vol 24 (19) ◽

pp. 3511 ◽

Cited By ~ 6

Author(s):

Federica Corana ◽

Valentina Cesaroni ◽

Barbara Mannucci ◽

Rebecca Michela Baiguera ◽

Anna Maria Picco ◽

...

Keyword(s):

Dietary Supplements ◽

Bioactive Compounds ◽

Medicinal Mushroom ◽

Wild Type ◽

Hericium Erinaceus ◽

Esi Ms ◽

Therapeutic Properties ◽

Erinacine A ◽

First Time

Hericium erinaceus is a medicinal mushroom that contains many molecules promising a plethora of therapeutic properties. In this study, the strain H.e.2 (MicUNIPV, University of Pavia, Italy) was isolated from a sporophore collected in Tuscany (Italy). Mycelium, primordium, and wild type and cultivated sporophores were analyzed by HPLC-UV-ESI/MS. Erinacine A in the mycelium and hericenones C and D in the sporophores were quantified by comparison with their standard molecules. For the first time, H. erinaceus primordium was also investigated for the presence of these molecules. Comparing with the literature data, hericenes, molecules structurally similar to hericenones, were present in all our samples. The highest contents of hericenones C and D were detected in cultivated sporophores, compared to the wild type. The comparison of these data with those of another Italian H. erinaceus strain (H.e.1 MicUNIPV) was discussed. The results led us to select H. erinaceus strains more suitable for mycelium production or sporophore cultivation to obtain extracts with a higher content of bioactive compounds. This work provides a further step towards standardizing the procedures in the development of dietary supplements made from mushrooms.

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