Quantification of psychosine in the serum of twitcher mouse by LC–ESI-tandem-MS analysis

2013 ◽  
Vol 80 ◽  
pp. 44-49 ◽  
Author(s):  
Assunta Zanfini ◽  
Elena Dreassi ◽  
Anna Berardi ◽  
Laura Governini ◽  
Gianfranco Corbini ◽  
...  
Keyword(s):  
Tandem Ms ◽  
Twitcher Mouse ◽  
Ms Analysis

scholarly journals Tandem MS Analysis of Selenamide-Derivatized Peptide Ions

2011 ◽  
Vol 22 (9) ◽  
pp. 1610-1621 ◽  
Author(s):  
Yun Zhang ◽  
Hao Zhang ◽  
Weidong Cui ◽  
Hao Chen
Keyword(s):  
Tandem Ms ◽  
Peptide Ions ◽  
Ms Analysis

Strategy for the enrichment of protein biomarkers from diverse bacterial select agents

2021 ◽  
Vol 28 ◽  
Author(s):  
Sasikumar Sabna ◽  
Dev Vrat Kamboj ◽  
Ravi Bhushan Kumar ◽  
Prabhakar Babele ◽  
Sakshi Rajoria ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Species Identification ◽  
Immune Serum ◽  
Tandem Ms ◽  
Environmental Matrices ◽  
Bacterial Proteins ◽  
Conserved Regions ◽  
Groel Protein ◽  
Ms Analysis ◽  
Enrichment Strategy

Background: Some pathogenic bacteria can be potentially used for nefarious applications in the event of bioterrorism or biowarfare. Accurate identification of biological agent from clinical and diverse environmental matrices is of paramount importance for implementation of medical countermeasures and biothreat mitigation. Objective: A novel methodology is reported here for the development of a novel enrichment strategy for the generally conserved abundant bacterial proteins for an accurate downstream species identification using tandem MS analysis in biothreat scenario. Methods: Conserved regions in the common bacterial protein markers were analyzed using bioinformatic tools and stitched for a possible generic immuno-capture for an intended downstream MS/MS analysis. Phylogenetic analysis of selected proteins was carried out and synthetic constructs were generated for the expression of conserved stitched regions of 60 kDa chaperonin GroEL. Hyper-immune serum was raised against recombinant synthetic GroEL protein. Results: The conserved regions of common bacterial proteins were stitched for a possible generic immuno-capture and subsequent specific identification by tandem MS using variable regions of the molecule. Phylogenetic analysis of selected proteins was carried out and synthetic constructs were generated for the expression of conserved stitched regions of GroEL. In a proof-of-concept study, hyper-immune serum raised against recombinant synthetic GroEL protein exhibited reactivity with ~60 KDa proteins from the cell lysates of three bacterial species tested. Conclusion: The envisaged methodology can lead to the development of a novel enrichment strategy for the abundant bacterial proteins from complex environmental matrices for the downstream species identification with increased sensitivity and substantially reduce the time-to-result.

Characterization of major degradation products of an adenosine A2Areceptor antagonist under stressed conditions by LC-MS and FT tandem MS analysis

2010 ◽  
Vol 45 (2) ◽  
pp. 146-156 ◽  
Author(s):  
Li-Kang Zhang ◽  
Birendra N. Pramanik
Keyword(s):  
Degradation Products ◽  
Tandem Ms ◽  
Ms Analysis

scholarly journals Tryptophan glycoconjugates in food and human urine*

1999 ◽  
Vol 343 (1) ◽  
pp. 11-19 ◽  
Author(s):  
Birgit GUTSCHE ◽  
Christoph GRUN ◽  
Dieter SCHEUTZOW ◽  
Markus HERDERICH
Keyword(s):  
Human Urine ◽  
Quantum Coherence ◽  
Nuclear Overhauser Effect ◽  
Multiple Bond ◽  
Spatial Arrangement ◽  
Coupling Constants ◽  
Tandem Ms ◽  
Multiple Quantum ◽  
Hydroxymethyl Group ◽  
Ms Analysis

Evaluating the formation of tryptophan glycoconjugates other than well-established Amadori rearrangement products, HPLC-tandem MS (MS/MS) analysis of human urine collected from several healthy individuals proved the presence of one distinct tryptophan C-glycosyl compound [Horiuchi, Yonekawa, Iwahara, Kanno, Kurihara and Fujise (1994) J. Biochem. (Tokyo) 115, 362-366]. After isolation, unambiguous identification of this novel tryptophan metabolite as 2-(α-mannopyranosyl)-L-tryptophan was achieved by tandem MS combined with NMR spectroscopy including homonuclear COSY, heteronuclear multiple-bond connectivity and 1H-detected heteronuclear multiple-quantum coherence experiments. Remarkably, a thorough evaluation of vicinal proton-proton coupling constants in different solvents and nuclear Overhauser effect experiments demonstrate the predominant axial orientation of the hydroxymethyl group of the hexopyranosyl residue. Likewise this spatial arrangement indicates that the respective α-anomeric C-mannosylhexopyranose is preferentially adopting a 1C4 conformation in acidic methanol. Whereas only one distinct tryptophan mannoconjugate could be observed in human urine, HPLC-MS/MS analysis of food samples for the first time led to the identification of numerous N1-(β-D-hexopyranosyl)-L-tryptophan, 2-(β-D-hexopyranosyl)-L-tryptophan and 1-(1,2,3,4,5-p e n t a h y d r o x y p e n t - 1 - yl) - 1, 2, 3, 4 - t e t r a h y d r o - β - c a r b o l i n e - 3 -carboxylic acid derivatives derived from the condensation of tryptophan with aldohexoses. Taking into consideration the significant differences between profiles and configurations of tryptophan glycoconjugates originating from dietary sources and human urine, C-2 mannosylation of tryptophan residues [de Beer, Vliegenthart, Loeffler and Hofsteenge (1995) Biochemistry 34, 11785-11789] represents a novel enzymic pathway in tryptophan metabolism in humans.

Molecular dissection of membrane-transport proteins: mass spectrometry and sequence determination of the galactose–H+ symport protein, GalP, of Escherichia coli and quantitative assay of the incorporation of [ring-2-13C]histidine and 15NH3

2002 ◽  
Vol 363 (2) ◽  
pp. 243-252 ◽  
Author(s):  
Henrietta VENTER ◽  
Alison E. ASHCROFT ◽  
Jeffrey N. KEEN ◽  
Peter J.F. HENDERSON ◽  
Richard B. HERBERT
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Membrane Transport ◽  
Dna Sequence ◽  
Transport Proteins ◽  
Tandem Ms ◽  
Esi Ms ◽  
Membrane Transport Proteins ◽  
Ms Analysis

The molecular mass of the galactose—H+ symport protein GalP, as its histidine-tagged derivative GalP(His)6, has been determined by electrospray MS (ESI-MS) with an error of <0.02%. One methionine residue, predicted to be present from the DNA sequence, was deduced to be absent. This is a significant advance on the estimation of the molecular masses of membrane-transport proteins by SDS/PAGE, where there is a consistent under-estimation of the true molecular mass due to anomalous electrophoretic migration. Addition of a size-exclusion chromatography step after Ni2+-nitrilotriacetate affinity purification was essential to obtain GalP(His)6 suitable for ESI-MS. Controlled trypsin, trypsin+chymotrypsin and CNBr digestion of the protein yielded peptide fragments suitable for ESI-MS and tandem MS analysis, and accurate mass determination of the derived fragments resulted in identification of 82% of the GalP(His)6 protein. Tandem MS analysis of selected peptides then afforded 49% of the actual amino acid sequence of the protein; the absence of the N-terminal methionine was confirmed. Matrix-assisted laser-desorption ionization MS allowed identification of one peptide that was not detected by ESI-MS. All the protein/peptide mass and sequence determinations were in accord with the predictions of amino acid sequence deduced from the DNA sequence of the galP gene. [ring-2-13C]Histidine was incorporated into GalP(His)6in vivo, and ESI-MS analysis enabled the measurement of a high (80%) and specific incorporation of label into the histidine residues in the protein. MS could also be used to confirm the labelling of the protein by 15NH3 (93% enrichment) and [19F]tryptophan (83% enrichment). Such MS measurements will serve in the future analysis of the structures of membrane-transport proteins by NMR, and of their topology by indirect techniques.

scholarly journals Tandem MS analysis of brain clathrin-coated vesicles reveals their critical involvement in synaptic vesicle recycling

2004 ◽  
Vol 101 (11) ◽  
pp. 3833-3838 ◽  
Author(s):  
F. Blondeau ◽  
B. Ritter ◽  
P. D. Allaire ◽  
S. Wasiak ◽  
M. Girard ◽  
...  
Keyword(s):  
Synaptic Vesicle ◽  
Coated Vesicles ◽  
Tandem Ms ◽  
Synaptic Vesicle Recycling ◽  
Vesicle Recycling ◽  
Ms Analysis

GC-EI-MS Analysis of Fatty Acid Composition in Brain and Serum of Twitcher Mouse

Lipids ◽  
2014 ◽  
Vol 49 (11) ◽  
pp. 1115-1125 ◽  
Author(s):  
Assunta Zanfini ◽  
Elena Dreassi ◽  
Anna Berardi ◽  
Paola Piomboni ◽  
Elvira Costantino-Ceccarini ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Twitcher Mouse ◽  
Ms Analysis
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