A novel hydrophilic interaction liquid chromatography method for the determination of underivatized amino acids in alimentary supplements

2017 ◽  
Vol 145 ◽  
pp. 751-757 ◽  
Author(s):  
Thomas Themelis ◽  
Roberto Gotti ◽  
Rita Gatti
Keyword(s):  
Amino Acids ◽  
Liquid Chromatography ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Hydrophilic Interaction ◽  
Chromatography Method ◽  
Liquid Chromatography Method ◽  
Underivatized Amino Acids

scholarly journals Development of Hydrophilic Interaction Liquid Chromatography Method for the Analysis of Moxonidine and Its Impurities

2016 ◽  
Vol 2016 ◽  
pp. 1-7
Author(s):  
Slavica Filipic ◽  
Milica Elek ◽  
Marija Popović ◽  
Katarina Nikolic ◽  
Danica Agbaba
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Hydrophilic Interaction ◽  
Chromatography Method ◽  
Pharmaceutical Dosage Form ◽  
Ich Guidelines ◽  
Liquid Chromatography Method

Fast and simple hydrophilic interaction liquid chromatography (HILIC) method was developed and validated for the analysis of moxonidine and its four impurities (A, B, C, and D) in pharmaceutical dosage form. All experiments were performed on the Agilent Technologies 1200 high-performance liquid chromatography (HPLC) system using Zorbax RX-SIL, 250 mm × 4.6 mm, 5 μm column as stationary phase (T=25°C, F=1 mL/min, and λ=255 nm), and mixture of acetonitrile and 40 mM ammonium formate buffer (pH 2.8) 80 : 20 (v/v) as mobile phase. Under the optimal chromatographic conditions, selected by central composite design, separation and analysis of moxonidine and its four impurities are enabled within 12 minutes. Validation of the method was conducted in accordance with ICH guidelines. Based on the obtained results selectivity, linearity (r≥0.9976), accuracy (recovery: 93.66%–114.08%), precision (RSD: 0.56%–2.55%), and robustness of the method were confirmed. The obtained values of the limit of detection and quantification revealed that the method can be used for determination of impurities levels below 0.1%. Validated method was applied for determination of moxonidine and its impurities in commercially available tablet formulation. Obtained results confirmed that validated method is fast, simple, and reliable for analysis of moxonidine and its impurities in tablets.

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