Quantification of methylisomiroestrol, a phytoestrogen of Pueraria candollei, by enzyme-linked immunosorbent assay in comparison with high-performance liquid chromatography

Abstract The aim of this study was to investigate the changes in circulating concentrations of citrulline, neopterin, kynurenine, and tryptophan during the course of chemoradiation in patients with cervical cancer. Sixteen patients with histologically confirmed carcinoma of the uterine cervix, aged 53 ± 15 years (range 29–76 years), were included in this study. Plasma neopterin, kynurenine, and tryptophan were determined with an enzyme-linked immunosorbent assay. Plasma citrulline was measured with high-performance liquid chromatography. Compared to baseline, citrulline concentration was markedly and statistically significantly decreased at visits 2, 3, and 4, while returning to pretreatment concentrations at visit 5. A significant increase in serum neopterin concentrations was observed at visits 4 and 5. With the exception of decreased kynurenine/tryptophan ratio at visit 3, no significant changes were observed in the concentrations of kynurenine, tryptophan, and kynurenine/tryptophan ratio throughout the course of the treatment. In conclusion, present data demonstrate that citrulline concentrations decrease early and neopterin concentrations increase late during the course of chemoradiation in patients with cervical carcinoma. Citrulline represents a biomarker of intestinal toxicity in this population.

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Comparison of enzyme-linked immunosorbent assay and high-performance liquid chromatography for the analysis of atrazine in water from Czechoslovakia

Archives of Environmental Contamination and Toxicology ◽

10.1007/bf01060357 ◽

1991 ◽

Vol 21 (3) ◽

pp. 365-370 ◽

Cited By ~ 33

Author(s):

Rodney J. Bushway ◽

Lewis B. Perkins ◽

Ladislav Fukal ◽

Robert O. Harrison ◽

Bruce S. Ferguson

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Immunosorbent Assay ◽

High Performance ◽

Enzyme Linked Immunosorbent Assay

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PERBANDINGAN BEBERAPA METODE ANALISIS HISTAMIN UNTUK PRODUK PERIKANAN

SQUALEN Bulletin of Marine and Fisheries Postharvest and Biotechnology ◽

10.15578/squalen.v4i2.150 ◽

2009 ◽

Vol 4 (2) ◽

pp. 48

Author(s):

Hedi Indra Januar

Keyword(s):

High Performance Liquid Chromatography ◽

Capillary Electrophoresis ◽

Liquid Chromatography ◽

Capillary Zone Electrophoresis ◽

Immunosorbent Assay ◽

High Performance ◽

Enzyme Linked Immunosorbent Assay ◽

Capillary Electrophoresis Capillary ◽

Zone Electrophoresis ◽

Capillary Zone

Studi ini bertujuan untuk membandingkan beberapa metode dalam penentuan kadar amina biogenik histamin. Diketahui, kadar histamin merupakan salah satu parameter yang penting sebagai standar kualitas produk perikanan. Metode yang dibandingkan meliputi metode menggunakan spektrofluorometri, High Performance Liquid Chromatography (HPLC), Enzyme Linked Immunosorbent Assay (ELISA), dan Capillary Electrophoresis/Capillary Zone Electrophoresis (CE/CZE). Hasil perbandingan menunjukkan bahwa baik dari sisi ketidakpastian yang mungkin ditimbulkan dari metodenya serta hasil uji kelayakan laboratorium di Eropa, metode HPLC derivatisasi post-kolom merupakan metode yang optimal saat ini untuk menentukan kadar histamin secara kuantitatif. Akan tetapi, untuk pertimbangan efisiensi waktu, maka studi ini mengusulkan bahwa penggabungan metode ELISA kualitatif dan HPLC kuantitatif sangat baik dijadikan sebagai standar metode penentuan histamin di laboratorium pengujian produk perikanan.

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