Evaluation of high performance liquid chromatography (HPLC), enzyme linked immunosorbent assay (ELISA) and particle concentration fluorescence immunoassay (PCFIA) methods for the screening, quantitation and pharmacokinetic study of furosemide in horses

1990 ◽  
Vol 47 (1) ◽  
pp. 1-15 ◽  
Author(s):  
A.K. Singh ◽  
C. McArdle ◽  
M. Ashraf ◽  
K. Granley ◽  
U. Mishra ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Immunosorbent Assay ◽  
Pharmacokinetic Study ◽  
High Performance ◽  
Particle Concentration ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fluorescence Immunoassay

scholarly journals Circulating concentrations of citrulline, neopterin, kynurenine, and tryptophan during chemoradiation in patients with cervical carcinoma

Pteridines ◽  
2021 ◽  
Vol 32 (1) ◽  
pp. 93-97
Author(s):  
Markéta Pospíšková ◽  
Ondřej Strouhal ◽  
Eva Hlídková ◽  
Zuzana Vlachová ◽  
Bohuslav Melichar ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Cervical Carcinoma ◽  
Immunosorbent Assay ◽  
Uterine Cervix ◽  
High Performance ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Neopterin ◽  
Plasma Citrulline

Abstract The aim of this study was to investigate the changes in circulating concentrations of citrulline, neopterin, kynurenine, and tryptophan during the course of chemoradiation in patients with cervical cancer. Sixteen patients with histologically confirmed carcinoma of the uterine cervix, aged 53 ± 15 years (range 29–76 years), were included in this study. Plasma neopterin, kynurenine, and tryptophan were determined with an enzyme-linked immunosorbent assay. Plasma citrulline was measured with high-performance liquid chromatography. Compared to baseline, citrulline concentration was markedly and statistically significantly decreased at visits 2, 3, and 4, while returning to pretreatment concentrations at visit 5. A significant increase in serum neopterin concentrations was observed at visits 4 and 5. With the exception of decreased kynurenine/tryptophan ratio at visit 3, no significant changes were observed in the concentrations of kynurenine, tryptophan, and kynurenine/tryptophan ratio throughout the course of the treatment. In conclusion, present data demonstrate that citrulline concentrations decrease early and neopterin concentrations increase late during the course of chemoradiation in patients with cervical carcinoma. Citrulline represents a biomarker of intestinal toxicity in this population.

scholarly journals PERBANDINGAN BEBERAPA METODE ANALISIS HISTAMIN UNTUK PRODUK PERIKANAN

2009 ◽  
Vol 4 (2) ◽  
pp. 48
Author(s):  
Hedi Indra Januar
Keyword(s):  
High Performance Liquid Chromatography ◽  
Capillary Electrophoresis ◽  
Liquid Chromatography ◽  
Capillary Zone Electrophoresis ◽  
Immunosorbent Assay ◽  
High Performance ◽  
Enzyme Linked Immunosorbent Assay ◽  
Capillary Electrophoresis Capillary ◽  
Zone Electrophoresis ◽  
Capillary Zone

Studi ini  bertujuan  untuk  membandingkan  beberapa  metode dalam penentuan kadar amina  biogenik histamin. Diketahui, kadar histamin merupakan salah satu parameter yang penting  sebagai  standar  kualitas  produk  perikanan. Metode yang dibandingkan meliputi metode menggunakan spektrofluorometri, High Performance Liquid Chromatography (HPLC), Enzyme Linked Immunosorbent  Assay (ELISA), dan Capillary Electrophoresis/Capillary Zone Electrophoresis (CE/CZE). Hasil perbandingan menunjukkan bahwa baik dari sisi ketidakpastian yang mungkin ditimbulkan dari metodenya serta hasil uji kelayakan laboratorium di Eropa, metode HPLC derivatisasi post-kolom merupakan metode yang optimal saat ini untuk menentukan kadar histamin secara kuantitatif. Akan tetapi, untuk pertimbangan efisiensi waktu, maka studi ini mengusulkan bahwa penggabungan metode ELISA kualitatif dan HPLC kuantitatif sangat baik  dijadikan sebagai standar metode penentuan histamin di laboratorium pengujian produk perikanan.

scholarly journals An up-converting phosphor technology-based lateral flow assay for rapid detection of major mycotoxins in feed: Comparison with enzyme-linked immunosorbent assay and high-performance liquid chromatography-tandem mass spectrometry

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0250250
Author(s):  
Fenghua Zhu ◽  
Beibei Zhang ◽  
Lianqin Zhu
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Immunosorbent Assay ◽  
High Performance ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Current methods for detection of mycotoxin in feed are time-consuming and tedious. An up-converting phosphor technology-based lateral flow (UPT-LF) assay system is a new emerging technique for analytes detection. The aim of this study was to compare the performance of UPT-LF, an enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for detecting aflatoxin B1 (AFB1), zearalenone (ZEN) and deoxynivalenol (DON) in feed. The results showed that the use of UPT-LF for AFB1, ZEN and DON detection exhibited the following: limits of detection of 3, 50 and 200 μg/kg; average recoveries of 104.39%, 102.94% and 103.65%; and precision of 13.96%, 13.71% and 12.56%; respectively. UPT-LF required 45 min to determine one mycotoxin and 1.5 h to determine three mycotoxins in a sample, which took the shortest time. Besides, there were positive correlations between the UPT-LF, ELISA and HPLC/MS/MS methods. In conclusion, UPT-LF can be used to detect and quantify AFB1, ZEN and DON in feed samples. Though the sensitivity, accuracy and precision of UPT-LF are inferior to those of HPLC-MS/MS and ELISA, the UPT-LF assay is the most convenient and rapid technique for on-site detection among the three methods.

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