Engineering a double-rotor-based fluorescent molecule to sensitively track mitochondrial viscosity in living cells and zebrafish with high signal-to-background ratio (S/B)

2020 ◽  
Vol 401 ◽  
pp. 112789
Author(s):  
Yunzhen Yang ◽  
Longwei He ◽  
Weiying Lin
Keyword(s):  
Living Cells ◽  
High Signal ◽  
Fluorescent Molecule ◽  
Background Ratio

A novel two-photon fluorescent probe with a long Stokes shift and a high signal-to-background ratio for human NAD(P)H:quinone oxidoreductase 1 (hNQO1) detection and imaging in living cells and tissues

The Analyst ◽  
2017 ◽  
Vol 142 (14) ◽  
pp. 2624-2630 ◽  
Author(s):  
Dan Pan ◽  
Fengyan Luo ◽  
Xianjun Liu ◽  
Wei Liu ◽  
Wen Chen ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Stokes Shift ◽  
Living Cells ◽  
High Signal ◽  
Background Ratio ◽  
Two Photon

We have developed a novel TPE fluorescent probe (Q3CA-P), with a long Stokes shift and a high signal-to-background ratio, for hNQO1 detection and imaging.

Molecular Beacons for Sensitive Gene Detection in Living Cells

2003 ◽  
Author(s):  
Andrew Tsurkas ◽  
Gang Bao
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Molecular Beacon ◽  
Point Mutations ◽  
Living Cells ◽  
Molecular Beacons ◽  
High Signal ◽  
Gene Detection ◽  
Real Time Imaging ◽  
Background Ratio

Real-time imaging of gene expression in living cells has the potential to significantly impact clinical and laboratory studies of cancer, including cancer diagnosis and analysis. Molecular beacons (MBs) provide a simple and promising tool for the detection of target mRNA as tumor markers due to their high signal-to-background ratio, and their improved specificity in detecting point mutations. However, the harsh intracellular environment does limit the sensitivity of MB-based gene detection. Specifically, MBs bound to target mRNAs cannot be distinguished from those degraded by nucleases, or opened due to non-specific interactions. To overcome this difficulty, we have developed a novel dual FRET molecular beacons approach in which a pair of molecular beacons, one with a donor fluorophore and a second with an acceptor fluorophore, hybridize to adjacent regions on the same target resulting in fluorescence resonance energy transfer (FRET). The detection of a FRET signal leads to a substantially increased signal-to-background ratio compared with that in single molecular beacon assays and enables discrimination between fluorescence due to specific probe/target hybridization and a variety of false-positive events. We have performed systematic in-solution and cellular studies of dual FRET molecular beacon and demonstrated that this new approach allows for real-time imaging of gene expression in living cells.

Logic Sensing of MicroRNA in Living Cells Using DNA-Programmed Nanoparticle Network with High Signal Gain

ACS Sensors ◽  
2018 ◽  
Vol 4 (1) ◽  
pp. 250-256 ◽  
Author(s):  
Renye Yue ◽  
Zhi Li ◽  
Ganglin Wang ◽  
Junying Li ◽  
Nan Ma
Keyword(s):  
Living Cells ◽  
High Signal ◽  
Signal Gain

Efficient red luminogen with aggregation-induced emission for in vivo three-photon brain vascular imaging

2020 ◽  
Vol 4 (6) ◽  
pp. 1634-1642 ◽  
Author(s):  
Haiyan Tian ◽  
Dongyu Li ◽  
Xi Tang ◽  
Yubo Zhang ◽  
Zhiyong Yang ◽  
...  
Keyword(s):  
High Resolution ◽  
Penetration Depth ◽  
Vascular Imaging ◽  
Quantum Yields ◽  
High Signal ◽  
Aggregation Induced Emission ◽  
Background Ratio ◽  
High Quantum

DCPE-TPA exhibits three morphologies with high quantum yields and nanoparticles of DCPE-TPA are utilized for in vivo 3PF imaging, achieving a penetration depth of 300 μm with a high resolution of 1.8 μm and a high signal-to-background ratio of 14.

A spinning disk confocal microscope system for rapid high resolution, multimode, fluorescence speckle microscopy and GFP imaging in living cells

2001 ◽  
Vol 7 (S2) ◽  
pp. 8-9
Author(s):  
Paul Maddox ◽  
Julie Canman ◽  
Sonia Grego ◽  
Wendy Salmon ◽  
Clare Waterman-Storer ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescent Protein ◽  
Imaging System ◽  
Ccd Camera ◽  
High Sensitivity ◽  
Time Lapse ◽  
Living Cells ◽  
High Signal ◽  
High Quantum Efficiency ◽  
Spinning Disk

High resolution fluorescent speckle microscopy (FSM) and green fluorescent protein (GFP) imaging in living cells can require image recording at low densities of fluorophores (10 or less/resolvable unit) with low light excitation to prevent photobleaching. This needs efficient optical components, a high quantum efficiency detector, and a digital image acquisition and display system for time-lapse recording of multiple channels. Recently, Shinya and Ted Inoue have described the advantages of the Yokogawa CSU-10 spinning-disk confocal scanning unit for obtaining high quality fluorescent images with brief exposures and low fluorescence bleaching. Based on their findings, we have combined the CSU-10 unit with a high sensitivity pan-chromatic CCD camera to facilitate high spatial and temporal resolution imaging of fluorescence in living cells. in addition, the high signal-to-noise in images obtained with this instrument provides the opportunity to obtain 3-D views of extraordinary resolution and image quality after iterative constrained de-convolution.Our imaging system is constructed around a Nikon TE300 inverted microscope equipped with either a 60X or 100X Plan Apochromat objective, and standard epi-fluorescence optics for visual inspection of the specimen to locate cells for recording.

scholarly journals Fast 3D imaging of giant unilamellar vesicles using reflected light-sheet microscopy with single molecule sensitivity

2020 ◽  
Author(s):  
Sven A. Szilagyi ◽  
Moritz Burmeister ◽  
Q. Tyrell Davis ◽  
Gero L. Hermsdorf ◽  
Suman De ◽  
...  
Keyword(s):  
Single Molecule ◽  
Signal To Noise Ratio ◽  
Numerical Aperture ◽  
Light Sheet ◽  
Living Cells ◽  
High Signal ◽  
Active Optics ◽  
Single Molecule Level ◽  
Light Sheet Microscopy ◽  
Reflected Light

AbstractObservation of highly dynamic processes inside living cells at the single molecule level is key for a quantitative understanding of biological systems. However, imaging of single molecules in living cells usually is limited by the spatial and temporal resolution, photobleaching and the signal-to-background ratio. To overcome these limitations, light-sheet microscopes with thin selective plane illumination have recently been developed. For example, a reflected light-sheet design combines the illumination by a thin light-sheet with a high numerical aperture objective for single-molecule detection. Here, we developed a reflected light-sheet microscope with active optics for fast, high contrast, two-color acquisition of z-stacks. We demonstrate fast volume scanning by imaging a two-color giant unilamellar vesicle (GUV) hemisphere. In addition, the high signal-to-noise ratio enabled the imaging and tracking of single lipids in the cap of a GUV. In the long term, the enhanced reflected scanning light sheet microscope enables fast 3D scanning of artificial membrane systems and cells with single-molecule sensitivity and thereby will provide quantitative and molecular insight into the operation of cells.

scholarly journals Super-long Single Fluorescent-molecule Imaging and Tracking in Living Cells

2020 ◽  
Vol 60 (2) ◽  
pp. 113-116
Author(s):  
Taka A. TSUNOYAMA ◽  
Akihiro KUSUMI
Keyword(s):  
Living Cells ◽  
Fluorescent Molecule
Sign in / Sign up

Export Citation Format

Share Document