Spatial distribution and expression of intracellular and extracellular acid phosphatases of cluster roots at different developmental stages in white lupin

White lupin produces cluster roots in response to phosphorus deficiency. Along the cluster root, numerous short rootlets successively appear, creating a spatial and temporal gradient of developmental stages that constitutes a powerful biological model to study the dynamics of the structural and functional evolution of these organs. The present study proposes a fine histochemical, transcriptomic and functional analysis of the rootlet development from its emergence to its final length. Between these two stages, the tissue structures of the rootlets were observed, the course of transcript expressions for the genes differentially expressed was monitored and some physiological events linked to Pi nutrition were followed. A switch between (i) a growing phase, in which a normal apical meristem is present and (ii) a specialized phase for nutrition, in which the rootlet is completely differentiated, was highlighted. In the final stage of its determinate growth, the rootlet is an organ with a very active metabolism, especially for the solubilization and absorption of several nutrients. This work discusses how the transition between a growing to a determinate state in response to nutritional stresses is found in other species and underlines the fundamental dilemma of roots between soil exploration and soil exploitation.

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Localization of acid phosphatases in root cap of rice plant

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100085423 ◽

1991 ◽

Vol 49 ◽

pp. 224-225

Author(s):

Y. R. Chen ◽

Y. F. Huang ◽

W. S. Chen

Keyword(s):

Rice Plant ◽

Developmental Stages ◽

Uranyl Acetate ◽

Root Cap ◽

Plant Tissues ◽

Acid Phosphatases ◽

Root Tips ◽

Buffer Solution ◽

Cacodylate Buffer ◽

Ethanol Series

Acid phosphatases are widely distributed in different tisssues of various plants. Studies on subcellular localization of acid phosphatases show they might be present in cell wall, plasma lemma, mitochondria, plastid, vacuole and nucleus. However, their localization in rice cell varies with developmental stages of cells and plant tissues. In present study, acid phosphatases occurring in root cap are examined.Sliced root tips of ten-day-old rice(Oryza sativa) seedlings were fixed in 0.1M cacodylate buffer containing 2.5% glutaraldehyde for 2h, washed overnight in same buffer solution, incubated in Gomori's solution at 37° C for 90min, post-fixed in OsO4, dehydrated in ethanol series and finally embeded in Spurr's resin. Sections were doubly stained with uranyl acetate and lead citrate, and observed under Hitachi H-600 at 75 KV.

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STAND STRUCTURE AND SPATIAL DISTRIBUTION OF TREES AT DIFFERENT DEVELOPMENTAL STAGES AND STAND LAYERS IN MIXED STANDS IN ARTVIN REGION, TURKEY

Applied Ecology and Environmental Research ◽

10.15666/aeer/1805_61636179 ◽

2020 ◽

Vol 18 (5) ◽

pp. 6163-6179

Author(s):

A. GOKTURK ◽

H. TIRAS

Keyword(s):

Spatial Distribution ◽

Developmental Stages ◽

Stand Structure ◽

Mixed Stands

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Nodulated White Lupin Plants Growing in Contaminated Soils Accumulate Unusually High Mercury Concentrations in Their Nodules, Roots and Especially Cluster Roots

Horticulturae ◽

10.3390/horticulturae7090302 ◽

2021 ◽

Vol 7 (9) ◽

pp. 302

Author(s):

Miguel A. Quiñones ◽

Susana Fajardo ◽

Mercedes Fernández-Pascual ◽

M. Mercedes Lucas ◽

José J. Pueyo

Keyword(s):

Contaminated Soils ◽

Lupinus Albus ◽

White Lupin ◽

Cluster Roots ◽

Cluster Root ◽

Bioaccumulation Factors ◽

Aerial Parts ◽

High Mercury ◽

Lupinus Albus L ◽

Hg Accumulation

Two white lupin (Lupinus albus L.) cultivars were tested for their capacity to accumulate mercury when grown in Hg-contaminated soils. Plants inoculated with a Bradyrhizobium canariense Hg-tolerant strain or non-inoculated were grown in two highly Hg-contaminated soils. All plants were nodulated and presented a large number of cluster roots. They accumulated up to 600 μg Hg g−1 DW in nodules, 1400 μg Hg g−1 DW in roots and 2550 μg Hg g−1 DW in cluster roots. Soil, and not cultivar or inoculation, was accountable for statistically significant differences. No Hg translocation to leaves or seeds took place. Inoculated L. albus cv. G1 plants were grown hydroponically under cluster root-promoting conditions in the presence of Hg. They accumulated about 500 μg Hg g−1 DW in nodules and roots and up to 1300 μg Hg g−1 DW in cluster roots. No translocation to the aerial parts occurred. Bioaccumulation factors were also extremely high, especially in soils and particularly in cluster roots. To our knowledge, Hg accumulation in cluster roots has not been reported to date. Our results suggest that inoculated white lupin might represent a powerful phytoremediation tool through rhizosequestration of Hg in contaminated soils. Potential uptake and immobilization mechanisms are discussed.

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Update on White Lupin Cluster Root Acclimation to Phosphorus Deficiency Update on Lupin Cluster Roots

PLANT PHYSIOLOGY ◽

10.1104/pp.111.175174 ◽

2011 ◽

Vol 156 (3) ◽

pp. 1025-1032 ◽

Cited By ~ 46

Author(s):

Lingyun Cheng ◽

Bruna Bucciarelli ◽

Jianbo Shen ◽

Deborah Allan ◽

Carroll P. Vance

Keyword(s):

Phosphorus Deficiency ◽

White Lupin ◽

Cluster Roots ◽

Cluster Root

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Expression of plasma membrane H+ -ATPase in cluster roots of white lupin under phosphorus deficiency

Journal of Plant Nutrition and Soil Science ◽

10.1002/jpln.201900124 ◽

2019 ◽

Vol 182 (6) ◽

pp. 867-870

Author(s):

Sophie Stein ◽

Franziska Faust ◽

Stephan Jung ◽

Sven Schubert

Keyword(s):

Plasma Membrane ◽

Phosphorus Deficiency ◽

White Lupin ◽

Cluster Roots

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Nitric oxide is the shared signalling molecule in phosphorus- and iron-deficiency-induced formation of cluster roots in white lupin (Lupinus albus)

Annals of Botany ◽

10.1093/aob/mcs024 ◽

2012 ◽

Vol 109 (6) ◽

pp. 1055-1064 ◽

Cited By ~ 41

Author(s):

Zhi Bin Meng ◽

Li Qian Chen ◽

Dong Suo ◽

Gui Xin Li ◽

Cai Xian Tang ◽

...

Keyword(s):

Nitric Oxide ◽

Iron Deficiency ◽

Lupinus Albus ◽

White Lupin ◽

Cluster Roots ◽

Signalling Molecule

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Burkholderia Species Are Major Inhabitants of White Lupin Cluster Roots

Applied and Environmental Microbiology ◽

10.1128/aem.05845-11 ◽

2011 ◽

Vol 77 (21) ◽

pp. 7715-7720 ◽

Cited By ~ 36

Author(s):

Laure Weisskopf ◽

Stefanie Heller ◽

Leo Eberl

Keyword(s):

Burkholderia Cepacia ◽

Carbon Sources ◽

White Lupin ◽

Cluster Roots ◽

Bacterial Populations ◽

Content Type ◽

Significant Enrichment ◽

Branched Structure ◽

Successful Colonization

ABSTRACTThe formation of cluster roots by plants represents a highly efficient strategy for acquisition of sparingly available phosphate. This particular root type is characterized by a densely branched structure and high exudation of organic acids and protons, which are likely to influence the resident bacterial community. Until now, the identity of the bacterial populations living in cluster roots has not been investigated. We applied cultivation-dependent and cultivation-independent methods to characterize the dominant bacterial genera inhabiting the growing cluster roots of white lupin. We observed a high relative abundance ofBurkholderiaspecies (up to 58% of all isolated strains and 44% of all retrieved 16S rRNA sequences) and a significant enrichment with increasing cluster root age. Most of the sequences retrieved clustered together with known plant- or fungus-associatedBurkholderiaspecies, while only one of 98 sequences was affiliated with theBurkholderia cepaciacomplex.In vitroassays revealed thatBurkholderiastrains were much more tolerant to low pH than non-Burkholderiastrains. Moreover, many strains produced large amounts of siderophores and were able to utilize citrate and oxalate as carbon sources. These features seem to represent important traits for the successful colonization and maintenance ofBurkholderiaspecies in white lupin cluster roots.

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