Identification of Lysinibacillus sphaericus Binary toxin binding proteins in a malarial mosquito cell line by proteomics: A novel approach towards improving mosquito control

2020 ◽  
Vol 227 ◽  
pp. 103918
Author(s):  
Muhammad Asam Riaz ◽  
Michael J. Adang ◽  
Gang Hua ◽  
Tatiana Maria Teodoro Rezende ◽  
Antonio Mauro Rezende ◽  
...  
Keyword(s):  
Cell Line ◽  
Binding Proteins ◽  
Mosquito Control ◽  
Mosquito Cell ◽  
Binary Toxin ◽  
Lysinibacillus Sphaericus ◽  
Mosquito Cell Line ◽  
Toxin Binding ◽  
Novel Approach

scholarly journals Bacterial Toxins Active against Mosquitoes: Mode of Action and Resistance

Toxins ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 523
Author(s):  
Maria Helena Neves Lobo Silva-Filha ◽  
Tatiany Patricia Romão ◽  
Tatiana Maria Teodoro Rezende ◽  
Karine da Silva Carvalho ◽  
Heverly Suzany Gouveia de Menezes ◽  
...  
Keyword(s):  
Mode Of Action ◽  
Mosquito Control ◽  
Binary Toxin ◽  
Lysinibacillus Sphaericus ◽  
Control Programs ◽  
Resistance Selection ◽  
Single Receptor ◽  
Combined Use ◽  
Environmentally Safe ◽  
Medical Importance

Larvicides based on the bacteria Bacillus thuringiensis svar. israelensis (Bti) and Lysinibacillus sphaericus are effective and environmentally safe compounds for the control of dipteran insects of medical importance. They produce crystals that display specific and potent insecticidal activity against larvae. Bti crystals are composed of multiple protoxins: three from the three-domain Cry type family, which bind to different cell receptors in the midgut, and one cytolytic (Cyt1Aa) protoxin that can insert itself into the cell membrane and act as surrogate receptor of the Cry toxins. Together, those toxins display a complex mode of action that shows a low risk of resistance selection. L. sphaericus crystals contain one major binary toxin that display an outstanding persistence in field conditions, which is superior to Bti. However, the action of the Bin toxin based on its interaction with a single receptor is vulnerable for resistance selection in insects. In this review we present the most recent data on the mode of action and synergism of these toxins, resistance issues, and examples of their use worldwide. Data reported in recent years improved our understanding of the mechanism of action of these toxins, showed that their combined use can enhance their activity and counteract resistance, and reinforced their relevance for mosquito control programs in the future years.

Cytopathic effect of dengue-2 virus on an established mosquito cell-line

1974 ◽  
Vol 68 (1) ◽  
pp. 12
Author(s):  
M.G.R. Varma ◽  
Mary Pudney ◽  
C.J. Leake ◽  
Christine Wallace
Keyword(s):  
Cell Line ◽  
Cytopathic Effect ◽  
Mosquito Cell ◽  
Mosquito Cell Line

scholarly journals Wolbachia Infection Modifies the Profile, Shuttling and Structure of MicroRNAs in a Mosquito Cell Line

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e96107 ◽  
Author(s):  
Jaime G. Mayoral ◽  
Kayvan Etebari ◽  
Mazhar Hussain ◽  
Alexander A. Khromykh ◽  
Sassan Asgari
Keyword(s):  
Cell Line ◽  
Wolbachia Infection ◽  
Mosquito Cell ◽  
Mosquito Cell Line

Evidence that the 45-kD Glycoprotein, Part of a Putative Dengue Virus Receptor Complex in the Mosquito Cell Line C6/36, Is a Heat-Shock–Related Protein

2007 ◽  
Vol 77 (2) ◽  
pp. 283-290 ◽  
Author(s):  
Juan Salas-Benito ◽  
Jorge Reyes-Del Valle ◽  
Ivonne Ceballos-Olvera ◽  
Rosa M. del Angel ◽  
Mariana Salas-Benito ◽  
...  
Keyword(s):  
Heat Shock ◽  
Dengue Virus ◽  
Cell Line ◽  
Mosquito Cell ◽  
Receptor Complex ◽  
Related Protein ◽  
Virus Receptor ◽  
Mosquito Cell Line

A continuous mosquito cell line from larvae of Anopheles gambiae

1972 ◽  
Vol 66 (1) ◽  
pp. 21-22 ◽  
Author(s):  
Mary Pudney ◽  
Z. Marhoul ◽  
M.G.R. Varma ◽  
C.J. Leake
Keyword(s):  
Anopheles Gambiae ◽  
Cell Line ◽  
Mosquito Cell ◽  
Mosquito Cell Line

scholarly journals Isolation of Rickettsia felis in the Mosquito Cell Line C6/36

2006 ◽  
Vol 72 (2) ◽  
pp. 1705-1707 ◽  
Author(s):  
Maurício C. Horta ◽  
Marcelo B. Labruna ◽  
Edison L. Durigon ◽  
Teresinha T. S. Schumaker
Keyword(s):  
Cell Line ◽  
Protein Gene ◽  
Mosquito Cell ◽  
Rickettsia Felis ◽  
Mosquito Cell Line ◽  
Infected Cells

ABSTRACT We report the isolation and establishment of Rickettsia felis in the C6/36 cell line. Rickettsial growth was intense, always with 90 to 100% of cells being infected after few weeks. The rickettsial isolate was confirmed by testing infected cells by PCR and sequencing fragments of three major Rickettsia genes (gltA, ompB, and the 17-kDa protein gene).

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