In vitro replication of Macrobrachium rosenbergii nodavirus and extra small virus in C6/36 mosquito cell line

Abstract We established more than 200 primary cell lines of Cydia pomonella (codling moth). 81 of them were selected and screened for replication of two baculoviruses (from two different subgroups): the Choristoneura murinana NPV and the Cydia pomonella GV. Although all these cell lines had been derived from the same insect species, they varied largely in their response to challenge with the NPV. Most of them showed CPE or produced different amounts of poly-hedra. Interestingly, we also found a few cell lines that were permissive for GV replication. To our knowledge this is the first time that GV replication in cell lines has been obtained. Our results show that cell line properties are most important for baculovirus in vitro replication.

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Studies on the effect of the moulting hormone in a mosquito cell line

Journal of Insect Physiology ◽

10.1016/0022-1910(78)90124-5 ◽

1978 ◽

Vol 24 (8-9) ◽

pp. 613-621 ◽

Cited By ~ 14

Author(s):

Naomi Lanir ◽

Ephraim Cohen

Keyword(s):

Cell Line ◽

Mosquito Cell ◽

Mosquito Cell Line ◽

Moulting Hormone

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Cytopathic effect of dengue-2 virus on an established mosquito cell-line

Transactions of the Royal Society of Tropical Medicine and Hygiene ◽

10.1016/0035-9203(74)90236-3 ◽

1974 ◽

Vol 68 (1) ◽

pp. 12

Author(s):

M.G.R. Varma ◽

Mary Pudney ◽

C.J. Leake ◽

Christine Wallace

Keyword(s):

Cell Line ◽

Cytopathic Effect ◽

Mosquito Cell ◽

Mosquito Cell Line

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Wolbachia Infection Modifies the Profile, Shuttling and Structure of MicroRNAs in a Mosquito Cell Line

PLoS ONE ◽

10.1371/journal.pone.0096107 ◽

2014 ◽

Vol 9 (4) ◽

pp. e96107 ◽

Cited By ~ 43

Author(s):

Jaime G. Mayoral ◽

Kayvan Etebari ◽

Mazhar Hussain ◽

Alexander A. Khromykh ◽

Sassan Asgari

Keyword(s):

Cell Line ◽

Wolbachia Infection ◽

Mosquito Cell ◽

Mosquito Cell Line

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Evidence that the 45-kD Glycoprotein, Part of a Putative Dengue Virus Receptor Complex in the Mosquito Cell Line C6/36, Is a Heat-Shock–Related Protein

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.2007.77.283 ◽

2007 ◽

Vol 77 (2) ◽

pp. 283-290 ◽

Cited By ~ 47

Author(s):

Juan Salas-Benito ◽

Jorge Reyes-Del Valle ◽

Ivonne Ceballos-Olvera ◽

Rosa M. del Angel ◽

Mariana Salas-Benito ◽

...

Keyword(s):

Heat Shock ◽

Dengue Virus ◽

Cell Line ◽

Mosquito Cell ◽

Receptor Complex ◽

Related Protein ◽

Virus Receptor ◽

Mosquito Cell Line

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A continuous mosquito cell line from larvae of Anopheles gambiae

Transactions of the Royal Society of Tropical Medicine and Hygiene ◽

10.1016/0035-9203(72)90039-9 ◽

1972 ◽

Vol 66 (1) ◽

pp. 21-22 ◽

Cited By ~ 2

Author(s):

Mary Pudney ◽

Z. Marhoul ◽

M.G.R. Varma ◽

C.J. Leake

Keyword(s):

Anopheles Gambiae ◽

Cell Line ◽

Mosquito Cell ◽

Mosquito Cell Line

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Isolation of Rickettsia felis in the Mosquito Cell Line C6/36

Applied and Environmental Microbiology ◽

10.1128/aem.72.2.1705-1707.2006 ◽

2006 ◽

Vol 72 (2) ◽

pp. 1705-1707 ◽

Cited By ~ 33

Author(s):

Maurício C. Horta ◽

Marcelo B. Labruna ◽

Edison L. Durigon ◽

Teresinha T. S. Schumaker

Keyword(s):

Cell Line ◽

Protein Gene ◽

Mosquito Cell ◽

Rickettsia Felis ◽

Mosquito Cell Line ◽

Infected Cells

ABSTRACT We report the isolation and establishment of Rickettsia felis in the C6/36 cell line. Rickettsial growth was intense, always with 90 to 100% of cells being infected after few weeks. The rickettsial isolate was confirmed by testing infected cells by PCR and sequencing fragments of three major Rickettsia genes (gltA, ompB, and the 17-kDa protein gene).

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