Structure of the DNA-binding domain of human myelin-gene regulatory factor reveals its potential protein-DNA recognition mode

2018 ◽  
Vol 203 (2) ◽  
pp. 170-178 ◽  
Author(s):  
Baohua Chen ◽  
Yun Zhu ◽  
Sheng Ye ◽  
Rongguang Zhang
Author(s):  
WenYu Wu ◽  
Xiangkai Zhen ◽  
Ning Shi

An extra affiliation is added for the authors of the article by Wu et al. [(2017), Acta Cryst. F73, 393–397].


2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Xiangkai Zhen ◽  
Bowen Li ◽  
Fen Hu ◽  
Shufeng Yan ◽  
Gabriele Meloni ◽  
...  

Author(s):  
WenYu Wu ◽  
Xiangkai Zhen ◽  
Ning Shi

The myelin sheath, which envelops axons in the vertebrate central nervous system, is crucial for the rapid conduction of action potentials. Myelin-gene regulatory factor (MRF) is a recently identified transcription factor that is required for myelin-sheath formation. Loss of MRF leads to demyelinating diseases and motor learning deficiency. MRF is a membrane-bound transcription factor that undergoes autocleavage from the endoplasmic reticulum membrane. The N-terminus of MRF contains a DNA-binding domain (DBD) that functions as a homotrimer. In this study, the MRF DBD was cloned, purified and crystallized in order to understand the molecular mechanism that regulates the transcription of myelin genes. Selenomethionine was subsequently introduced into the crystals to obtain the phases for the MRF DBD structure. The native and selenomethionine-labelled crystals exhibited diffraction to 2.50 and 2.51 Å resolution, respectively. The crystals belonged to space groupP321 and the selenomethionine-labelled crystals had unit-cell parametersa= 104.0,b= 104.0,c= 46.7 Å, α = 90, β = 90, γ = 120°. The calculated Matthews coefficient was 3.04 Å3Da−1and the solvent content was 59.5%, indicating the presence of one MRF DBD molecule in the asymmetric unit.


FEBS Letters ◽  
1995 ◽  
Vol 359 (2-3) ◽  
pp. 184-188 ◽  
Author(s):  
Koichi Uegaki ◽  
Masahiro Shirakawa ◽  
Hisashi Harada ◽  
Tadatsugu Taniguchi ◽  
Yoshimasa Kyogoku

Oncogene ◽  
2000 ◽  
Vol 19 (11) ◽  
pp. 1411-1418 ◽  
Author(s):  
Y R Rubinstein ◽  
P H Driggers ◽  
V V Ogryzko ◽  
A M Thornton ◽  
K Ozato ◽  
...  

1994 ◽  
Vol 70 (10) ◽  
pp. 200-204
Author(s):  
Koichi UEGAKI ◽  
Masahiro SHIRAKAWA ◽  
Hisashi HARADA ◽  
Tadatsugu TANIGUCHI ◽  
Yoshimasa KYOGOKU

1996 ◽  
Vol 3 (2) ◽  
pp. 178-187 ◽  
Author(s):  
Kazuhiro Ogata ◽  
Chie Kanei-Ishii ◽  
Motoko Sasaki ◽  
Hideki Hatanaka ◽  
Aritaka Nagadoi ◽  
...  

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