scholarly journals Anticancer efficacies of Krameria lappacea extracts against human breast cancer cell line (MCF-7): Role of oxidative stress and ROS generation

2021 ◽  
Vol 29 (3) ◽  
pp. 244-251
Author(s):  
Mai Mohammad Al-Oqail
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Breast Cancer Cell Line ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Human Breast Cancer Cell ◽  
Ros Generation ◽  
Human Breast ◽  
Mcf 7

c-myb mediates inflammatory reaction against oxidative stress in human breast cancer cell line, MCF-7

2011 ◽  
Vol 29 (8) ◽  
pp. 686-693 ◽  
Author(s):  
Govinda Bhattarai ◽  
Young-Hee Lee ◽  
Nan-Hee Lee ◽  
Ji-Soo Yun ◽  
Pyoung-Han Hwang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Cell Line ◽  
Inflammatory Reaction ◽  
Breast Cancer Cell Line ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
Mcf 7

scholarly journals The effect of Topotecan on oxidative stress in MCF-7 human breast cancer cell line.

2005 ◽  
Vol 52 (4) ◽  
pp. 897-902 ◽  
Author(s):  
Mujgan Timur ◽  
S Halide Akbas ◽  
Tomris Ozben
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Breast Cancer Cell Line ◽  
Protein Oxidation ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
Mcf 7

Topotecan, a semisynthetic water-soluble derivative of camptothecin exerts its cytotoxic effect by inhibiting topoisomerase I and causes double-strand DNA breaks which inhibit DNA function and ultimately lead to cell death. In previous studies it was shown that camptothecin causes ROS formation. The aim of this study was to investigate if Topotecan like camptotecin causes oxidative stress in MCF-7 human breast cancer cell line. Determining the oxidant effect of Topotecan may elucidate a possible alternative mechanism for its cytotoxicity. MCF-7 cells were cultured and exposed to Topotecan for 24 h at 37 degrees C. The viability of the cells (% of control) was measured using the colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Lipid peroxidation (TBARS), protein oxidation (carbonyl content), sulfhydryl, glutathione (GSH) levels, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were determined in MCF-7 cells with and without Topotecan incubation. We found the IC(50) concentration of Topotecan as 0.218 microM in MCF-7 cells. This concentration of Topotecan was used in the incubations of the cells. Our data indicated increased oxidative status, as revealed by increased lipid peroxidation and protein oxidation, and decreased GSH and sulfhydryl levels in MCF-7 cells exposed to Topotecan compared to control cells. In contrast, there was a slight increase in SOD and a significant increase in GPx and catalase activity in MCF-7 cells incubated with Topotecan compared to the control. These results support our hypothesis that Topotecan increases oxidative stress in MCF-7 cells.

Design, Synthesis and In Vitro Evaluation of 18β-Glycyrrhetinic Acid Derivatives for Anticancer Activity Against Human Breast Cancer Cell Line MCF-7

2014 ◽  
Vol 21 (9) ◽  
pp. 1160-1170 ◽  
Author(s):  
Dharmendra Yadav ◽  
Komal Kalani ◽  
Abhishek Singh ◽  
Feroz Khan ◽  
Santosh Srivastava ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Anticancer Activity ◽  
Breast Cancer Cell Line ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
Design Synthesis ◽  
Mcf 7

Stable expression of cytochrome P450IIIA7 cDNA in human breast cancer cell line MCF-7 and its application to cytotoxicity testing

1992 ◽  
Vol 292 (1) ◽  
pp. 136-140 ◽  
Author(s):  
Ryuji Kitamura ◽  
Keiko Sato ◽  
Minoru Sawada ◽  
Susumu Itoh ◽  
Mitsukazu Kitada ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
Mcf 7
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