066 Revactin®, a Nutraceutical Combination, Activates the iNOS/cGMP Pathway in Human Corporal Smooth Muscle Cells

2021 ◽  
Vol 18 (3) ◽  
pp. S36
Author(s):  
L. Graciano ◽  
A. Abraham ◽  
S. Nguyen ◽  
J.N. Mills ◽  
M. Ferrini
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Cgmp Pathway

076 Exogenous Administration of L-arginine Does Not Stimulate the Endogenous NO-cGMP Pathway in Rat Penile Corpora Smooth Muscle Cells

2019 ◽  
Vol 16 (4) ◽  
pp. S40
Author(s):  
M. Ferrini ◽  
A. Abraham ◽  
S. Nguyen ◽  
M. Flores ◽  
R. Luna ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Cgmp Pathway

Regulation of the nitric oxide synthase-nitric oxide- cGMP pathway in rat mesenteric endothelial cells

2001 ◽  
Vol 91 (6) ◽  
pp. 2553-2560 ◽  
Author(s):  
Andreas Papapetropoulos ◽  
Stavroula Andreopoulos ◽  
Carolyn Y. Go ◽  
Azizul Hoque ◽  
Leslie C. Fuchs ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Inos Mrna ◽  
Microtubule Depolymerization ◽  
Inos Protein Expression ◽  
Cgmp Pathway ◽  
Inflammatory Stimuli

Most of the available data on the nitric oxide (NO) pathway in the vasculature is derived from studies performed with cells isolated from conduit arteries. We investigated the expression and regulation of components of the NO synthase (NOS)-NO-cGMP pathway in endothelial cells from the mesenteric vascular bed. Basally, or in response to bradykinin, cultured mesenteric endothelial cells (MEC) do not release NO and do not express endothelial NOS protein. MEC treated with cytokines, but not untreated cells, express inducible NOS (iNOS) mRNA and protein, increase nitrite release, and stimulate cGMP accumulation in reporter smooth muscle cells. Pretreatment of MEC with genistein abolished the cytokine-induced iNOS expression. On the other hand, exposure of MEC to the microtubule depolymerizing agent colchicine did not affect the cytokine-induced increase in nitrite formation and iNOS protein expression, whereas it inhibited the induction of iNOS in smooth muscle cells. Collectively, our findings demonstrate that MEC do not express endothelial NOS but respond to inflammatory stimuli by expressing iNOS, a process that is blocked by tyrosine kinase inhibition but not by microtubule depolymerization.

scholarly journals Activation of the iNOS/NO/cGMP pathway by Revactin® in human corporal smooth muscle cells

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Monica G. Ferrini ◽  
Andrea Abraham ◽  
Leslie Graciano ◽  
Sabine Nguyen ◽  
Jesse N. Mills ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Cgmp Pathway

Immunogold localization of HMB-45 antigen in pulmonary lymphangiomyomatosis

1995 ◽  
Vol 53 ◽  
pp. 998-999
Author(s):  
J.M. Minda ◽  
E. Dessy ◽  
G. G. Pietra
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Muscle Cells ◽  
Ultrastructural Localization ◽  
Reproductive Age ◽  
Thin Sections ◽  
Smooth Muscle Proliferation ◽  
Hmb 45

Pulmonary lymphangiomyomatosis (PLAM) is a rare disease occurring exclusively in women of reproductive age. It involves the lungs, lymph nodes and lymphatic ducts. In the lungs, it is characterized by the proliferation of smooth muscle cells around lymphatics in the bronchovascular bundles, lobular septa and pleura The nature of smooth muscle proliferation in PLAM is still unclear. Recently, reactivity of the smooth muscle cells for HMB-45, a melanoma-related antigen has been reported by immunohistochemistry. The purpose of this study was the ultrastructural localization of HMB-45 immunoreactivity in these cells using gold-labeled antibodies.Lung tissue from three cases of PLAM, referred to our Institution for lung transplantation, was embedded in either Poly/Bed 812 post-fixed in 1% osmium tetroxide, or in LR White, without osmication. For the immunogold technique, thin sections were placed on Nickel grids and incubated with affinity purified, monoclonal anti-melanoma antibody HMB-45 (1:1) (Enzo Diag. Co) overnight at 4°C. After extensive washing with PBS, grids were treated with Goat-anti-mouse-IgG-Gold (5nm) (1:10) (Amersham Life Sci) for 1 hour, at room temperature.

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