Evaluation of the anti-inflammatory activity of the aqueous and ethanolic extracts of the leaves of Albizzia lebbeck in rats

Inflammation is a body reaction which embroils cellular and biochemical responses, which is not only symptom for shared diseases but also known to be an initial phase for certain serious Alzheimer’s, cancer, heart vascular diseases. In order to overcome these drawbacks, there is an urgent need for nutraceuticals with excellent anti-inflammatory response with minimum side effects. Aim: An attempt has been made to evaluate the anti-inflammatory activity along with gene expression analysis on ethanolic extracts of Crocus sativus (CSEE). Dried stigmas of C. sativus were analyzed for anti-inflammatory activity by macrophage scavenging assay. In this study, the phagocytic activity of the extract was tested on oxidative burst reduction of macrophages. RT-PCR was performed to analyze the anti-apoptotic gene expression during cell death, as a result of the compound treatment on cancer cells. The CSEE unveiled high phagocytic activity on the oxidative burst reduction, presenting intracellular killing and the enhancement of lysosomal enzyme activity, showing the active degranulation of macrophages. These findings suggest that C. sativus possessed excellent anti-inflammatory as well as apoptotic activities. Hence it was proposed that C. sativus could be exploited against oxidative stress, anti-inflammatory, cancer and ageing therapy to justify their use in traditional medicine as a nutraceutical.

Download Full-text

Chymase inhibition: a key factor in the anti-inflammatory activity of ethanolic extracts and spilanthol isolated from Acmella oleracea

Journal of Ethnopharmacology ◽

10.1016/j.jep.2020.113610 ◽

2020 ◽

pp. 113610

Author(s):

Renan Stein ◽

Markus Berger ◽

Bianca Santana de Cecco ◽

Luana Peixoto Mallmann ◽

Paula Barros Terraciano ◽

...

Keyword(s):

Inflammatory Activity ◽

Ethanolic Extracts ◽

Key Factor ◽

Anti Inflammatory

Download Full-text

Phytochemical and Anti-Inflammatory Evaluation of Herbal Gel Prepared from Bark Extract of Mesua Ferrea Linn

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v9i5-s.3638 ◽

2019 ◽

Vol 9 (5-s) ◽

pp. 53-56

Author(s):

Harjeet Singh ◽

B. K. Sarkar ◽

Jagdish Chandra Arya ◽

Shashi Pal ◽

Ravi Kumar ◽

...

Keyword(s):

Research Work ◽

Stem Bark ◽

Inflammatory Activity ◽

Bark Extract ◽

Phytochemical Analysis ◽

Drug Study ◽

Standard Drug ◽

Ethanolic Extracts ◽

Anti Inflammatory

The present research work involves formulation of herbal gel containing stem bark extracts of Mesua ferrea Linn and its evaluation for in vitro anti-inflammatory activity. The gel formulations were prepared using ethanolic extracts along with different polymer. The physiochemical parameters of formulations like; pH, viscosity and spreadability etc. also evaluated. Phytochemical analysis revealed presence of phenols, flavonoids and alkaloids, etc. The ethanolic extracts showed appreciable anti-inflammatory activity compared to the standard drug. Study confirmed that potential anti-inflammatory formulation can be developed from bark extract of Mesua ferrea Linn. Keywords: Mesua ferrea Linn., Anti-inflammatory, Herbal Gel, Phytochemical, Albumin denaturation.

Download Full-text

Analgesic and anti-inflammatory activity of ethanolic extracts of leaf and fruit pulp of Aegle marmelos in albino rats

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20172634 ◽

2017 ◽

Vol 6 (7) ◽

pp. 1596

Author(s):

Krishnakanth K. ◽

Abhilash B. G. ◽

Neeraja K. ◽

Hari Jagannadha Rao G. ◽

Chakrapani Cheekavolu

Keyword(s):

Methyl Cellulose ◽

Inflammatory Activity ◽

Albino Rats ◽

Latency Time ◽

Fruit Pulp ◽

Paw Edema ◽

Aegle Marmelos ◽

Hot Plate ◽

Ethanolic Extracts ◽

Anti Inflammatory

Background: To study the analgesic and anti-inflammatory activity of the ethanolic extracts of leaf (LE) and fruit pulp (FE) of Aegle marmelos in comparison with the standard drugs pentazocine and diclofenac in albino rats. 56 healthy wistar albino rats of either sex were randomly divided into 14 groups of 4 each (n=4), weighing about 150-200grams were selected for the study. All drugs i.e. 1ml of 1% Carboxy methyl cellulose (CMC), pentazocine 10mg/kg (intraperitoneal), diclofenac 10 mg/kg and LE and FE at doses of 100mg/kg and 200mg/kg in 1% CMC (vehicle) were administered per oral one hour before the experiment.Methods: Analgesic activity was assessed by Eddy’s hot plate method (latency time) and acetic acid induced writhing (number of writhings) method. Anti-inflammatory activity was assessed by carrageenan induced hind paw edema method using digital plethysmometer.Results: Test drugs at doses LE 200mg/kg and FE 200mg/kg have shown significant increase in mean latency time in Eddy’s hot plate (P <0.001) and decrease in the average number of writhing movements (P <0.01). LE 200 and FE 200 doses did not show significant anti-inflammatory activity in Carrageenan induced paw edema model (P >0.05).Conclusions: Leaf extract (LE) and fruit extract (FE) at doses 200mg/kg have shown significant analgesic action and no anti-inflammatory action.

Download Full-text

Elsholtzia ciliata (Thunb.) Hyl. Extracts from Different Plant Parts: Phenolic Composition, Antioxidant, and Anti-Inflammatory Activities

Molecules ◽

10.3390/molecules25051153 ◽

2020 ◽

Vol 25 (5) ◽

pp. 1153 ◽

Cited By ~ 5

Author(s):

Lauryna Pudziuvelyte ◽

Mindaugas Liaudanskas ◽

Aiste Jekabsone ◽

Ilona Sadauskiene ◽

Jurga Bernatoniene

Keyword(s):

Phenolic Compounds ◽

Prostaglandin E2 ◽

High Performance ◽

Inflammatory Activity ◽

Antioxidant Efficiency ◽

Plant Parts ◽

Ethanolic Extracts ◽

Elsholtzia Ciliata ◽

Health Promoting ◽

Anti Inflammatory

Polyphenols play an important role on the health-promoting properties of humans. Plants belonging to Lamiaceae family are known as rich source of phenolic compounds. The current work aimed to evaluate the phenolic compounds, antioxidant, and anti-inflammatory activity of Elsholtzia ciliata (Thunb.) Hyl. ethanolic extracts from leaf, stem, flower, and whole herb. Twelve compounds were identified in ethanolic extracts using high-performance liquid chromatography (HPLC). The HPLC analysis revealed that chlorogenic acid, rosmarinic acid, and rutin were predominant compounds in ethanolicic extracts. Using HPLC-ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) post-column assay, avicularin, chlorogenic, and rosmarinic acids were identified as the predominant radical scavengers in all ethanolic extracts. All tested preparations significantly reduced the level of secretion of proinflammatory cytokines TNF-α, IL-6, and prostaglandin E2 induced by lipopolysaccharide treatment in mouse peritoneal macrophage cell culture. Stem and flower extracts were most efficient in reducing cytokine release, but leaf extract demonstrated stronger effect on prostaglandin E2 secretion. This is the first study exploring antioxidant efficiency by HPLC–ABTS post-column method and investigating anti-inflammatory activity of ethanolic extracts from E. ciliata different plant parts.

Download Full-text