scholarly journals Nonviral gene transfer of hepatocyte growth factor attenuates neurologic injury after spinal cord ischemia in rabbits

2006 ◽  
Vol 132 (4) ◽  
pp. 941-947 ◽  
Author(s):  
Enyi Shi ◽  
Xiaojing Jiang ◽  
Teruhisa Kazui ◽  
Naoki Washiyama ◽  
Katsushi Yamashita ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Growth Factor ◽  
Gene Transfer ◽  
Hepatocyte Growth Factor ◽  
Spinal Cord Ischemia ◽  
Neurologic Injury ◽  
Nonviral Gene Transfer ◽  
Hepatocyte Growth

scholarly journals Intrathecal Injection of Hepatocyte Growth Factor Gene-modified Marrow Stromal Cells Attenuates Neurologic Injury Induced by Transient Spinal Cord Ischemia in Rabbits

2010 ◽  
Vol 113 (5) ◽  
pp. 1109-1117 ◽  
Author(s):  
Enyi Shi ◽  
Xiaojing Jiang ◽  
Lingling Wang ◽  
Satoshi Akuzawa ◽  
Yoshiki Nakajima ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Motor Neurons ◽  
Stromal Cells ◽  
Spinal Cord Ischemia ◽  
Intrathecal Injection ◽  
Marrow Stromal Cells ◽  
Infrarenal Aorta ◽  
Hepatocyte Growth

Background Our previous studies showed that transfer of hepatocyte growth factor (HGF) gene or transplantation of marrow stromal cells (MSCs) remarkably attenuated neurologic injuries after spinal cord ischemia. We sought to investigate a novel neuroprotective strategy of transplantation of human HGF gene-modified MSCs on ischemic spinal cords. Methods Human HGF gene was transferred into MSCs in vitro. The HGF gene-modified MSCs were transplanted by means of intrathecal injection. Two days later, spinal cord ischemia was induced by occlusion of the infrarenal aorta with a balloon catheter for 40 or 50 min. Hind-limb motor function was assessed during a 14-day recovery period with Tarlov criteria, and then histologic examination was performed. Results Human HGF was detected in the cerebrospinal fluid from 2 to 16 days after transplantation of HGF gene-modified MSCs. Compared with the controls, transplantation of HGF gene-modified MSCs or MSCs alone significantly improved the Tarlov scores 1, 2, 7, and 14 days after spinal cord ischemia of 40 or 50 min (P < 0.01, respectively) and increased the number of intact motor neurons in the lumbar spinal cord (P < 0.01, respectively). When the ischemic period was extended to 50 min, the Tarlov scores and the number of intact motor neurons of rabbits transplanted with HGF gene-modified MSCs were markedly higher than those of the rabbits transplanted with MSCs only (P < 0.05, respectively). Conclusions Transplantation of HGF gene-modified MSCs induces powerful neuroprotection on spinal cords against ischemia-reperfusion injury and is more therapeutically efficient than transplantation of MSCs only.

scholarly journals Efficacy of nonviral gene transfer of human hepatocyte growth factor (HGF) against ischemic-reperfusion nerve injury in rats

PLoS ONE ◽  
2020 ◽  
Vol 15 (8) ◽  
pp. e0237156
Author(s):  
Toyokazu Tsuchihara ◽  
Hitoshi Nukada ◽  
Kuniaki Nakanishi ◽  
Ryuichi Morishita ◽  
Masatoshi Amako ◽  
...  
Keyword(s):  
Growth Factor ◽  
Gene Transfer ◽  
Hepatocyte Growth Factor ◽  
Nerve Injury ◽  
Human Hepatocyte ◽  
Nonviral Gene Transfer ◽  
Ischemic Reperfusion ◽  
Human Hepatocyte Growth Factor ◽  
Hepatocyte Growth

scholarly journals 1868: Prevention of Chronic Allograft Nephropathy(CAN) With Hepatocyte Growth Factor (HGF) Gene Transfer

2004 ◽  
Vol 171 (4S) ◽  
pp. 493-493
Author(s):  
Ryoichi Imamura ◽  
Shiro Takahara ◽  
Yukiomi Namba ◽  
Yi Shi ◽  
Koji Yazawa ◽  
...  
Keyword(s):  
Growth Factor ◽  
Gene Transfer ◽  
Hepatocyte Growth Factor ◽  
Chronic Allograft Nephropathy ◽  
Hepatocyte Growth

scholarly journals Multipotent Neurotrophic Effects of Hepatocyte Growth Factor in Spinal Cord Injury

2019 ◽  
Vol 20 (23) ◽  
pp. 6078 ◽  
Author(s):  
Kentaro Yamane ◽  
Haruo Misawa ◽  
Tomoyuki Takigawa ◽  
Yoshihiro Ito ◽  
Toshifumi Ozaki ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Stem Cell Differentiation ◽  
Neural Regeneration ◽  
Therapeutic Effects ◽  
Positive Effects ◽  
Cord Injury ◽  
Hepatocyte Growth

Spinal cord injury (SCI) results in neural tissue loss and so far untreatable functional impairment. In addition, at the initial injury site, inflammation induces secondary damage, and glial scar formation occurs to limit inflammation-mediated tissue damage. Consequently, it obstructs neural regeneration. Many studies have been conducted in the field of SCI; however, no satisfactory treatment has been established to date. Hepatocyte growth factor (HGF) is one of the neurotrophic growth factors and has been listed as a candidate medicine for SCI treatment. The highlighted effects of HGF on neural regeneration are associated with its anti-inflammatory and anti-fibrotic activities. Moreover, HGF exerts positive effects on transplanted stem cell differentiation into neurons. This paper reviews the mechanisms underlying the therapeutic effects of HGF in SCI recovery, and introduces recent advances in the clinical applications of HGF therapy.

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