Objective: The aim of this study was to assess the histological reaction of the subcutaneous tissue of rats after the implantation of natural inorganic mineral scaffold from a calf femur containing recombinant human bone morphogenetic protein (rhBMP-2) and irradiated with low-power laser light. Material and methods: Sixteen Wistar rats were incised in the torso in a medial-longitudinal orientation, and the subcutaneous tissue of the left and right sides of the incision was pulled apart for implantation of the inorganic bone scaffold containing rhBMP-2. Diode laser light was applied to the right side implant at a dose of 8 J/cm2 for 3 minutes, forming two groups: G1 (control) and G2 (irradiated with laser). Implants and surrounding tissue were removed from four animals on days 7, 21, 40 and 112 for microscopic study. The histological results were assessed by means of grading (0 = absence, 1 = slight presence, 2 = representative and 3 = very representative), considering the following events: formation of osteoid structure, acute inflammation, chronic inflammation, fibrin deposition, neovascularization, foreign body granuloma and fibrosis. Results: The results showed no statistically significant differences in each of the four periods when the two groups were compared (p>0.05 – Mann-Whitney’s test). Conclusion: The natural inorganic scaffold from a calf femur with rhBMP-2 was a biocompatible combination. Under these conditions, the inductive capacity of rhBMP-2 for cell differentiation was inhibited. A slight hastening of tissue healing was shown in the group that was irradiated with low-power laser light.
P1.16-010 Development of a Novel Surgical Marking Method Using Low Power Laser Light
