Re: Sperm Vacuoles Cannot Help to Differentiate Fertile Men from Infertile Men with Normal Sperm Parameter Values

Introduction. Semen analysis is considered as the surrogate marker for male fecundity while assessing infertile men. There are several reasons for altered semen quality and bacteriospermia could be one among them. Thereby the aim of our work is to study the semen culture and its impact on semen parameters among infertile men.Materials and Methods. Semen samples were collected from men attending infertility clinic. Semen parameters were analysed based on WHO guidelines. Also, samples were subjected to culture using standard bacteriological techniques.Results. A total of 85 samples were collected. A number of 47 (55.30%) had normal sperm count, 37 (43.50%) had oligozoospermia, and one (1.17%) had azoospermia. Teratozoospermia was the most common abnormality observed (81.17%) followed by asthenozoospermia (28.23%). The prevalence of bacteriospermia was 35.3%.Enterococcus faecalis(30%) was the most common organism isolated followed by Coagulase negativeStaphylococcus(23.33%),Staphylococcus aureus(20%), andE. coli(10%). Other less frequently isolated organisms wereKlebsiella pneumoniae(6.66%),Proteussp. (6.66%), andCitrobactersp. (3.33%).Conclusion. The presence of asymptomatic bacteriospermia did not correlate with abnormal semen parameters.

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Diagnostic value of bioactive FSH in male infertility

Acta Endocrinologica ◽

10.1530/acta.0.1210802 ◽

1989 ◽

Vol 121 (6) ◽

pp. 802-810 ◽

Cited By ~ 22

Author(s):

Friedrich Jockenhövel ◽

Shafiq A. Khan ◽

Eberhard Nieschlag

Keyword(s):

Diagnostic Value ◽

Obstructive Azoospermia ◽

Severe Oligozoospermia ◽

Infertile Men ◽

Normal Sperm ◽

Sperm Counts ◽

Number Of Patients ◽

Patient Groups

Abstract. Bioactive FSH and immunoreactive FSH were determined in 193 infertile men and in 23 men with proven fertility using the Sertoli cell aromatase bioassay for bioactive FSH measurement and a two-site fluoroimmunoassay for immunoreactive FSH measurement. Overall bioactive and immunoreactive FSH levels correlated well (r = 0.74, p < 0.001) but were significantly different from fertile men (bioactive FSH: 6.2 ± 0.3 U/l; immunoreactive FSH: 4.1 ± 0.4 U/l) in patients with Klinefelter's syndrome (24.1 ± 6.1; 26.9 ± 3.0), non-obstructive azoospermia (25.1 ± 4.3; 22.2 ± 4.0), maldescended testes (12.5 ± 4.6; 14.6 ± 1.6), and patients with severe oligozoospermia (11.9 ± 1.2; 11.2 ± 1.0). Infertile men with moderate oligozoospermia (8.9 ± 1.5; 8.0 ± 1.1) and normal sperm counts (9.6 ± 1.1; 7.6 ± 1.0) had insignificantly elevated bioactive FSH and immunoreactive FSH levels. Bioactive to immunoreactive FSH ratios were significantly reduced in all patient groups except for patients with normal sperm counts when compared with fertile men. A considerable number of patients exhibited elevated immunoreactive FSH concomitant with normal bioactive FSH levels. We conclude that 1. determination of immunoreactive FSH suffices for classification of patients; 2. bioactive to immunoreactive FSH ratios are reduced in infertile men; 3. some men might secrete immunoreactive FSH with reduced bioactivity.

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Restoration of normal sperm characteristics in hypoprolactinemic infertile men treated with metoclopramide and exogenous human prolactin

Clinical Pharmacology & Therapeutics ◽

10.1016/0009-9236(95)90253-8 ◽

1995 ◽

Vol 58 (3) ◽

pp. 354-359 ◽

Cited By ~ 10

Author(s):

C. S. Ufearo ◽

O. E. Orisakwe

Keyword(s):

Infertile Men ◽

Normal Sperm ◽

Sperm Characteristics ◽

Human Prolactin

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The impact of obesity on seminal fluid in patients with male infertility

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v9ispl1.1333 ◽

2018 ◽

Vol 9 (SPL1) ◽

Author(s):

Ahmed T Alahmar ◽

Zahraa Ali ◽

Zahraa Muhsin ◽

Hadeel Qasim

Keyword(s):

Sperm Morphology ◽

Seminal Fluid ◽

Sperm Concentration ◽

Cross Sectional Study ◽

Normal Weight ◽

Cross Sectional ◽

Progressive Motility ◽

Infertile Men ◽

Normal Sperm ◽

The Impact

Data on the effect of obesity on seminal fluid and men fertility are inconsistent. The aim of this study was to evaluate the impact of body mass index (BMI) on semen characteristics in infertile men.A cross-sectional study was conducted on seventy-four infertile men who met inclusion criteria.Semen sample were collected and sperm concentration,progressive motility,total motility and normal sperm morphology were assessed in accordance with WHO 2010 criteria. For each patient weight and height were measure and patients were divided by BMI into normal weight (BMI: 18.5–24.9 kg/m2,n=30),overweight (BMI: 25–29.9 kg/m2,n=30) and obese (BMI:≥30 kg/m2, n=14). Seminal fluid parameters were compared among the three groups.Sperm concentration was lower in obese men but it did not differ significantly from those of normal weight and overweight infertile men (25.71±22.16,34.33±31.11,36.07±31.24 and million/ml respectively,P>0.05). Sperm progressive motility,total motility and normal sperm morphology also were not significantly different among normal weight,overweight and obese infertile men.Our findings suggest that BMI may have no influence on sperm concentration,motility and normal morphology in infertile men.

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195 CORRELATION BETWEEN SPERM PENETRATION ASSAY AND IN VITRO FERTILIZATION USING HAMSTER AND HUMAN OOCYTES

Reproduction Fertility and Development ◽

10.1071/rdv20n1ab195 ◽

2008 ◽

Vol 20 (1) ◽

pp. 176

Author(s):

S. Al-Anssari ◽

A. Taiyeb ◽

M. Ridha-Albarzanchi ◽

Z. Qurjia ◽

S. Al-Atraqchi ◽

...

Keyword(s):

Sperm Morphology ◽

Seminal Fluid ◽

Age Groups ◽

Human Oocytes ◽

Ivf Outcomes ◽

Fluid Analysis ◽

Infertile Men ◽

Normal Sperm ◽

Sperm Penetration ◽

Sperm Penetration Assay

The objective of this study was to evaluate the diagnostic and therapeutic significance of a sperm penetration assay (SPA) for predicting IVF outcomes in different age groups of infertile male patients. The patients were divided into 4 age groups, including a first fertile control group (G1: n = 20, aged 20 to 60 yr; G2: n = 25, aged 20 to 30 yr; G3: n = 40, aged 31 to 40 yr; and G4: n = 10, aged >41 yr). Seminal fluid analysis, SPA, and IVF were performed. Sperm concentration, motility, motility grade, and morphology were significantly (P < 0.01) higher in G1 compared with the other groups. Sperm parameters in G4 were also significantly (P < 0.05) lower compared with other groups, except in the sperm morphology percentages. Sperm penetration rate (SPR) was significantly higher in G2 v. G3 and G4 (31.6 v. 26.6 and 24.2, respectively; P < 0.05). The sperm penetration index was similar in G2, G3, and G4 (P > 0.05). The SPR in hamster oocytes was positively correlated with the sperm grade of motility (r = 0.80, P < 0.01), and normal sperm morphology (r = 0.81, P < 0.01) in infertile men (n = 75). The sperm penetration percentage in human oocytes was positively correlated with sperm motility, grade of motility, and normal sperm morphology (r = 60, P < 0.01) in infertile men. No positive correlation with human sperm concentrations was observed in hamster and human oocytes (r = 0.34, P < 0.01). The IVF rates were significantly, positively correlated with SPR of zona-free hamster oocytes (P < 0.001, r = 0.7). The results of the present study demonstrate that the SPA is a more sensitive screening tool than the seminal fluid analysis for predicting IVF outcomes, and it is positively correlated with human IVF scores. The authors thank Baghdad University and the director of Baghdad Teaching Hospital for their kind cooperation. The authors also thank the Ministry of Health and the Ministry of Higher Education and Scientific Research for their financial support.

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Novel Mutations in X-Linked, USP26-Induced Asthenoteratozoospermia and Male Infertility

Cells ◽

10.3390/cells10071594 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1594

Author(s):

Chunyu Liu ◽

Ying Shen ◽

Qunshan Shen ◽

Wen Zhang ◽

Jiaxiong Wang ◽

...

Keyword(s):

Male Infertility ◽

Deubiquitinating Enzyme ◽

Linked Gene ◽

Real Time Quantitative Pcr ◽

Infertile Men ◽

Normal Sperm ◽

Abnormal Sperm ◽

Whole Exome ◽

Reproductive Counseling ◽

Hematoxylin Eosin

Male infertility is a multifactorial disease with a strong genetic background. Abnormal sperm morphologies have been found to be closely related to male infertility. Here, we conducted whole-exome sequencing in a cohort of 150 Han Chinese men with asthenoteratozoospermia. Two novel hemizygous mutations were identified in USP26, an X-linked gene preferentially expressed in the testis and encoding a deubiquitinating enzyme. These USP26 variants are extremely rare in human population genome databases and have been predicted to be deleterious by multiple bioinformatics tools. Hematoxylin-eosin staining and electron microscopy analyses of the spermatozoa from men harboring hemizygous USP26 variants showed a highly aberrant morphology and ultrastructure of the sperm heads and flagella. Real-time quantitative PCR and immunoblotting assays revealed obviously reduced levels of USP26 mRNA and protein in the spermatozoa from men harboring hemizygous deleterious variants of USP26. Furthermore, intracytoplasmic sperm injections performed on infertile men harboring hemizygous USP26 variants achieved satisfactory outcomes. Overall, our study demonstrates that USP26 is essential for normal sperm morphogenesis, and hemizygous USP26 mutations can induce X-linked asthenoteratozoospermia. These findings will provide effective guidance for the genetic and reproductive counseling of infertile men with asthenoteratozoospermia.

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