Real-time quantitative PCR-based serum neutralization test for detection and titration of neutralizing antibodies to chicken anemia virus

2004 ◽  
Vol 115 (2) ◽  
pp. 123-135 ◽  
Author(s):  
Vicky L van Santen ◽  
Bernhard Kaltenboeck ◽  
Kellye S Joiner ◽  
Kenneth S Macklin ◽  
Robert A Norton
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Neutralizing Antibodies ◽  
Neutralization Test ◽  
Chicken Anemia Virus ◽  
Serum Neutralization ◽  
Real Time Quantitative Pcr ◽  
Serum Neutralization Test ◽  
Anemia Virus

scholarly journals Simplified fluorescent inhibition microtest for the titration of rabies neutralizing antibodies

1993 ◽  
Vol 35 (2) ◽  
pp. 171-175 ◽  
Author(s):  
S.R. Favoretto ◽  
M.L. Carrieri ◽  
M.S. Tino ◽  
C.R. Zanetti ◽  
O.A.C. Pereira
Keyword(s):  
Neutralizing Antibodies ◽  
Neutralization Test ◽  
Inhibition Test ◽  
Mouse Serum ◽  
Enzymatic Assays ◽  
Serum Neutralization ◽  
Serum Neutralization Test ◽  
Pre Treatment ◽  
Fluorescent Focus

A simplified fluorescence inhibition microtest (SFIMT) was standardized for the evaluation of antirabies serum neutralizing antibodies based on the rapid fluorescent focus inhibition test (RFFIT) and the fluorescence inhibition microtest (FIMT). The simplified test showed reproductibility similar to that of the FIMT with advantages as easier executation and quicker reading. A simple pre-treatment of Brazilian microplates produced for immune enzymatic assays (PROSIL) gave equivalent results and substantial coast reduction, in relation to imported plates (DIFCO). The simplified test can be easily implemented in less sophisticated laboratories, as alternative to the mouse serum neutralization test, still the most largely employed in Brazil, or even to others as RFFIT and FIMT.

scholarly journals Quantification and Stability of Human Adenoviruses and Polyomavirus JCPyV in Wastewater Matrices

2006 ◽  
Vol 72 (12) ◽  
pp. 7894-7896 ◽  
Author(s):  
Silvia Bofill-Mas ◽  
Nestor Albinana-Gimenez ◽  
Pilar Clemente-Casares ◽  
Ayalkibet Hundesa ◽  
Jesus Rodriguez-Manzano ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
High Stability ◽  
Wastewater Sludge ◽  
Human Polyomavirus ◽  
Sewage Effluent ◽  
Viral Contamination ◽  
Human Adenoviruses ◽  
Urban Sewage ◽  
Real Time Quantitative Pcr

ABSTRACT Human adenoviruses (HAdV) and human polyomavirus JCPyV have been previously proposed as indicators of fecal viral contamination in the environment. Different wastewater matrices have been analyzed by applying real-time quantitative PCR procedures for the presence, quantity, and stability of a wide diversity of excreted HAdV and JCPyV. High quantities of HAdV and JCPyV were detected in sewage, effluent wastewater, sludge, and biosolid samples. Both viruses showed high stability in urban sewage. These results confirm the suitability of both viruses as indicators of human fecal viral pollution.

Analysis of Caecal Microbiota in Rats Fed with Genetically Modified Rice by Real-Time Quantitative PCR

2011 ◽  
Vol 76 (1) ◽  
pp. M88-M93 ◽  
Author(s):  
Wentao Xu ◽  
Liting Li ◽  
Jiao Lu ◽  
YunBo Luo ◽  
Ying Shang ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Genetically Modified ◽  
Genetically Modified Rice ◽  
Real Time Quantitative Pcr ◽  
Caecal Microbiota
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