Intra-laboratory validation of chronic bee paralysis virus quantitation using an accredited standardised real-time quantitative RT-PCR method

2012 ◽  
Vol 180 (1-2) ◽  
pp. 26-31 ◽  
Author(s):  
Philippe Blanchard ◽  
Julie Regnault ◽  
Frank Schurr ◽  
Eric Dubois ◽  
Magali Ribière
Keyword(s):  
Real Time ◽  
Rt Pcr ◽  
Pcr Method ◽  
Paralysis Virus

scholarly journals Trueness and precision of the real-time RT-PCR method for quantifying the chronic bee paralysis virus genome in bee homogenates evaluated by a comparative inter-laboratory study

2017 ◽  
Vol 248 ◽  
pp. 217-225 ◽  
Author(s):  
Frank Schurr ◽  
Nicolas Cougoule ◽  
Marie-Pierre Rivière ◽  
Magali Ribière-Chabert ◽  
Hamid Achour ◽  
...  
Keyword(s):  
Real Time ◽  
Laboratory Study ◽  
Virus Genome ◽  
Rt Pcr ◽  
The Real ◽  
Pcr Method ◽  
Paralysis Virus

scholarly journals Improved real-time RT-PCR method for high-throughput measurements using second derivative calculation and double correction

BioTechniques ◽  
2005 ◽  
Vol 38 (2) ◽  
pp. 287-293 ◽  
Author(s):  
Van Luu-The ◽  
Nathalie Paquet ◽  
Ezequiel Calvo ◽  
Jean Cumps
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Second Derivative ◽  
Rt Pcr ◽  
Pcr Method

Development of a one step real-time RT-PCR method for sensitive detection of human astrovirus

2006 ◽  
Vol 133 (1) ◽  
pp. 14-19 ◽  
Author(s):  
Enrique Royuela ◽  
Ana Negredo ◽  
Alicia Sánchez-Fauquier
Keyword(s):  
Real Time ◽  
Sensitive Detection ◽  
Rt Pcr ◽  
One Step ◽  
Human Astrovirus ◽  
Pcr Method

A NEW REAL-TIME RT-PCR. METHOD FOR THE DETECTION OF PSA POSITIVE CELLS IN BLOOD OF PROSTATE CANCER PATIENTS

2006 ◽  
Vol 5 (14) ◽  
pp. 788
Author(s):  
G. Barbero ◽  
P. Destefanis ◽  
S. Procida ◽  
C. Fiori ◽  
C. Ceruti ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Real Time ◽  
Cancer Patients ◽  
Rt Pcr ◽  
Pcr Method

scholarly journals A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR

2014 ◽  
Vol 64 (1) ◽  
pp. 81-92 ◽  
Author(s):  
Predrag Simeunović ◽  
Jevrosima Stevanović ◽  
Dejan Vidanović ◽  
Jakov Nišavić ◽  
Dejan Radović ◽  
...  
Keyword(s):  
Real Time ◽  
Honey Bee ◽  
Sampling Location ◽  
Rt Pcr ◽  
Deformed Wing Virus ◽  
Bee Colonies ◽  
Acute Bee Paralysis Virus ◽  
Paralysis Virus ◽  
Bee Viruses

Abstract In this study 55 honey bee colonies from different Serbian regions were monitored for the presence of Deformed Wing Virus (DWV) and Acute Bee Paralysis Virus (ABPV) using TaqMan-based real-time RT-PCR assay. The results revealed the presence of DWV in each sampling location, and ABPV in 10 out of 11 apiaries. High frequency of DWV (76.4%) and ABPV (61.8%) positive samples in asymptomatic colonies can be the consequence of inefficient and postponed Varroa treatment concerning the role of this mite in the transmission and activation of honey bee viruses. The real-time RTPCR technique described in this paper is proved to be the most reliable method for this kind of investigation.

scholarly journals Development of a real-time RT-PCR method for the detection of newly emerged highly pathogenic H7N9 influenza viruses

2017 ◽  
Vol 16 (9) ◽  
pp. 2055-2061 ◽  
Author(s):  
Xiu-rong WANG ◽  
Lin-lin GU ◽  
Jian-zhong SHI ◽  
Hai-feng XU ◽  
Ying ZHANG ◽  
...  
Keyword(s):  
Real Time ◽  
Influenza Viruses ◽  
Rt Pcr ◽  
Highly Pathogenic ◽  
Pcr Method ◽  
H7n9 Influenza

Absolute quantitation of RNA by a competitive real-time RT-PCR method using piscine nodavirus as a model

2006 ◽  
Vol 132 (1-2) ◽  
pp. 104-112 ◽  
Author(s):  
Søren Grove ◽  
Randi Faller ◽  
Karen Bækken Soleim ◽  
Birgit Helene Dannevig
Keyword(s):  
Real Time ◽  
Rt Pcr ◽  
Absolute Quantitation ◽  
Pcr Method

scholarly journals Relative expression ofPseudomonas aeruginosavirulence genes analyzed by a real time RT-PCR method during lung infection in rats

2005 ◽  
Vol 243 (1) ◽  
pp. 271-278 ◽  
Author(s):  
Joly Béatrice ◽  
Pierre Maud ◽  
Auvin Stéphane ◽  
Collet François ◽  
Gottrand Frédéric ◽  
...  
Keyword(s):  
Real Time ◽  
Lung Infection ◽  
Rt Pcr ◽  
Relative Expression ◽  
Pcr Method
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