Development and evaluation of real-time RT-PCR using ear hair for specific detection of sheep persistently infected with border disease virus (BDV)

2019 ◽  
Vol 269 ◽  
pp. 55-63
Author(s):  
Semmannan Kalaiyarasu ◽  
Niranjan Mishra ◽  
Katherukamem Rajukumar ◽  
Sthitha Pragnya Behera ◽  
Sandeep Kumar Jhade ◽  
...  
Keyword(s):  
Real Time ◽  
Disease Virus ◽  
Border Disease Virus ◽  
Rt Pcr ◽  
Specific Detection ◽  
Persistently Infected ◽  
Border Disease

scholarly journals Detection of Border Disease Virus in Fetuses, Stillbirths, and Newborn Lambs from Natural and Experimental Infections

2009 ◽  
Vol 21 (3) ◽  
pp. 331-337 ◽  
Author(s):  
Ana L. García-Pérez ◽  
Esmeralda Minguijón ◽  
Jesús F. Barandika ◽  
Gorka Aduriz ◽  
Inés Povedano ◽  
...  
Keyword(s):  
Disease Virus ◽  
Experimental Infection ◽  
Clinical Symptoms ◽  
Pcr Analysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Border Disease Virus ◽  
Rt Pcr ◽  
Genotype 4 ◽  
Antigen Elisa ◽  
Border Disease

The purpose of the present study was to evaluate the use of enzyme-linked immunosorbent assay (ELISA) antigen detection in blood or fetal fluids and reverse transcription polymerase chain reaction (RT-PCR) amplification in tissues for routine laboratory diagnosis of Border disease virus (BDV) infection. Samples from 67 fetuses, 6 stillbirths, and 11 lambs from 25 commercial flocks with suspicion of BDV abortion and 3 fetuses, 7 stillbirths, and 15 lambs obtained from an experimental infection with a local isolate (BDV genotype 4) were investigated. Presence of BDV was detected by RT-PCR in 7.9% of fetuses, 50% of stillbirths, and 50% of lambs from the commercial flocks analyzed, corresponding to 8 of the 25 farms (32%). A similar percentage of the lambs and stillbirths from the experimental infection were positive by RT-PCR of tissue samples (54.5%), and the highest positivity was detected in lymph node, thyroid gland, and kidney. The current study revealed that RT-PCR analysis of stillbirths and lambs with clinical symptoms is more suitable than the analysis of fetuses to confirm the presence of BDV in a flock. Pestiviral antigen was detected by antigen ELISA in a high proportion of fetuses (24/58) and stillbirths (3/4) from commercial flocks, but in lambs, the presence of colostral antibodies masked the detection of the antigen by ELISA. Nevertheless, in lambs from the experimental infection that were not fed colostrum, antigen ELISA was less efficient than RT-PCR in detecting viral presence in stillbirths and lambs. Antigen ELISA is therefore recommended for fetuses with advanced autolysis that can adversely affect RNA integrity.

scholarly journals Transmission of border disease virus from a persistently infected calf to seronegative heifers in early pregnancy

2015 ◽  
Vol 11 (1) ◽  
pp. 43 ◽  
Author(s):  
Ueli Braun ◽  
Monika Hilbe ◽  
Fredi Janett ◽  
Michael Hässig ◽  
Reto Zanoni ◽  
...  
Keyword(s):  
Disease Virus ◽  
Early Pregnancy ◽  
Border Disease Virus ◽  
Infected Calf ◽  
Persistently Infected ◽  
Border Disease

scholarly journals Molecular and serological investigation of border disease virus infection in sheep in the Kars District of Turkey

2014 ◽  
Vol 83 (3) ◽  
pp. 175-179 ◽  
Author(s):  
Volkan Yilmaz ◽  
Yakup Yildirim ◽  
Nuvit Coskun
Keyword(s):  
Nucleic Acid ◽  
Disease Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Small Scale ◽  
Border Disease Virus ◽  
Scale Production ◽  
Rt Pcr ◽  
Seropositivity Rate ◽  
Antigen Elisa ◽  
Border Disease

This study is a serological and virological examination of the border disease virus (BDV) in sheep at 1–5 years of age from private small scale production units of less than 20 sheep per unit, in the Kars District of Turkey. For this purpose, blood sera from 460 sheep were tested for antibodies against BDV using a commercial enzyme-linked immunosorbent assay (ELISA). Since BDV causes persistent infection, antigen-ELISA was also performed for this agent. Seropositivity rate was detected to be 74.57%. In addition, the BDV antigen was detected in one sample of seronegative sera (0.85%). Reverse transcription polymerase chain reaction (RT-PCR) technique was used to determine the presence of pestivirus nucleic acid by using 5’UTR primer pair. Pestivirus nucleic acid was found in 2 of 117 seronegative samples (1.71%) by RT-PCR. The results suggest that the infection was spreading in private small scale production units. Furthermore, recommendations for the control of BDV infection are presented. This study is the first molecular and serological study to determine viroprevalence and seroprevalence of BDV infection in sheep in the Kars District of Turkey.

scholarly journals Seroprevalence of Visna-Maedi Virus (VMV) and Border Disease Virus (BDV) in Van province and around

2018 ◽  
Vol 70 (4) ◽  
pp. 1029-1035
Author(s):  
P.S. Mohammad Ameen ◽  
Z. Karapınar
Keyword(s):  
Disease Virus ◽  
Border Disease Virus ◽  
Serum Samples ◽  
Persistently Infected ◽  
Economic Support ◽  
Border Disease

ABSTRACT The present study investigated the seroprevalance of Visna Maedi Virus (VMV) and Border Disease Virus (BDV) infections in sheeps in regions in and around Van province, Turkey. Sample materials were taken from 360 sheep sent to slaughterhouses around Van. All serum samples were examined using ELISA for antibodies for Visna Maedi (VMV) and Border Disease (BDV) viruses. Of these, 38 (10.5%) tested positive for Visna Maedi virus antibodies and 163 (45.2%) for Border Disease virus antibodies. Varying numbers of samples were positive for both virus antibodies across the towns of Erciş, Çaldıran, Erçek and Başkale in Van, Ağrı and Hakkari provinces. Both infections should be eliminated by informing veterinarians and animal owners, identifying and eliminating persistently infected animals from flocks, and conducting appropriate eradication measures. Economic support should be provided for this.

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