scholarly journals Development and validation of an IgM antibody capture ELISA for early detection of Hendra virus

2021 ◽  
pp. 114296
Author(s):  
Leanne McNabb ◽  
Alicia Andiani ◽  
Aiste Bulavaite ◽  
Aurelija Zvirbliene ◽  
Kestutis Sasnauskas ◽  
...  
Keyword(s):  
Early Detection ◽  
Hendra Virus ◽  
Capture Elisa ◽  
Igm Antibody ◽  
Antibody Capture ◽  
Development And Validation

The Development and Interlaboratory Validation of a Quantitative Antibody Capture ELISA for the Measurement of Specific Guinea-pig IgG1: An Alternative to the Passive Cutaneous Anaphylaxis Assay

1996 ◽  
Vol 24 (1) ◽  
pp. 73-79
Author(s):  
Laura S. Babcock ◽  
Thomas T. Kawabata ◽  
Victor S. Moore ◽  
Catherine M. Condo ◽  
Annette Prentø
Keyword(s):  
Guinea Pig ◽  
Passive Cutaneous Anaphylaxis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Antigens ◽  
Capture Elisa ◽  
Elisa Format ◽  
Interlaboratory Validation ◽  
Antibody Capture ◽  
Development And Validation

Specific guinea-pig IgG1 has traditionally been measured by using an in vivo guinea-pig passive cutaneous anaphylaxis (PCA) assay. This paper describes the development and validation of a quantitative enzyme-linked immunosorbent assay (ELISA) for specific IgG1 against two protein antigens (Alcalase and Enzyme B) as an alternative to the PCA assay. The ELISA format involved a rabbit antibody bound to microtitre plates to capture the antigen. The test sera is added to this, followed sequentially by goat anti-guinea-pig IgG1 and rabbit anti-goat-IgG-alkaline phosphatase conjugate. Aliquots of each serum sample from immunised guinea-pigs were analysed with the ELISA for specific IgG1 titres in three laboratories and were compared to titres determined by using the PCA assay. The findings demonstrate that there is a good correlation between the ELISA and the PCA assay and that the ELISA shows good interlaboratory reproducibility. Thus, the antibody capture ELISA described in this report is a valid and robust replacement for the guinea-pig PCA assay.

Validation of an IgM antibody capture ELISA based on a recombinant nucleoprotein for identification of domestic ruminants infected with Rift Valley fever virus

2011 ◽  
Vol 177 (2) ◽  
pp. 140-146 ◽  
Author(s):  
Roy Williams ◽  
Charlotte Elizabeth Ellis ◽  
Shirley Jacqueline Smith ◽  
Christiaan Abraham Potgieter ◽  
David Wallace ◽  
...  
Keyword(s):  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever ◽  
Capture Elisa ◽  
Igm Antibody ◽  
Domestic Ruminants ◽  
Antibody Capture

scholarly journals Red-cell IgM-antibody capture assay for the detection of Mycoplasma pneumoniae-specific IgM

1988 ◽  
Vol 100 (1) ◽  
pp. 101-109 ◽  
Author(s):  
R. R. A. Coombs ◽  
Gwen Easter ◽  
P. Matejtschuk ◽  
T. G. Wreghitt
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Rapid Test ◽  
Red Cells ◽  
Red Cell ◽  
Similar Time ◽  
Capture Elisa ◽  
Test Taking ◽  
Igm Antibody ◽  
Capture Assay ◽  
Antibody Capture

SUMMARYA red-cell IgM-antibody capture assay has been developed for detecting Mycoplasma pneumoniae-specific IgM, which is based on the adsorption or ‘capture’ of IgM from patients' sera onto so-called ‘inagglutinable’ bovine red cells, chemically linked with anti-human μ When M. pneumoniae antigen is added to the system, the red cells agglutinate in the presence of M. pneumoniae-specific IgM.The test was compared with the μ-capture ELISA described by Wreghitt & Sillis (1985), and was found to give comparable results. The two tests had similar sensitivity and specificity and could detect M. pneumoniae-spcific IgM for a similar time (up to 6 months) after proven M. pneumoniae infection.However, the red-cell antibody capture assay is a much more simple and rapid test, taking only 1 h to perform (compared to 24 h for μ-capture ELISA). The redcell IgM-antibody capture assay is therefore amenable to rapid diagnosis of M. pneumoniae infection and the institution of early appropriate antibiotic therapy.

scholarly journals Capacity of a Multiplex IgM Antibody Capture ELISA to Differentiate Zika and Dengue Virus Infections in Areas of Concurrent Endemic Transmission

2021 ◽  
Author(s):  
Freddy A. Medina ◽  
Frances Vila ◽  
Lakshmanane Premkumar ◽  
Olga Lorenzi ◽  
Gabriela Paz-Bailey ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Cross Reactivity ◽  
Virus Infections ◽  
Rt Pcr ◽  
Capture Elisa ◽  
Igm Antibody ◽  
Antibody Capture ◽  
Polymerase Chain ◽  
Endemic Transmission ◽  
Post Onset

Serological cross-reactivity has proved to be a challenge to diagnose Zika virus (ZIKV) infections in dengue virus (DENV) endemic countries. Confirmatory testing of ZIKV IgM positive results by plaque reduction neutralization tests (PRNTs) provides clarification in only a minority of cases because most individuals infected with ZIKV were previously exposed to DENV. The goal of this study was to evaluate the performance of a ZIKV/DENV DUO IgM antibody capture ELISA (MAC-ELISA) for discriminating between DENV and ZIKV infections in endemic regions. Our performance evaluation included acute and convalescent specimens from patients with real-time reverse transcription polymerase chain reaction (RT-PCR)-confirmed DENV or ZIKV from the Sentinel Enhanced Dengue Surveillance System in Ponce, Puerto Rico. The ZIKV/DENV DUO MAC-ELISA specificity was 100% for DENV (N = 127) and 98.4% for ZIKV (N = 275) when specimens were tested during the optimal testing window (days post-onset of illness [DPO] 6–120). The ZIKV/DENV DUO MAC-ELISA sensitivity of RT-PCR confirmed specimens reached 100% for DENV by DPO 6 and for ZIKV by DPO 9. Our new ZIKV/DENV DUO MAC-ELISA was also able to distinguish ZIKV and DENV regardless of previous DENV exposure. We conclude this novel serologic diagnostic assay can accurately discriminate ZIKV and DENV infections. This can potentially be useful considering that the more labor-intensive and expensive PRNT assay may not be an option for confirmatory diagnosis in areas that lack PRNT capacity, but experience circulation of both DENV and ZIKV.

Development and evaluation of a capture ELISA for IgM antibody to the human cytomegalovirus major DNA binding protein

1991 ◽  
Vol 35 (3) ◽  
pp. 315-329 ◽  
Author(s):  
M.Grazia Revello ◽  
Elena Percivalle ◽  
Marco Zannino ◽  
Valdano Rossi ◽  
Giuseppe Gerna
Keyword(s):  
Dna Binding ◽  
Human Cytomegalovirus ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Capture Elisa ◽  
Igm Antibody

scholarly journals Comparison of Seven Commercial Antigen and Antibody Enzyme-Linked Immunosorbent Assays for Detection of Acute Dengue Infection

2012 ◽  
Vol 19 (5) ◽  
pp. 804-810 ◽  
Author(s):  
Stuart D. Blacksell ◽  
Richard G. Jarman ◽  
Robert V. Gibbons ◽  
Ampai Tanganuchitcharnchai ◽  
Mammen P. Mammen ◽  
...  
Keyword(s):  
South Korea ◽  
Dengue Virus ◽  
Dengue Infection ◽  
Virus Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Capture Elisa ◽  
Igm Antibody ◽  
Antigen Elisa ◽  
Ns1 Antigen ◽  
Elisa Format

ABSTRACTSeven commercial assays were evaluated to determine their suitability for the diagnosis of acute dengue infection: (i) the Panbio dengue virus Pan-E NS1 early enzyme-linked immunosorbent assay (ELISA), second generation (Alere, Australia); (ii) the Panbio dengue virus IgM capture ELISA (Alere, Australia); (iii) the Panbio dengue virus IgG capture ELISA (Alere, Australia); (iv) the Standard Diagnostics dengue virus NS1 antigen ELISA (Standard Diagnostics, South Korea); (v) the Standard Diagnostics dengue virus IgM ELISA (Standard Diagnostics, South Korea); (vi) the Standard Diagnostics dengue virus IgG ELISA (Standard Diagnostics, South Korea); and (vii) the Platelia NS1 antigen ELISA (Bio-Rad, France). Samples from 239 Thai patients confirmed to be dengue virus positive and 98 Sri Lankan patients negative for dengue virus infection were tested. The sensitivities and specificities of the NS1 antigen ELISAs ranged from 45 to 57% and 93 to 100% and those of the IgM antibody ELISAs ranged from 85 to 89% and 88 to 100%, respectively. Combining the NS1 antigen and IgM antibody results from the Standard Diagnostics ELISAs gave the best compromise between sensitivity and specificity (87 and 96%, respectively), as well as providing the best sensitivity for patients presenting at different times after fever onset. The Panbio IgG capture ELISA correctly classified 67% of secondary dengue infection cases. This study provides strong evidence of the value of combining dengue virus antigen- and antibody-based test results in the ELISA format for the diagnosis of acute dengue infection.

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