Blood storage at 4°C—factors involved in DNA yield and quality

2006 ◽  
Vol 147 (6) ◽  
pp. 290-294 ◽  
Author(s):  
Andrea J. Richardson ◽  
Niro Narendran ◽  
Robyn H. Guymer ◽  
Hien Vu ◽  
Paul N. Baird
Keyword(s):  
Blood Storage ◽  
Dna Yield ◽  
Yield And Quality

High Microsatellite and SNP Genotyping Success Rates Established in a Large Number of Genomic DNA Samples Extracted From Mouth Swabs and Genotypes

2006 ◽  
Vol 9 (4) ◽  
pp. 501-506 ◽  
Author(s):  
Josine L. Min ◽  
Nico Lakenberg ◽  
Margreet Bakker-Verweij ◽  
Eka Suchiman ◽  
Dorret I. Boomsma ◽  
...  
Keyword(s):  
Success Rate ◽  
Genomic Dna ◽  
Snp Genotyping ◽  
High Yield ◽  
Success Rates ◽  
Nucleotide Polymorphism ◽  
High Quality ◽  
Single Nucleotide ◽  
Dna Yield ◽  
Yield And Quality

AbstractIn this article, we present the genomic DNA yield and the microsatellite and single nucleotide polymorphism (SNP) genotyping success rates of genomic DNA extracted from a large number of mouth swab samples. In total, the median yield and quality was determined in 714 individuals and the success rates in 378,480 genotypings of 915 individuals. The median yield of genomic DNA per mouth swab was 4.1 μg (range 0.1–42.2 μg) and was not reduced when mouth swabs were stored for at least 21 months prior to extraction. A maximum of 20 mouth swabs is collected per participant. Mouth swab samples showed in, respectively, 89% for 390 microsatellites and 99% for 24 SNPs a genotyping success rate higher than 75%. A very low success rate of genotyping (0%–10%) was obtained for 3.2% of the 915 mouth swab samples using microsatellite markers. Only 0.005% of the mouth swab samples showed a geno-typing success rate lower than 75% (range 58%–71%) using SNPs. Our results show that mouth swabs can be easily collected, stored by our conditions for months prior to DNA extraction and result in high yield and high-quality DNA appropriate for genotyping with high success rate including whole genome searches using microsatellites or SNPs.

scholarly journals Antifreeze Solution Improves DNA Recovery by Preserving the Integrity of Pathogen-Infected Blood and Other Tissues

2000 ◽  
Vol 7 (6) ◽  
pp. 945-946 ◽  
Author(s):  
Diana Sara Leal-Klevezas ◽  
Irma Olivia Martı́nez-Vázquez ◽  
Baltazar Cuevas-Hernández ◽  
Juan Pablo Martı́nez-Soriano
Keyword(s):  
Dna Extraction ◽  
Freeze Drying ◽  
Microscopic Analysis ◽  
Infected Blood ◽  
Dna Recovery ◽  
Dna Yield ◽  
Yield And Quality ◽  
Field Samples ◽  
Protease Treatment ◽  
Novel Method

ABSTRACT Preserving blood samples for shipping and later DNA extraction has been performed by cooling, freezing, drying, freeze-drying, and protease treatment, among other methods. Most methods to preserve field samples for further DNA extraction do not prevent cellular and DNA damage or are useful only in preserving them for short periods. This report introduces a novel method for blood and tissue that allows preservation in freezing temperatures for a prolonged period of time. The solution reported here (20% ethylene glycol-propylene glycol) preserves cells and tissues integrity, as judged by microscopic analysis, and improves DNA yield and quality.

scholarly journals Determinants of DNA yield and quality from different non-invasive sampling methods

2012 ◽  
Vol 19 (2) ◽  
pp. 56-61
Author(s):  
Y.F. Choon ◽  
L.P. Karen-Ng ◽  
S. Hassan ◽  
J. Marhazlinda ◽  
R.B. Zain
Keyword(s):  
Sampling Methods ◽  
Dna Yield ◽  
Non Invasive ◽  
Yield And Quality

scholarly journals DNA Cards: Determinants of DNA Yield and Quality in Collecting Genetic Samples for Pharmacogenetic Studies

2007 ◽  
Vol 101 (2) ◽  
pp. 132-137 ◽  
Author(s):  
Sergi Mas ◽  
Anna Crescenti ◽  
Patricia Gassó ◽  
Jose M Vidal-Taboada ◽  
Amalia Lafuente
Keyword(s):  
Dna Yield ◽  
Yield And Quality

ANALYSIS OF FOUR DIFFERENT METHODS OF DNA EXTRACTION FROM THE TRUE WEEVIL (COLEOPTERA: CURCULIONIDAE)

2018 ◽  
Vol 24 (1) ◽  
Author(s):  
DIVYA SHARMA ◽  
DALIP KUMAR ◽  
RHITOBAN RAY CHOUDHURY
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Extraction Method ◽  
Sitophilus Oryzae ◽  
Potassium Acetate ◽  
Single Specimen ◽  
High Quality ◽  
Dna Yield ◽  
Yield And Quality ◽  
Dna Extraction Method

PCR-based markers have been widely used for the analysis of genetic diversity and to avoid ambiguity, molecular characterization is very effective tool for accurate discrimination and identification of a species in insects. Because these studies require analysis of large number of samples, a DNA extraction method that is fast, inexpensive and yields high quality DNA from the preserved samples, needs to be evaluated. A comparative analysis of four methods for DNA extraction from a single specimen of rice weevil, Sitophilus oryzae preserved in 90% alcohol has been communicated. Significantly higher DNA yields were obtained by using SDS-Potassium acetate method followed by CTAB, DNA XPress and Bioline Isolate II genomic DNA kit. Maximum purity (A260/A280- 1.8) was obtained with Bioline Isolate II genomic DNA kit method. The Absorbance ratio was appreciably low with DNA Xpress kit showing the presence of proteins. Bioline Isolate II genomic DNA kit was time efficient and yielded good quality DNA but at a high cost. Based on DNA yield and quality, these evaluations provide a guide for choosing Bioline Isolate II genomic DNA kit method of DNA extraction for rice weevils and optimizing the extraction conditions for rice weevils.

scholarly journals Paramagnetic Cellulose DNA Isolation Improves DNA Yield and Quality Among Diverse Plant Taxa

2014 ◽  
Vol 2 (10) ◽  
pp. 1400048 ◽  
Author(s):  
Jackson R. Moeller ◽  
Nicholas R. Moehn ◽  
Donald M. Waller ◽  
Thomas J. Givnish
Keyword(s):  
Dna Isolation ◽  
Dna Yield ◽  
Yield And Quality ◽  
Diverse Plant

scholarly journals DNA yield and quality of saliva samples and suitability for large-scale epidemiological studies in children

2011 ◽  
Vol 35 (S1) ◽  
pp. S113-S118 ◽  
Author(s):  
A C Koni ◽  
◽  
R A Scott ◽  
G Wang ◽  
M E S Bailey ◽  
...  
Keyword(s):  
Large Scale ◽  
Epidemiological Studies ◽  
Dna Yield ◽  
Yield And Quality
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