scholarly journals The therapeutic effect of CD133+ cells derived from human umbilical cord blood on neonatal mouse hypoxic-ischemic encephalopathy model

Life Sciences ◽  
2016 ◽  
Vol 157 ◽  
pp. 108-115 ◽  
Author(s):  
Yukie Kidani ◽  
Yasuo Miki ◽  
Nana Nomimura ◽  
Shiori Minakawa ◽  
Norifumi Tanaka ◽  
...  
Keyword(s):  
Cord Blood ◽  
Therapeutic Effect ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Neonatal Mouse ◽  
Hypoxic Ischemic Encephalopathy ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Ischemic Encephalopathy

Therapeutic effect of human umbilical cord blood cells on diebetic mice

2008 ◽  
Vol 32 (3) ◽  
pp. S25-S25
Author(s):  
Fang Ting Zhang ◽  
Wan Hui Juan ◽  
Jing Ye ◽  
Jie Yu ◽  
Xia Long ◽  
...  
Keyword(s):  
Cord Blood ◽  
Therapeutic Effect ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Cord Blood Cells ◽  
Umbilical Cord Blood Cells

Evaluations of Intravenous Administration of CD34+ Human Umbilical Cord Blood Cells in a Mouse Model of Neonatal Hypoxic-Ischemic Encephalopathy

2016 ◽  
Vol 38 (5) ◽  
pp. 331-341 ◽  
Author(s):  
Makiko Ohshima ◽  
Akihiko Taguchi ◽  
Yoshiaki Sato ◽  
Yuko Ogawa ◽  
Satoshi Saito ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Intravenous Administration ◽  
Umbilical Cord Blood ◽  
Treatment Protocol ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Therapeutic Effects ◽  
Neonatal Encephalopathy ◽  
Ischemic Encephalopathy

Several cell therapies have been explored as novel therapeutic strategies for neonatal encephalopathy because the benefits of current treatments are limited. We previously reported that intravenous administration of human umbilical cord blood (hUCB) CD34+ cells (hematopoietic stem cells/endothelial progenitor cells) at 48 h after insult exerts therapeutic effects in neonatal mice with stroke, i.e., permanent middle cerebral artery occlusion. Although neonatal stroke and hypoxic-ischemic encephalopathy (HIE) are grouped under the term “neonatal encephalopathy,” their pathogenesis differs. However, little is known about the differences in the effects of the same treatment between these 2 diseases. In this study, we investigated whether the same treatment protocol exerts therapeutic effects in neonatal mice with HIE. The treatment significantly ameliorated the decreased cerebral blood flow in the ischemic penumbra. Although the cylinder and rotarod tests showed a trend of amelioration of behavioral impairments from the treatment, these were not statistically significant. Morphological brain injuries were not altered by treatment. The cell administration did not cause any adverse effects apart from hyperactivity in the open-field test. Some of these findings are consistent with the results obtained in our previous study using a stroke model, but others are not. This study suggests that the treatment protocol needs to be optimized for each pathological condition.

scholarly journals Synergistic Effect in Neurological Recovery via Anti-Apoptotic Akt Signaling in Umbilical Cord Blood and Erythropoietin Combination Therapy for Neonatal Hypoxic-Ischemic Brain Injury

2021 ◽  
Vol 22 (21) ◽  
pp. 11995
Author(s):  
Jee In Choi ◽  
Joo-Wan Choi ◽  
Kyu-Ho Shim ◽  
Jin Seung Choung ◽  
Hyun-Jin Kim ◽  
...  
Keyword(s):  
Cerebral Palsy ◽  
Combination Therapy ◽  
Cord Blood ◽  
Therapeutic Effect ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Therapy Group ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Akt Inhibitor

Our previous clinical studies demonstrated the synergistic therapeutic effect induced by co-administering recombinant human erythropoietin (rhEPO) in human umbilical cord blood (hUCB) therapy for children with cerebral palsy. However, the cellular mechanism beyond the beneficial effects in this combination therapy still needs to be elucidated. A hypoxic–ischemic encephalopathy (HIE) model of neonates, representing cerebral palsy, was prepared and randomly divided into five groups (hUCB+rhEPO combination, hUCB, and rhEPO treatments over HIE, HIE control, and sham). Seven days after, hUCB was administered intraperitoneally and the rhEPO injections were started. Neurobehavioral tests showed the best outcome in the combination therapy group, while the hUCB and rhEPO alone treatments also showed better outcomes compared with the control (p < 0.05). Inflammatory cytokines were downregulated by the treatments and attenuated most by the combination therapy (p < 0.05). The hUCB+rhEPO treatment also showed remarkable increase in phosphorylation of Akt and potentiation of anti-apoptotic responses with decreased Bax and increased Bcl-2 (p < 0.05). Pre-treatment of MK-2206, an Akt inhibitor, for the combination therapy depressed the anti-apoptotic effects. In conclusion, these findings suggest that the therapeutic effect of hUCB therapy might be potentiated by co-administration of rhEPO via augmentation of anti-inflammatory and anti-apoptotic responses related to the phosphorylation of Akt.

scholarly journals Therapeutic effect of human umbilical cord blood cells on diebetic mice

Cell Research ◽  
2008 ◽  
Vol 18 (S1) ◽  
pp. S48-S48
Author(s):  
Fang-ting Zhang ◽  
Hui-juan Wan ◽  
Jing Ye ◽  
Jie Yu ◽  
Xia Long ◽  
...  
Keyword(s):  
Cord Blood ◽  
Therapeutic Effect ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Cord Blood Cells ◽  
Umbilical Cord Blood Cells

scholarly journals Peptidomic Analysis of Neonate Umbilical Cord Blood for the Identification of Endogenous Peptides Involved in Hypoxic–Ischemic Encephalopathy

2021 ◽  
Vol 9 ◽  
Author(s):  
Xiaohua Dong ◽  
Jing Zhao ◽  
Yinyin Shen ◽  
Qing Sun ◽  
Xiaohui Wu ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Differentially Expressed ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Endogenous Peptides ◽  
Precursor Proteins ◽  
Ischemic Encephalopathy ◽  
Normal Controls

Neonatal hypoxic–ischemic encephalopathy (HIE) is a common neurological disorder triggered by perinatal cerebral ischemia and hypoxia. Accumulating evidence has shown that peptides have neuroprotective effects in nerve injury. However, the function of endogenous peptides in the pathogenesis of HIE has not been studied. In the present study, a comparative peptidomic profile was performed in the serum of the human umbilical cord blood with HIE (three patients) and the control group (three health control) by liquid chromatography–mass spectrometry (LC-MS). Our study demonstrated that a total of 49 peptides derived from 25 precursor proteins were differentially expressed in the serum of HIE compared with normal controls, including 33 upregulated peptides and 16 downregulated peptides. Each of the differentially expressed peptides has specific characteristics, including pI, Mw, and cleavage pattern. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that the precursor proteins of differentially expressed peptides participate in the different biological process. Moreover, among the 49 differentially expressed peptides, 21 peptides were identified from the fibrinogen chain family, which plays a role in neurological diseases, suggesting that these peptides may play an important role in maintaining brain health. In conclusion, our results showed a comparative peptidomic profile from human umbilical cord blood of HIE patients and normal controls. These dysregulated peptides may have potentially important functions in umbilical cord blood with HIE and may be involved in the pathogenesis of the HIE.

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