Alpha-tocopherol succinate increases cyclooxygenase-2 activity: Tissue-specific action in pregnant rat uterus in vitro

Life Sciences ◽  
2018 ◽  
Vol 192 ◽  
pp. 199-204 ◽  
Author(s):  
Anna Kothencz ◽  
Judit Hajagos-Tóth ◽  
Adrienn Csányi ◽  
Róbert Gáspár
Keyword(s):  
Cyclooxygenase 2 ◽  
Alpha Tocopherol ◽  
Rat Uterus ◽  
Pregnant Rat ◽  
Tissue Specific

EVIDENCE FOR A POSSIBLE FOETAL CONTROL OF PROSTAGLANDIN RELEASE FROM THE PREGNANT RAT UTERUS IN VITRO

1975 ◽  
Vol 65 (3) ◽  
pp. 429-437 ◽  
Author(s):  
M. J. PARNHAM ◽  
J. M. SNEDDON ◽  
K. I. WILLIAMS
Keyword(s):  
Arachidonic Acid ◽  
Uterine Horn ◽  
Rat Uterus ◽  
Pregnant Rat ◽  
Uterine Activity ◽  
Prostaglandin Release ◽  
Prostaglandin F ◽  
Spontaneous Contractions ◽  
Stimulation Of

SUMMARY The release of prostaglandin-like material and the spontaneous contractions of individual horns from the pregnant rat uterus in vitro have been studied on day 22 of pregnancy – the expected day of delivery. Removal of foetuses (retaining placentae in utero) from one or both uterine horns on day 16 or 17 significantly reduced prostaglandin F release and spontaneous activity. Rats which had been made unilaterally pregnant after ligation of one uterine horn, exhibited a decrease in prostaglandin F output from both horns. Uterine activity and prostaglandin release were increased in quiescent uteri by the addition of arachidonic acid (5 μg/ml) or phospholipase A (160 mu./ml); these effects were abolished by indomethacin (20 μg/ml). However, the stimulation of uterine activity by PGF2α (30–60 ng/ml) was not affected by indomethacin. It is concluded that the release of prostaglandins from the pregnant rat uterus in vitro at term is related to the presence of viable foetuses.

scholarly journals GRANULATION TISSUE AS A CONTRACTILE ORGAN

1972 ◽  
Vol 135 (4) ◽  
pp. 719-734 ◽  
Author(s):  
G. Gabbiani ◽  
B. J. Hirschel ◽  
G. B. Ryan ◽  
P. R. Statkov ◽  
G. Majno
Keyword(s):  
Smooth Muscle ◽  
Connective Tissue ◽  
Granulation Tissue ◽  
Adenosine Triphosphatase ◽  
Cell Type ◽  
Rat Uterus ◽  
Pregnant Rat ◽  
Tissue Contraction ◽  
A Cell

Contracting granulation tissues contain fibroblasts that develop characteristics typical of smooth muscle: (a) They contain an extensive cytoplasmic fibrillar system. (b) They show immunofluorescent labeling of their cytoplasm with human anti-smooth muscle serum. (c) The nuclei show complicated folds and indentations, indicative of cellular contraction. (d) There are cell-to-cell and cell-to-stroma attachments. (e) It is possible to extract similar quantities of actomyosin (having the same adenosine triphosphatase activity) from granulation tissue and from pregnant rat uterus. (f) Strips of granulation tissue, when tested pharmacologically in vitro, behave similarly to smooth muscle. All these data support the view that, under certain conditions, fibroblasts can differentiate into a cell type structurally and functionally similar to smooth muscle and that this cell, the "myo-fibroblast," plays an important role in connective tissue contraction.

THE INFLUENCE OF OVARIAN HORMONES UPON THE MOTILITY AND PROSTAGLANDIN PRODUCTION OF THE PREGNANT RAT UTERUS IN VITRO

1974 ◽  
Vol 60 (2) ◽  
pp. 343-351 ◽  
Author(s):  
P. J. HARNEY ◽  
J. M. SNEDDON ◽  
K. I. WILLIAMS
Keyword(s):  
Spontaneous Activity ◽  
Post Partum ◽  
Ethyl Oleate ◽  
Control Group ◽  
Rat Uterus ◽  
Pregnant Rat ◽  
Uterine Activity ◽  
Prostaglandin Release ◽  
Spontaneous Contractions

SUMMARY The output of prostaglandin-like material and the spontaneous contractions of the pregnant rat uterus in vitro have been studied during the last 6 days of pregnancy and for 3 days post partum. Both prostaglandin release and uterine activity were minimal on days 17–18 of pregnancy but both parameters gradually increased, reaching a peak on day 22, the expected day of delivery. Post partum both uterine prostaglandin release and spontaneous activity declined. Progesterone (25 mg, i.m.) given to rats from days 16–21 of pregnancy did not alter uterine activity or prostaglandin output when compared with uteri taken on day 22 from animals which had received ethyl oleate over the same period. On day 22 the spontaneous activity of uteri in vitro taken from animals ovariectomized on day 17 was very low compared with that seen in preparations from sham-operated controls, although prostaglandin release in these groups was not significantly different. Oestrogen (1 μg, i.m.) was given to one group of ovariectomized animals on days 19 and 20; uterine activity was determined on day 21 of pregnancy and found to be of greater intensity and amplitude than that seen in an ovariectomized control group. Prostaglandin output was similar in these groups. Thus although exogenous progesterone and oestrogen do not influence uterine prostaglandin release at term, oestrogen appears essential for the occurrence of spontaneous contractions. It is concluded that, in the pregnant rat uterus in vitro, prostaglandin release may contribute to uterine activity but oestrogen is essential for this to become apparent.

Immunohistochemical Localization of Cyclooxygenase-2 in Pregnant Rat Uterus by Sp-6 Acupuncture

2003 ◽  
Vol 31 (03) ◽  
pp. 481-488 ◽  
Author(s):  
Jeong-Sang Kim ◽  
Chang Su Na ◽  
Woo Jun Hwang ◽  
Byung Chul Lee ◽  
Ki Hyoung Shin ◽  
...  
Keyword(s):  
Acupuncture Treatment ◽  
Immunohistochemical Localization ◽  
Cyclooxygenase 2 ◽  
Uterine Contractility ◽  
Rat Uterus ◽  
Pregnant Rats ◽  
Pregnant Rat ◽  
Complementary Method ◽  
Key Factor ◽  
Cox 2

As pregnancy advances, prostaglandins (PG) increase in the uterus, leading to elevated uterine contractility. Therefore, regulating the concentration of PG in the uterus can be a key factor for controlling the duration of labor. Since the synthesis of PGs in the uterus is catalyzed by cyclooxygenase-2 (COX-2), devising a tool to regulate the expression of COX-2 could provide a method for treating complicated labor. In this study, Sp-6 acupuncture treatment was evaluated for its potential in controlling uterine motility. Immunohistochemical methods showed the COX-2 enzyme was primarily found in the endometrium and myometrium of rat uterus. COX-2 expression in these two locations were intensified by pregnancy, but reduced by acupuncture at the Sp-6 acupoint. Uterine motility monitored during Sp-6 acupuncture was reduced by 28.15% (p < 0.05) and 19.88% (p < 0.05) in pregnant rats and non-pregnant rats, respectively. The significant reduction of uterine motility in pregnant rat suggests a role for Sp-6 acupuncture in regulating the expression of COX-2 during pregnancy. These results suggest that Sp-6 acupuncture could be used as a complementary method for controlling labor in human pregnancy.

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