Interactive effects of nutrition, reproductive state and pollution on molecular stress responses of mussels, Mytilus galloprovincialis Lamarck, 1819

2017 ◽  
Vol 131 ◽  
pp. 103-115 ◽  
Author(s):  
Carmen González-Fernández ◽  
Marina Albentosa ◽  
Inna Sokolova
Keyword(s):  
Stress Responses ◽  
Mytilus Galloprovincialis ◽  
Interactive Effects ◽  
Reproductive State

scholarly journals Post-ejaculation thermal stress causes changes to the RNA profile of sperm in an external fertilizer

2020 ◽  
Vol 287 (1938) ◽  
pp. 20202147
Author(s):  
Rowan A. Lymbery ◽  
Jonathan P. Evans ◽  
W. Jason Kennington
Keyword(s):  
Stress Responses ◽  
Messenger Rna ◽  
Mytilus Galloprovincialis ◽  
Target Genes ◽  
Sperm Function ◽  
Mrna Levels ◽  
Embryo Viability ◽  
Paired Samples ◽  
Degradation Rates ◽  
Highly Sensitive

Sperm cells experience considerable post-ejaculation environmental variation. However, little is known about whether this affects their molecular composition, probably owing to the assumption that sperm are transcriptionally quiescent. Nevertheless, recent evidence shows sperm have distinct RNA profiles that affect fertilization and embryo viability. Moreover, RNAs are expected to be highly sensitive to extracellular changes. One such group of RNAs are heat shock protein (hsp) transcripts, which function in stress responses and are enriched in sperm. Here, we exploit the experimental tractability of the mussel Mytilus galloprovincialis by exposing paired samples of ejaculated sperm to ambient (19°C) and increased (25°C) temperatures, then measure (i) sperm motility phenotypes, and (ii) messenger RNA (mRNA) levels of two target genes ( hsp70 and hsp90 ) and several putative reference genes. We find no phenotypic changes in motility, but reduced mRNA levels for hsp90 and the putative reference gene gapdh at 25°C. This could reflect either decay of specific RNAs, or changes in translation and degradation rates of transcripts to maintain sperm function under stress. These findings represent, to our knowledge, the first evidence for changes in sperm RNA profiles owing to post-ejaculation environments, and suggest that sperm may be more vulnerable to stress from rising temperatures than currently thought.

Interactive effects of n-TiO2 and 2,3,7,8-TCDD on the marine bivalve Mytilus galloprovincialis

2014 ◽  
Vol 153 ◽  
pp. 53-65 ◽  
Author(s):  
Laura Canesi ◽  
Giada Frenzilli ◽  
Teresa Balbi ◽  
Margherita Bernardeschi ◽  
Caterina Ciacci ◽  
...  
Keyword(s):  
Mytilus Galloprovincialis ◽  
Interactive Effects ◽  
Marine Bivalve

scholarly journals Stress: conceptualization, framing, and induction

2021 ◽  
Author(s):  
Jenny Jing Wen Liu
Keyword(s):  
Stress Responses ◽  
Stress Reactivity ◽  
Past Research ◽  
Task Demands ◽  
Interactive Effects ◽  
Negative Events ◽  
Differential Stress ◽  
Stress Induction ◽  
Subjective Perceptions ◽  
Stress Study

Past research has often conceptualized stress from a deficit-oriented approach. This approach is unbalanced, often associating stress with negative events and outcomes. The current study examined stress from the Transactional Model, and proposed alternative ways to conceptualize stress. In addition, both psychosocial and physiological stressors were utilized to induce stress. Study aims were threefold: (1) to examine beliefs about stress and effects of framing on changes to perceptions of stress, (2) to examine the comparative effects of two different stress-induction methods, and (3) to explore the interactive effects of framing and stress-induction on subjective perceptions of stress and measures of stress reactivity. Results confirmed a deficit-orientation of stress within the sampled population. Comparative effects of both stressors highlighted differential stress responses based on task demands and appraisal. Finally, interactive effects of framing and stress-induction provided support for alternative conceptualizations of stress in adaptive coping.

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