Mineralized nodule formation in primary osteoblasts culture in titanium doped phosphate glass and in-house prepared freeze dried demineralized bone extracts

2021 ◽  
pp. 125425
Author(s):  
Ensanya A. Abou Neel ◽  
Aghila Rani KG ◽  
A.R. Samsudin
Keyword(s):  
Phosphate Glass ◽  
Nodule Formation ◽  
Freeze Dried ◽  
Primary Osteoblasts ◽  
Demineralized Bone

scholarly journals Comparative Effectiveness of Bone Grafting Using Xenograft Freeze-Dried Cortical Bovine, Allograft Freeze-Dried Cortical New Zealand White Rabbit, Xenograft Hydroxyapatite Bovine, and Xenograft Demineralized Bone Matrix Bovine in Bone Defect of Femoral Diaphysis of White Rabbit: Experimental Study In Vivo

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Ferdiansyah Mahyudin ◽  
Dwikora Novembri Utomo ◽  
Heri Suroto ◽  
Tri Wahyu Martanto ◽  
Mouli Edward ◽  
...  
Keyword(s):  
New Zealand ◽  
Bone Defect ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Zealand White Rabbit ◽  
Femoral Diaphysis ◽  
New Zealand White Rabbit ◽  
Woven Bone ◽  
Freeze Dried ◽  
Demineralized Bone

Autogenous bone graft is gold standard in treating bone defects, but it might have difficulty in corporation and rejection reaction. This study is to compare the effectiveness among freeze-dried xenograft, freeze-dried allograft, hydroxyapatite xenograft, and demineralized bone matrix xenograft as bone graft to fill bone defect in femoral diaphysis of white rabbit. Thirty male New Zealand white rabbits were distributed into five groups. Bone defect was filled correspondingly with xenograft freeze-dried cortical bovine, allograft freeze-dried cortical New Zealand white rabbit, xenograft hydroxyapatite bovine, and xenograft demineralized bone matrix bovine. No graft was used in control group. VEGF, osteoblast, and woven bone were higher in allograft freeze-dried cortical New Zealand white rabbit (mean 5.6625 (p<0.05)) and xenograft demineralized bone matrix bovine (mean 5.2475 (p<0.05)) with calcification of woven bone was already seen in week 2 in the latter group. There was a decrease of woven bone (mean 4.685 (p<0.05)) fibrous tissue (mean 41.07 (p<0.05)) in xenograft demineralized bone matrix bovine. The Immunoglobulin-G was elevated in control and all study groups but not significantly (p=0.07855). Bone healing process in xenograft demineralized bone matrix bovine is more effective than in xenograft hydroxyapatite bovine, allograft freeze-dried New Zealand white rabbit, xenograft freeze-dried cortical bovine, and control.

scholarly journals Immunogenicity of Bone Graft Using Xenograft Freeze-Dried Cortical Bovine, Allograft Freeze-Dried Cortical New Zealand White Rabbit, Xenograft Hydroxyapatite Bovine, And Xenograft Demineralized Bone Matrix Bovine In Bone Defect Of Femoral Diaphysis White Rabbit Experimental Study In Vivo

2017 ◽  
Vol 3 (6) ◽  
pp. 344 ◽  
Author(s):  
Ferdiansyah Ferdiansyah ◽  
Dwikora Novembri Utomo ◽  
Heri Suroto
Keyword(s):  
Bone Graft ◽  
Bone Defect ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Zealand White Rabbit ◽  
Femoral Diaphysis ◽  
Autogenous Bone ◽  
Rejection Reaction ◽  
Freeze Dried ◽  
Demineralized Bone

Bone defect remains a big challenge for orthopedic surgeon. Bone grafting nowadays become the second common transplantation after blood transfusion. Autogenous bone graft is the gold standard in treatment of bone defect, but it’s source limitation and donor site morbidity makes some surgeon were looking for allograft or xenograft. There are some issues with allo- and xenograft about difficulty in corporation and rejection reaction. This study explores the immunogenicity of allograft and xenograft. Keyword :  freeze-dried xenograft, freeze-dried allograft, hydroxyapatite xenograft, demineralized bone matrix xenograft.

Effect of Allogenic Freeze-Dried Demineralized Bone Matrix on Guided Tissue Regeneration in Dogs

1998 ◽  
Vol 69 (8) ◽  
pp. 851-856 ◽  
Author(s):  
Nicholas Caplanis ◽  
Michael B. Lee ◽  
Grenith J. Zimmerman ◽  
Knut A. Selvig ◽  
Ulf M.E. Wikesjö
Keyword(s):  
Tissue Regeneration ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Guided Tissue Regeneration ◽  
Freeze Dried ◽  
Demineralized Bone

Early Healing of Freeze-Dried and Freeze-Dried Demineralized Bone Segments in Major Ulnar Defects in the Rabbit

1991 ◽  
Vol 5 (2) ◽  
pp. 213
Author(s):  
T. Moore ◽  
G. Sigholm ◽  
E. Gendler ◽  
G. J. Marshall ◽  
H. A. McKellop ◽  
...  
Keyword(s):  
Freeze Dried ◽  
Early Healing ◽  
Demineralized Bone

Periodontal Repair in Dogs: Effect of Allogenic Freeze-Dried Demineralized Bone Matrix Implants on Alveolar Bone and Cementum Regeneration

1998 ◽  
Vol 69 (1) ◽  
pp. 26-33 ◽  
Author(s):  
Chong-Kwan Kim ◽  
Kyoo-Sung Cho ◽  
Seong-Ho Choi ◽  
Annamarie Prewett ◽  
Ulf M.E. Wikesjö
Keyword(s):  
Alveolar Bone ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Freeze Dried ◽  
Demineralized Bone

scholarly journals Preparation and Characterization of Moldable Demineralized Bone Matrix/Calcium Sulfate Composite Bone Graft Materials

2021 ◽  
Vol 12 (4) ◽  
pp. 56
Author(s):  
I-Cheng Chen ◽  
Chen-Ying Su ◽  
Chun-Cheih Lai ◽  
Yi-Syue Tsou ◽  
Yudong Zheng ◽  
...  
Keyword(s):  
Bone Graft ◽  
Calcium Sulfate ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Washout Rate ◽  
Graft Material ◽  
Noncollagenous Proteins ◽  
Freeze Dried ◽  
Demineralized Bone ◽  
Composite Bone

Demineralized bone matrix (DBM) is a decalcified allo/xenograft retaining collagen and noncollagenous proteins, which has been extensively used because of its osteoconductive and osteoinductive properties. Calcium sulfate (CaSO4, CS) is a synthetic bone substitute used in bone healing with biocompatible, nontoxic, bioabsorbable, osteoconductive, and good mechanical characteristics. This study aims to prepare a DBM/CS composite bone graft material in a moldable putty form without compromising the peculiar properties of DBM and CS. For this purpose, firstly, porcine femur was defatted using chloroform/methanol and extracted by acid for demineralization, then freeze-dried and milled/sieved to obtain DBM powder. Secondly, the α-form and β-form of calcium sulfate hemihydrate (CaSO4 •0.5H2O, CSH) were produced by heating gypsum (CaSO4 •2H2O). The morphology and particle sizes of α- and β-CSH were obtained by SEM, and their chemical properties were confirmed by EDS, FTIR and XRD. Furthermore, the DBM-based graft was mixed with α- or β-CSH at a ratio of 9:1, and glycerol/4% HPMC was added as a carrier to produce a putty. DBM/CSH putty possesses a low washout rate, good mechanical strength and biocompatibility. In conclusion, we believe that the moldable DBM/CSH composite putty developed in this study could be a promising substitute for the currently available bone grafts, and might have practical application in the orthopedics field as a potential bone void filler.

Effect of allogeneic freeze-dried demineralized bone matrix on regeneration of alveolar bone and periodontal attachment in dogs

1998 ◽  
Vol 25 (10) ◽  
pp. 801-806 ◽  
Author(s):  
Nicholas Caplanis ◽  
Michael B. Lee ◽  
Grenith J. Zimmerman ◽  
Knut A. Selvig ◽  
Ulf M.E. Wikesjo
Keyword(s):  
Alveolar Bone ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Freeze Dried ◽  
Periodontal Attachment ◽  
Demineralized Bone
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