scholarly journals Immunogenicity of Bone Graft Using Xenograft Freeze-Dried Cortical Bovine, Allograft Freeze-Dried Cortical New Zealand White Rabbit, Xenograft Hydroxyapatite Bovine, And Xenograft Demineralized Bone Matrix Bovine In Bone Defect Of Femoral Diaphysis White Rabbit Experimental Study In Vivo

2017 ◽  
Vol 3 (6) ◽  
pp. 344 ◽  
Author(s):  
Ferdiansyah Ferdiansyah ◽  
Dwikora Novembri Utomo ◽  
Heri Suroto
Keyword(s):  
Bone Graft ◽  
Bone Defect ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Zealand White Rabbit ◽  
Femoral Diaphysis ◽  
Autogenous Bone ◽  
Rejection Reaction ◽  
Freeze Dried ◽  
Demineralized Bone

Bone defect remains a big challenge for orthopedic surgeon. Bone grafting nowadays become the second common transplantation after blood transfusion. Autogenous bone graft is the gold standard in treatment of bone defect, but it’s source limitation and donor site morbidity makes some surgeon were looking for allograft or xenograft. There are some issues with allo- and xenograft about difficulty in corporation and rejection reaction. This study explores the immunogenicity of allograft and xenograft. Keyword :  freeze-dried xenograft, freeze-dried allograft, hydroxyapatite xenograft, demineralized bone matrix xenograft.

scholarly journals Comparative Effectiveness of Bone Grafting Using Xenograft Freeze-Dried Cortical Bovine, Allograft Freeze-Dried Cortical New Zealand White Rabbit, Xenograft Hydroxyapatite Bovine, and Xenograft Demineralized Bone Matrix Bovine in Bone Defect of Femoral Diaphysis of White Rabbit: Experimental Study In Vivo

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Ferdiansyah Mahyudin ◽  
Dwikora Novembri Utomo ◽  
Heri Suroto ◽  
Tri Wahyu Martanto ◽  
Mouli Edward ◽  
...  
Keyword(s):  
New Zealand ◽  
Bone Defect ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Zealand White Rabbit ◽  
Femoral Diaphysis ◽  
New Zealand White Rabbit ◽  
Woven Bone ◽  
Freeze Dried ◽  
Demineralized Bone

Autogenous bone graft is gold standard in treating bone defects, but it might have difficulty in corporation and rejection reaction. This study is to compare the effectiveness among freeze-dried xenograft, freeze-dried allograft, hydroxyapatite xenograft, and demineralized bone matrix xenograft as bone graft to fill bone defect in femoral diaphysis of white rabbit. Thirty male New Zealand white rabbits were distributed into five groups. Bone defect was filled correspondingly with xenograft freeze-dried cortical bovine, allograft freeze-dried cortical New Zealand white rabbit, xenograft hydroxyapatite bovine, and xenograft demineralized bone matrix bovine. No graft was used in control group. VEGF, osteoblast, and woven bone were higher in allograft freeze-dried cortical New Zealand white rabbit (mean 5.6625 (p<0.05)) and xenograft demineralized bone matrix bovine (mean 5.2475 (p<0.05)) with calcification of woven bone was already seen in week 2 in the latter group. There was a decrease of woven bone (mean 4.685 (p<0.05)) fibrous tissue (mean 41.07 (p<0.05)) in xenograft demineralized bone matrix bovine. The Immunoglobulin-G was elevated in control and all study groups but not significantly (p=0.07855). Bone healing process in xenograft demineralized bone matrix bovine is more effective than in xenograft hydroxyapatite bovine, allograft freeze-dried New Zealand white rabbit, xenograft freeze-dried cortical bovine, and control.

scholarly journals Histopathological and Radiographical Evaluation of Caprine Demineralized Bone Matrix in a Critical Ulnar Defect in a Rabbit Model

2021 ◽  
Author(s):  
Olawale Alimi Alimi ◽  
Adamu Abdul Abuabakar ◽  
Abubakar Sadiq Yakubu ◽  
Sani Abdullahi Shehu ◽  
Salman Zubairu Abdulkadir
Keyword(s):  
Bone Defect ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Rabbit Model ◽  
Negative Control ◽  
Autogenous Bone ◽  
Level Of Evidence ◽  
Defect Sites ◽  
Significant Difference ◽  
Demineralized Bone

Abstract Background: Caprine species satisfy the conditions of an ideal donor animal when compared to bovine species that has been extensively studied and commercialized for bone xenograft. Histopathological and radiological evaluations of caprine demineralized bone matrix (CDBM) were therefore carried out for fracture healing properties for its possible use in bone grafting procedures. Materials and Methods: Twenty-four rabbits were used for this study and were divided randomly into three groups of eight (n=8) rabbits each. Critical bone defect was created on the ulnar diaphysis under xylazine-ketamine anaesthesia for autogenous bone graft (ABG) group, CDBM group and the last group was left unfilled as negative control (NC). Immediate post-grafting radiograph was taken and repeated on days 14, 28, 42 and 56 to monitor the evidence of radiographic healing. The animals were euthanized on day 56 and defect sites were harvested for histopathology. Results: There was a progressive evidence of radiographic healing and bone formation in all the groups with significance difference (P=0.0064). When compared with ABG, NC differ significantly (P<0.0001) whereas the CDBM did not differ significantly (P=0.6765). The histopathology sections of ABG and CDBM showed normal bone tissue while the NC section was predominated by fibrous connective tissue. There was therefore an overall significant difference (P=0.0001) in which CDBM did not differ from ABG (P=0.2946) while NC did (P=0.0005). Conclusion: The ABG and CDBM groups showed a similar healing effect in the critical bone defect. Therefore, CDBM could be used as an effective alternative to ABG in orthopaedics to circumvent the limitations and complications associated with it. Level of Evidence: Not applicable.

scholarly journals Preparation and Characterization of Moldable Demineralized Bone Matrix/Calcium Sulfate Composite Bone Graft Materials

2021 ◽  
Vol 12 (4) ◽  
pp. 56
Author(s):  
I-Cheng Chen ◽  
Chen-Ying Su ◽  
Chun-Cheih Lai ◽  
Yi-Syue Tsou ◽  
Yudong Zheng ◽  
...  
Keyword(s):  
Bone Graft ◽  
Calcium Sulfate ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Washout Rate ◽  
Graft Material ◽  
Noncollagenous Proteins ◽  
Freeze Dried ◽  
Demineralized Bone ◽  
Composite Bone

Demineralized bone matrix (DBM) is a decalcified allo/xenograft retaining collagen and noncollagenous proteins, which has been extensively used because of its osteoconductive and osteoinductive properties. Calcium sulfate (CaSO4, CS) is a synthetic bone substitute used in bone healing with biocompatible, nontoxic, bioabsorbable, osteoconductive, and good mechanical characteristics. This study aims to prepare a DBM/CS composite bone graft material in a moldable putty form without compromising the peculiar properties of DBM and CS. For this purpose, firstly, porcine femur was defatted using chloroform/methanol and extracted by acid for demineralization, then freeze-dried and milled/sieved to obtain DBM powder. Secondly, the α-form and β-form of calcium sulfate hemihydrate (CaSO4 •0.5H2O, CSH) were produced by heating gypsum (CaSO4 •2H2O). The morphology and particle sizes of α- and β-CSH were obtained by SEM, and their chemical properties were confirmed by EDS, FTIR and XRD. Furthermore, the DBM-based graft was mixed with α- or β-CSH at a ratio of 9:1, and glycerol/4% HPMC was added as a carrier to produce a putty. DBM/CSH putty possesses a low washout rate, good mechanical strength and biocompatibility. In conclusion, we believe that the moldable DBM/CSH composite putty developed in this study could be a promising substitute for the currently available bone grafts, and might have practical application in the orthopedics field as a potential bone void filler.

Mechanical advantage of defects healed with demineralized bone matrix over autogenous bone graft

2002 ◽  
Vol 2 (5) ◽  
pp. 101
Author(s):  
Celeste Abjornson ◽  
Marjolein Van Der Muelen ◽  
Elizabeth Myers ◽  
Jocelyn Cottrell ◽  
Emre Tomin ◽  
...  
Keyword(s):  
Bone Graft ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Autogenous Bone Graft ◽  
Autogenous Bone ◽  
Mechanical Advantage ◽  
Demineralized Bone

Comparative Assessment of Autogenous Cancellous Bone Graft and Bovine-Derived Demineralized Bone Matrix for Secondary Alveolar Bone Grafting in Patients With Unilateral Cleft Lip and Palate

2021 ◽  
pp. 105566562110251
Author(s):  
Vijay Kumar ◽  
Vidya Rattan ◽  
Sachin Rai ◽  
Satinder Pal Singh ◽  
Jai Kumar Mahajan
Keyword(s):  
Bone Graft ◽  
Iliac Crest ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Volumetric Analysis ◽  
Group I ◽  
Alveolar Bone Grafting ◽  
Group Ii ◽  
Demineralized Bone

Objective: Comparison between bovine-derived demineralized bone matrix (DMBM) and iliac crest graft over long term for secondary alveolar bone grafting (SABG) in patients with unilateral cleft lip and palate (UCLP) in terms of radiological and clinical outcomes. Design: Prospective, randomized, parallel groups, double-blind, controlled trial. Setting: Unit of Oral and Maxillofacial Surgery, Oral Health Science Centre, Postgraduate Institute of Medical Education & Research, Chandigarh. Participants: Twenty patients with UCLP. Interventions: Patients were allocated into group I (Iliac crest bone graft) and group II (DMBM) for SABG. Outcomes were assessed at 2 weeks, 6 months, and then after mean follow-up period of 63 months. Outcomes Measures: Volumetric analysis of the grafted bone in the alveolar cleft site was done through cone beam computed tomography using Cavalieri principle and modified assessment tool. Clinical assessment was performed in terms of pain, swelling, duration of hospital stay, cost of surgery, alar base symmetry, and donor site morbidity associated with iliac crest harvesting. Results: Volumetric analysis through Cavalieri principle revealed comparable bone uptake at follow-up of 6 months between group I (70%) and group II (69%). Modified assessment tool showed no significant difference between horizontal and vertical bone scores over short- and long-term follow-up. In group II, there was higher cost of surgery, but no donor site morbidity unlike group I. Conclusions: Demineralized bone matrix proved analogous to iliac crest bone graft as per volumetric analysis over shorter period. However, although statistically insignificant, net bone volume achieved was lower than the iliac crest graft at longer follow-up.

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