The effect of the freeze dried bone allograft and gel/putty type demineralized bone matrix on osseous regeneration in the rat calvarial defects

Cadaveric decellularized bone tissue is utilized as an allograft in many musculoskeletal surgical procedures. Typically, the allograft acts as a scaffold to guide tissue regeneration with superior biocompatibility relative to synthetic scaffolds. Traditionally these scaffolds are machined into the required dimensions and shapes. However, the geometrical simplicity and, in some cases, limited dimensions of the donated tissue restrict the use of allograft scaffolds. This could be overcome by additive manufacturing using granulated bone that is both decellularized and demineralized. In this study, the large area projection sintering (LAPS) method is evaluated as a fabrication method to build porous structures composed of granulated cortical bone bound by polycaprolactone (PCL). This additive manufacturing method utilizes visible light to selectively cure the deposited material layer-by-layer to create 3D geometry. First, the spreading behavior of the composite mixtures is evaluated and the conditions to attain improved powder bed density to fabricate the test specimens are determined. The tensile strength of the LAPS fabricated samples in both dry and hydrated states are determined and compared to the demineralized cancellous bone allograft and the heat treated demineralized-bone/PCL mixture in mold. The results indicated that the projection sintered composites of 45–55 wt %. Demineralized bone matrix (DBM) particulates produced strength comparable to processed and demineralized cancellous bone.

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First Human Leucocyte Antigen (HLA) Response and Safety Evaluation of Fibrous Demineralized Bone Matrix in a Critical Size Femoral Defect Model of the Sprague-Dawley Rat

Materials ◽

10.3390/ma13143120 ◽

2020 ◽

Vol 13 (14) ◽

pp. 3120

Author(s):

Nicolas Söhling ◽

Maximilian Leiblein ◽

Alexander Schaible ◽

Maren Janko ◽

Joachim Schwäble ◽

...

Keyword(s):

Bone Healing ◽

Critical Size ◽

Demineralized Bone Matrix ◽

High Surface ◽

Bone Matrix ◽

Volume Ratio ◽

Bone Allograft ◽

Control Group ◽

Sprague Dawley ◽

Demineralized Bone

Treatment of large bone defects is one of the great challenges in contemporary orthopedic and traumatic surgery. Grafts are necessary to support bone healing. A well-established allograft is demineralized bone matrix (DBM) prepared from donated human bone tissue. In this study, a fibrous demineralized bone matrix (f-DBM) with a high surface-to-volume ratio has been analyzed for toxicity and immunogenicity. f-DBM was transplanted to a 5-mm, plate-stabilized, femoral critical-size-bone-defect in Sprague-Dawley (SD)-rats. Healthy animals were used as controls. After two months histology, hematological analyses, immunogenicity as well as serum biochemistry were performed. Evaluation of free radical release and hematological and biochemical analyses showed no significant differences between the control group and recipients of f-DBM. Histologically, there was no evidence of damage to liver and kidney and good bone healing was observed in the f-DBM group. Reactivity against human HLA class I and class II antigens was detected with mostly low fluorescence values both in the serum of untreated and treated animals, reflecting rather a background reaction. Taken together, these results provide evidence for no systemic toxicity and the first proof of no basic immunogenic reaction to bone allograft and no sensitization of the recipient.

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Characterization of Matrix-Induced Osteogenesis in Rat Calvarial Bone Defects: I. Differences in the Cellular Response to Demineralized Bone Matrix Implanted in Calvarial Defects and in Subcutaneous Sites

Calcified Tissue International ◽

10.1007/s002239900676 ◽

1999 ◽

Vol 65 (2) ◽

pp. 156-165 ◽

Cited By ~ 49

Author(s):

J. Wang ◽

M. J. Glimcher

Keyword(s):

Cellular Response ◽

Demineralized Bone Matrix ◽

Bone Matrix ◽

Bone Defects ◽

Calvarial Bone ◽

Calvarial Defects ◽

Demineralized Bone

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Comparative Effectiveness of Bone Grafting Using Xenograft Freeze-Dried Cortical Bovine, Allograft Freeze-Dried Cortical New Zealand White Rabbit, Xenograft Hydroxyapatite Bovine, and Xenograft Demineralized Bone Matrix Bovine in Bone Defect of Femoral Diaphysis of White Rabbit: Experimental Study In Vivo

International Journal of Biomaterials ◽

10.1155/2017/7571523 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 4

Author(s):

Ferdiansyah Mahyudin ◽

Dwikora Novembri Utomo ◽

Heri Suroto ◽

Tri Wahyu Martanto ◽

Mouli Edward ◽

...

Keyword(s):

New Zealand ◽

Bone Defect ◽

Demineralized Bone Matrix ◽

Bone Matrix ◽

Zealand White Rabbit ◽

Femoral Diaphysis ◽

New Zealand White Rabbit ◽

Woven Bone ◽

Freeze Dried ◽

Demineralized Bone

Autogenous bone graft is gold standard in treating bone defects, but it might have difficulty in corporation and rejection reaction. This study is to compare the effectiveness among freeze-dried xenograft, freeze-dried allograft, hydroxyapatite xenograft, and demineralized bone matrix xenograft as bone graft to fill bone defect in femoral diaphysis of white rabbit. Thirty male New Zealand white rabbits were distributed into five groups. Bone defect was filled correspondingly with xenograft freeze-dried cortical bovine, allograft freeze-dried cortical New Zealand white rabbit, xenograft hydroxyapatite bovine, and xenograft demineralized bone matrix bovine. No graft was used in control group. VEGF, osteoblast, and woven bone were higher in allograft freeze-dried cortical New Zealand white rabbit (mean 5.6625 (p<0.05)) and xenograft demineralized bone matrix bovine (mean 5.2475 (p<0.05)) with calcification of woven bone was already seen in week 2 in the latter group. There was a decrease of woven bone (mean 4.685 (p<0.05)) fibrous tissue (mean 41.07 (p<0.05)) in xenograft demineralized bone matrix bovine. The Immunoglobulin-G was elevated in control and all study groups but not significantly (p=0.07855). Bone healing process in xenograft demineralized bone matrix bovine is more effective than in xenograft hydroxyapatite bovine, allograft freeze-dried New Zealand white rabbit, xenograft freeze-dried cortical bovine, and control.

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Reconstruction of the Bone-Bone Marrow Organ by Osteogenin, a Bone Morphogenetic Protein, and Demineralized Bone Matrix in Calvarial Defects of Adult Primates

Plastic & Reconstructive Surgery ◽

10.1097/00006534-199301000-00005 ◽

1993 ◽

Vol 91 (1) ◽

pp. 27-36 ◽

Cited By ~ 51

Author(s):

Ugo Ripamonti ◽

Shu-Shan Ma ◽

Noreen S. Cunningham ◽

Laura Yeates ◽

A. Hari Reddi

Keyword(s):

Bone Marrow ◽

Bone Morphogenetic Protein ◽

Demineralized Bone Matrix ◽

Bone Matrix ◽

Morphogenetic Protein ◽

Calvarial Defects ◽

Demineralized Bone

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Immunogenicity of Bone Graft Using Xenograft Freeze-Dried Cortical Bovine, Allograft Freeze-Dried Cortical New Zealand White Rabbit, Xenograft Hydroxyapatite Bovine, And Xenograft Demineralized Bone Matrix Bovine In Bone Defect Of Femoral Diaphysis White Rabbit Experimental Study In Vivo

KnE Life Sciences ◽

10.18502/kls.v3i6.1143 ◽

2017 ◽

Vol 3 (6) ◽

pp. 344 ◽

Cited By ~ 1

Author(s):

Ferdiansyah Ferdiansyah ◽

Dwikora Novembri Utomo ◽

Heri Suroto

Keyword(s):

Bone Graft ◽

Bone Defect ◽

Demineralized Bone Matrix ◽

Bone Matrix ◽

Zealand White Rabbit ◽

Femoral Diaphysis ◽

Autogenous Bone ◽

Rejection Reaction ◽

Freeze Dried ◽

Demineralized Bone

Bone defect remains a big challenge for orthopedic surgeon. Bone grafting nowadays become the second common transplantation after blood transfusion. Autogenous bone graft is the gold standard in treatment of bone defect, but it’s source limitation and donor site morbidity makes some surgeon were looking for allograft or xenograft. There are some issues with allo- and xenograft about difficulty in corporation and rejection reaction. This study explores the immunogenicity of allograft and xenograft. Keyword : freeze-dried xenograft, freeze-dried allograft, hydroxyapatite xenograft, demineralized bone matrix xenograft.

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