In situ synthesis of magnesium-substituted biphasic calcium phosphate and in vitro biodegradation

Magnesium ion substituted biphasic calcium phosphate (Mg-BCP) bioceramic microscaffolds with spherical and porous morphology were successfully prepared usingin situcoprecipitation and rotary spray drying atomization process for application of tissue engineering combined with human adipose tissue-derived mesenchymal stem cells (hAT-MSCs). After 4 weeks of immersion in Hanks’ balanced salt solution (HBSS), Mg-BCP micro-scaffolds showed the enhanced biodegradation and bioactivity due to the substituted Mg2+ion present in the BCP structure. In this study, it was observed that hAT-MSCs have clearly attached on the surface of Mg-BCP micro-scaffolds. In addition, Mg-BCP micro-scaffolds exhibited the improved biocompatibility and osteoconductivity viain vitroandin vivobiological tests with hAT-MSCs. Therefore, these bioceramic micro-scaffolds had potential to be used as hAT-MSCs microcarriers for biomedical applications.

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IN-VITRO BEHAVIOUR OF BIPHASIC CALCIUM PHOSPHATE WITH DIFFERENT RATIO OF SILICA CONTENT IN SIMULATED BODY FLUID

Journal of industrial technology ◽

10.21908/jit.2015.8 ◽

2015 ◽

Vol 23 (1) ◽

pp. 1-14

Author(s):

Sudirman Sahid ◽

◽

Nor Shahida Kader Bashah ◽

Salina Sabudin ◽

◽

...

Keyword(s):

Calcium Phosphate ◽

Simulated Body Fluid ◽

Body Fluid ◽

Biphasic Calcium Phosphate ◽

Silica Content

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Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials

Microscopy Research and Technique ◽

10.1002/jemt.20374 ◽

2006 ◽

Vol 69 (12) ◽

pp. 976-982 ◽

Cited By ~ 29

Author(s):

Nenad Ignjatović ◽

Petar Ninkov ◽

Vesna Kojić ◽

Miloš Bokurov ◽

Vladimir Srdić ◽

...

Keyword(s):

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

In Vitro Test ◽

Vitro Test

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Strontium and fluorine co-doped biphasic calcium phosphate: characterization and in vitro cytocompatibility analysis

Biomedical Physics & Engineering Express ◽

10.1088/2057-1976/aa768c ◽

2017 ◽

Vol 3 (4) ◽

pp. 045004

Author(s):

Elmira Pourreza ◽

Ammar Z Alshemary ◽

Bengi Yilmaz ◽

Reza Moonesi Rad ◽

Aysen Tezcaner ◽

...

Keyword(s):

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

Co Doped

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Study of Bio-Mineralization of BCP with Compensation of Calcium and Phosphate Ions

Materials Science Forum ◽

10.4028/www.scientific.net/msf.610-613.1391 ◽

2009 ◽

Vol 610-613 ◽

pp. 1391-1394

Author(s):

Hua De Zheng ◽

Ying Jun Wang ◽

Qiang Ma ◽

Cheng Yun Ning ◽

Xiao Feng Chen

Keyword(s):

Free Energy ◽

Calcium Phosphate ◽

Calcium Ions ◽

Biphasic Calcium Phosphate ◽

Porous Ceramic ◽

Apatite Layer ◽

Apatite Formation ◽

Phosphate Ions

In the present study, an Intelligent Multi-parameter Simulated Evaluation in vitro （IMSE system） was used to study the deposition properties of apatite formation on the surface of biphasic calcium phosphate porous ceramic (BCP) from static and dynamic r-SBF. Results showed that apatite formed on the surface of BCP from static and dynamic r-SBF differed between each other. In static r-SBF, ions were transferred by diffusion, which could not compensate the consuming of calcium ions, and mist apatite layer was formed on the surface of samples. But in the dynamic r-SBF, simulated fluid was adjusted precisely and flowed forcedly, the concentrations of ions were homogeneous; with the compensation of ions, calcium and phosphate were supersaturated, and the free energy of apatite formation was negative, bone-like apatite sheets were formed on the surface of samples.

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