Study of Bio-Mineralization of BCP with Compensation of Calcium and Phosphate Ions

In the present study, an Intelligent Multi-parameter Simulated Evaluation in vitro （IMSE system） was used to study the deposition properties of apatite formation on the surface of biphasic calcium phosphate porous ceramic (BCP) from static and dynamic r-SBF. Results showed that apatite formed on the surface of BCP from static and dynamic r-SBF differed between each other. In static r-SBF, ions were transferred by diffusion, which could not compensate the consuming of calcium ions, and mist apatite layer was formed on the surface of samples. But in the dynamic r-SBF, simulated fluid was adjusted precisely and flowed forcedly, the concentrations of ions were homogeneous; with the compensation of ions, calcium and phosphate were supersaturated, and the free energy of apatite formation was negative, bone-like apatite sheets were formed on the surface of samples.

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In Vitro Evaluation of RF Magnetron-Sputtered Calcium Phosphate Films on Titanium

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.352.305 ◽

2007 ◽

Vol 352 ◽

pp. 305-309

Author(s):

Kyosuke Ueda ◽

Takayuki Narushima ◽

Tomoyuki Katsube ◽

Hiroshi Kawamura ◽

Takashi Goto

Keyword(s):

Calcium Phosphate ◽

Calcium Ions ◽

Rf Magnetron Sputtering ◽

Calcium Phosphate Coating ◽

Apatite Formation ◽

Coating Films ◽

Before And After ◽

Phosphate Buffered Saline ◽

Titanium Substrates

Calcium phosphate coating films were fabricated on mirror-polished or blast-treated titanium substrates using radio-frequency (RF) magnetron sputtering and they were evaluated in vitro. Immersion tests for the films were conducted using phosphate-buffered saline (PBS(-)), and apatite formation and the elution of calcium ions from the films were investigated. The bonding strengths between the calcium phosphate films and titanium substrates before and after the immersion tests were evaluated. After the immersion tests, a decrease in the bonding strength was observed for the coating films on the mirror-polished titanium substrates, while that for the blast-treated titanium substrates was almost the same as that before the immersion tests.

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Synthesis and Characterization of Nanoporous Biphasic Calcium Phosphate

Journal of Physics Conference Series ◽

10.1088/1742-6596/2071/1/012009 ◽

2021 ◽

Vol 2071 (1) ◽

pp. 012009

Author(s):

Nur Farahiyah Mohammad ◽

Koo Li Mei ◽

Mohd Riza Mohd Roslan ◽

Siti Shuhadah Md Saleh ◽

Farah Diana Mohd Daud

Keyword(s):

Calcium Phosphate ◽

Pore Size ◽

Biphasic Calcium Phosphate ◽

Morphological Structure ◽

Xrd Analysis ◽

Precipitation Method ◽

Chemical Compositions ◽

Apatite Formation ◽

Distilled Water

Abstract For the past few years, many researchers are focusing on biomaterials fabrication in porous form. The research on porous calcium phosphate has been investigated due to its excellent biocompatibility and better osseointegration. This research paper presented nanoporous biphasic calcium phosphate (BCP) synthesised using chemical precipitation method. Triblock co-polymer F127 was used as pore directing agent. The chemical compositions of pure BCP samples were examined using X-ray diffraction (XRD) analysis which shows common peak of BCP. The pore size distribution (PSD) on the other hand shows that the pore size of the samples mainly distributed at 52.8 nm, 49.6 nm and 32 nm. BCP pellets were soaked in phosphates buffered saline (PBS) and distilled water (DW) for 15 days. The pH of the soaking medium decreases throughout the soaking period due to degradation of BCP pellets, which release hydrogen ions into the PBS and distilled water. BCP degrades faster in distilled water than in PBS. After soaking for 15 days, materials were examined with a scanning electron microscope (SEM) to assess the morphological structure before and after in vitro degradation. Apatite formation was discovered on the surface of the BCP pellet that had been immersed in the PBS solution.

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Effect of Microstructure on Osteoinductivity of Biomaterials

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.284-286.289 ◽

2005 ◽

Vol 284-286 ◽

pp. 289-292 ◽

Cited By ~ 4

Author(s):

Ji Yong Chen ◽

You Rong Duan ◽

Xing Dong Zhang

Keyword(s):

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

Animal Experiments ◽

Apatite Layer ◽

Apatite Formation ◽

Calcium Phosphate Ceramics ◽

Group A ◽

Group B ◽

Group D ◽

Porous Biphasic Calcium Phosphate

Two sets of porous biphasic calcium phosphate ceramics (BCP) were prepared for dynamic SBF experiment: porous BCP with micropores on the walls of macropores( set A) and porous BCP with dense walls of macropores (set B). Apatite layer could only formed on the macropore walls with micropores. Four groups of specimens were prepared for animal experiments. Group A was porous BCP ceramics with micropores on the walls of macropores; group B was porous BCP with dense walls of macropores; group C was porous BCP ceramics with apatite layers formed by static SBF[2]on their surfaces; group D was porous BCP ceramics with apatite layers formed by dynamic SBF on their walls of macropores. The result of dynamic SBF animal experiments showed that microstructure of BCP played an important role in the bone-like apatite formation and osteoinductiion in biomaterials. Apatite formation may be the prerequisite of osteoinductive formation of new bone.

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THE EFFECT OF CASEIN PHOSPHOPEPTIDE-AMORPHOUS CALCIUM PHOSPHATE-PROPOLIS CHEWING GUM ON CALCIUM AND PHOSPHATE ION LEVELS IN CARIES-FREE SUBJECT’S SALIVA AND STREPTOCOCCUS MUTANS BIOFILM FORMATION

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10s5.23125 ◽

2017 ◽

Vol 10 (17) ◽

pp. 174

Author(s):

Sri Angky Soekanto ◽

Gadiacanaparimita Ghrena Duhita ◽

Endang Winiati Bachtiar ◽

Muhamad Sahlan

Keyword(s):

Calcium Phosphate ◽

Streptococcus Mutans ◽

Calcium Ions ◽

Amorphous Calcium Phosphate ◽

Chewing Gum ◽

Phosphate Ion ◽

Phosphate Ions ◽

Casein Phosphopeptide ◽

Mass Formation

Objective: The aim of this study is to analyze the amount of calcium and phosphate ion released by casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), which supports the remineralization and the effectiveness of propolis wax in suppressing the mass formation of Streptococcus mutans biofilm on caries-free subjects and to observe if these two active components are effective when combined into one substance and placed inside sugar-free chewing gum.Methods: Twenty-five samples of caries-free saliva before and after the mastication simulation (five concentrations of chewing gum) in vitro were observed to check for release of calcium and phosphate ions along with a biofilm assay. The release of calcium ions was detected using AAS, the release of phosphate ions was detected using spectrophotometry ultraviolet-visible, and the biofilm assay was detected using a crystal violet 96-well plate ELISA and was evaluated with an ELISA reader.Result: Chewing gum with a concentration of 0% prop + 5% CPP-ACP showed the highest release level of calcium ions (p<0.05) and phosphate ions (p>0.05) and is significant in suppressing the mass formation of the S. mutans biofilm (p<0.05).Conclusions: Mastication simulation of CPP-ACP-Propolis chewing gum can increase calcium and phosphate ion levels in caries-free saliva and decrease S. mutans biofilm mass formation; this can support remineralization and becomes a dental caries prevention alternative.

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IN-VITRO BEHAVIOUR OF BIPHASIC CALCIUM PHOSPHATE WITH DIFFERENT RATIO OF SILICA CONTENT IN SIMULATED BODY FLUID

Journal of industrial technology ◽

10.21908/jit.2015.8 ◽

2015 ◽

Vol 23 (1) ◽

pp. 1-14

Author(s):

Sudirman Sahid ◽

◽

Nor Shahida Kader Bashah ◽

Salina Sabudin ◽

◽

...

Keyword(s):

Calcium Phosphate ◽

Simulated Body Fluid ◽

Body Fluid ◽

Biphasic Calcium Phosphate ◽

Silica Content

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Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials

Microscopy Research and Technique ◽

10.1002/jemt.20374 ◽

2006 ◽

Vol 69 (12) ◽

pp. 976-982 ◽

Cited By ~ 29

Author(s):

Nenad Ignjatović ◽

Petar Ninkov ◽

Vesna Kojić ◽

Miloš Bokurov ◽

Vladimir Srdić ◽

...

Keyword(s):

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

In Vitro Test ◽

Vitro Test

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Strontium and fluorine co-doped biphasic calcium phosphate: characterization and in vitro cytocompatibility analysis

Biomedical Physics & Engineering Express ◽

10.1088/2057-1976/aa768c ◽

2017 ◽

Vol 3 (4) ◽

pp. 045004

Author(s):

Elmira Pourreza ◽

Ammar Z Alshemary ◽

Bengi Yilmaz ◽

Reza Moonesi Rad ◽

Aysen Tezcaner ◽

...

Keyword(s):

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

Co Doped

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The pH-Dependent Properties of the Biphasic Calcium Phosphate for Bone Cements

Journal of Biomimetics Biomaterials and Biomedical Engineering ◽

10.4028/www.scientific.net/jbbbe.21.3 ◽

2014 ◽

Vol 21 ◽

pp. 3-16 ◽

Cited By ~ 5

Author(s):

Nuan La Ong Srakaew ◽

Sirirat Tubsungnoen Rattanachan

Keyword(s):

Compressive Strength ◽

Liquid Phase ◽

Calcium Phosphate ◽

Phase Analysis ◽

Calcium Phosphate Cement ◽

Biphasic Calcium Phosphate ◽

Setting Time ◽

Bone Cements ◽

Apatite Formation ◽

Apatite Cement

Self-setting calcium phosphate cement (CPC) has been used in bone repair and substitution due to their excellent biocompatibility, bioactive as well as simplicity of preparation and use. The inherent brittleness and slow degradation are the major disadvantages for the use of calcium phosphate cements. To improve the degradation for the traditional CPC, the apatite cement formula incorporated with β-tricalcium phosphate (β-TCP) with varying concentration were studied and the effect of the pH value of liquid phase on the properties of this new calcium phosphate cement formula was evaluated. The apatite cements containing β-TCP for 10 and 40 wt.% were mixed into the aqueous solution with different pH values and then aging in absolute humidity at 37°C for 7 days. The setting time and phase analysis of the biphasic calcium phosphate were determined as compared to the apatite cement. For proper medical application, the compressive strength, the phase analysis and the degradation of the CPC samples at pH 7.0 and 7.4 were evaluated after soaking in the simulated body fluid (SBF) at 37°C for 7 days. The results indicated that the properties of the samples such as the setting time, the compressive strength related to the phase analysis of the set cements. The high degradation of the CPC was found in the cement with increasing β-TCP addition due to the phase after setting. Apatite formation with oriented plate-like morphology was also found to be denser on the surface of the biphasic bone cements after soaking in SBF for 7 days. The obtained results indicated that the cement containing β-TCP mixed with the liquid phase at pH 7.4 could be considered as a highly biodegradable and bioactive bone cement, as compared to the traditional CPC.

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