In situ magnesium calcium phosphate cements formation: From one pot powders precursors synthesis to in vitro investigations

Calcium phosphate cements (CPCs) can be a suitable scaffold material for bone tissue engineering because of their osteoconductivity and perfect fit with the surrounding tissue when injected in situ. However, the main disadvantage of hydroxyapatite (HA) forming CPC is its slow degradation rate, which hinders complete bone regeneration. A new approach is to use hydraulic apatite cement with mainly α/β-tricalciumphosphate (TCP) instead of α-TCP. After hydrolysis the α/β-TCP transforms in a partially non-absorbable HA and a completely resorbable β-TCP phase. Therefore, α-TCP material was thermally treated at several temperatures and times resulting in different α/β-TCP ratios. In this experiment, we developed and evaluated injectable biphasic calcium phosphate cements (BCPC) in vitro. Biphasic α/β-TCP powder was produced by heating α-TCP ranging from 1000-11250°C. Setting time and compressive strength of the CPCs were analyzed after soaking in PBS for 6 weeks. Results demonstrated that the phase composition can be controlled by the sintering temperature. Heat treatment of α-TCP, resulted in 100%, 75% and 25% of α-to β-TCP transformation, respectively. Incorporation of these sintered BCP powder into the cement formulation increased the setting time of the CPC paste. Compressive strength decreased with increasing β-TCP content. In this study, biphasic CPCs were produced and characterized in vitro. This injectable biphasic CPC presented comparable properties to an apatitic CPC.

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Bactericidal and Bioresorbable Calcium Phosphate Cements Fabricated by Silver-Containing Tricalcium Phosphate Microspheres

International Journal of Molecular Sciences ◽

10.3390/ijms21113745 ◽

2020 ◽

Vol 21 (11) ◽

pp. 3745

Author(s):

Michiyo Honda ◽

Yusuke Kawanobe ◽

Kohei Nagata ◽

Ken Ishii ◽

Morio Matsumoto ◽

...

Keyword(s):

Calcium Phosphate ◽

Tricalcium Phosphate ◽

Bacterial Adhesion ◽

Inflammatory Responses ◽

Spray Pyrolysis Technique ◽

Biological Properties ◽

Histological Evaluation ◽

Bone Apposition ◽

Calcium Phosphate Cements

Bacterial adhesion to the calcium phosphate surface is a serious problem in surgery. To prevent bacterial infection, the development of calcium-phosphate cements (CPCs) with bactericidal properties is indispensable. The aim of this study was to fabricate antibacterial CPCs and evaluate their biological properties. Silver-containing tricalcium phosphate (Ag-TCP) microspheres consisting of α/β-TCP phases were synthesized by an ultrasonic spray-pyrolysis technique. The powders prepared were mixed with the setting liquid to fabricate the CPCs. The resulting cements consisting of β-TCP and hydroxyapatite had a porous structure and wash-out resistance. Additionally, silver and calcium ions could be released into the culture medium from Ag-TCP cements for a long time accompanied by the dissolution of TCP. These data showed the bioresorbability of the Ag-TCP cement. In vitro antibacterial evaluation demonstrated that both released and immobilized silver suppressed the growth of bacteria and prevented bacterial adhesion to the surface of CPCs. Furthermore, histological evaluation by implantation of Ag-TCP cements into rabbit tibiae exhibited abundant bone apposition on the cement without inflammatory responses. These results showed that Ag-TCP cement has a good antibacterial property and good biocompatibility. The present Ag-TCP cements are promising for bone tissue engineering and may be used as antibacterial biomaterials.

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The releasing behavior and in vitro osteoinductive evaluations of dexamethasone-loaded porous calcium phosphate cements

Ceramics International ◽

10.1016/j.ceramint.2013.06.107 ◽

2014 ◽

Vol 40 (1) ◽

pp. 1081-1091 ◽

Cited By ~ 12

Author(s):

Azin Forouzandeh ◽

Saeed Hesaraki ◽

Ali Zamanian

Keyword(s):

Calcium Phosphate ◽

Calcium Phosphate Cements

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Concise Synthesis of Tryptanthrin Spiro Analogues with In Vitro Antitumor Activity Based on One-Pot, Three-Component 1,3-Dipolar Cycloaddition of Azomethine Ylides to Сyclopropenes

Synthesis ◽

10.1055/s-0037-1611059 ◽

2018 ◽

Vol 51 (03) ◽

pp. 713-729 ◽

Cited By ~ 6

Author(s):

Vitali Boitsov ◽

Alexander Stepakov ◽

Alexander Filatov ◽

Nickolay Knyazev ◽

Stanislav Shmakov ◽

...

Keyword(s):

Antitumor Activity ◽

Azomethine Ylides ◽

Azomethine Ylide ◽

Dipolar Cycloaddition ◽

One Pot ◽

Economic Method ◽

Reaction Proceeds ◽

High Yields

A simple, efficient and atom-economic method has been developed for the synthesis of complex alkaloid-like compounds with spiro-fused indolo[2,1-b]quinazoline and cyclopropa[a]pyrrolizine or 3-azabicyclo[3.1.0]hexane moieties. We have found that one-pot, three-component 1,3-dipolar cycloaddition reactions allow the desired products to be obtained from various cyclopropene derivatives with tryptanthrin-derived azomethine ylides generated in situ, in good to high yields and excellent diastereoselectivity. The possibility of ylide generation was exemplified by using α-amino acids (l-proline, l-4-thiazolidincarboxylic acid) and simplest peptides (dipeptide Gly-Gly, tripeptide Gly-Gly-Gly). Quantum chemical investigations indicate that the reaction proceeds through the S-shaped azomethine ylide, the interaction of which with cyclopropenes proceeds via a less sterically hindered endo-transition state. The antitumor activity of some of spiro-tryptanthrin derivatives against erythroleukemia (K562), cervical carcinoma (HeLa) and colon carcinoma (CT26) cell lines was evaluated in vitro by MTS-assay.

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Role of macropore size in the mechanical properties and in vitro degradation of porous calcium phosphate cements

Materials Letters ◽

10.1016/j.matlet.2010.06.008 ◽

2010 ◽

Vol 64 (18) ◽

pp. 2028-2031 ◽

Cited By ~ 13

Author(s):

Bai Feng ◽

Meng Guolin ◽

Yuan Yuan ◽

Liu Changshen ◽

Wang Zhen ◽

...

Keyword(s):

Mechanical Properties ◽

Calcium Phosphate ◽

In Vitro Degradation ◽

Calcium Phosphate Cements

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Microwave assisted-in situ synthesis of porous titanium/calcium phosphate composites and their in vitro apatite-forming capability

Composites Part B Engineering ◽

10.1016/j.compositesb.2015.08.046 ◽

2015 ◽

Vol 83 ◽

pp. 50-57 ◽

Cited By ~ 20

Author(s):

Man-Tik Choy ◽

Chak-Yin Tang ◽

Ling Chen ◽

Wing-Cheung Law ◽

Chi-Pong Tsui ◽

...

Keyword(s):

Calcium Phosphate ◽

Porous Titanium ◽

In Situ Synthesis ◽

Microwave Assisted

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In vitro bioactivity and biocompatibility of calcium phosphate cements using Hydroxy-propyl-methyl-Cellulose (HPMC)

Applied Surface Science ◽

10.1016/j.apsusc.2010.08.091 ◽

2010 ◽

Vol 257 (5) ◽

pp. 1533-1539 ◽

Cited By ~ 15

Author(s):

M. Anirban Jyoti ◽

Van Viet Thai ◽

Young Ki Min ◽

Byong-Taek Lee ◽

Ho-Yeon Song

Keyword(s):

Calcium Phosphate ◽

Methyl Cellulose ◽

In Vitro Bioactivity ◽

Calcium Phosphate Cements

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In situ synthesis of magnesium-substituted biphasic calcium phosphate and in vitro biodegradation

Materials Research Bulletin ◽

10.1016/j.materresbull.2012.05.011 ◽

2012 ◽

Vol 47 (9) ◽

pp. 2506-2512 ◽

Cited By ~ 29

Author(s):

Tae-Wan Kim ◽

Hyeong-Shin Lee ◽

Dong-Hyun Kim ◽

Hyeong-Ho Jin ◽

Kyu-Hong Hwang ◽

...

Keyword(s):

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

In Situ Synthesis

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Regenerating Craniofacial Dental Defects With Calcium Phosphate Cement Scaffolds: Current Status and Innovative Scope Review

Frontiers in Dental Medicine ◽

10.3389/fdmed.2021.743065 ◽

2021 ◽

Vol 2 ◽

Author(s):

Rashed A. Alsahafi ◽

Heba Ahmed Mitwalli ◽

Abdulrahman A. Balhaddad ◽

Michael D. Weir ◽

Hockin H. K. Xu ◽

...

Keyword(s):

Tissue Engineering ◽

Calcium Phosphate ◽

Bone Regeneration ◽

Calcium Phosphate Cement ◽

Current Status ◽

Craniofacial Bone ◽

Calcium Phosphate Cements ◽

And Performance

The management and treatment of dental and craniofacial injuries have continued to evolve throughout the last several decades. Limitations with autograft, allograft, and synthetics created the need for more advanced approaches in tissue engineering. Calcium phosphate cements (CPC) are frequently used to repair bone defects. Since their discovery in the 1980s, extensive research has been conducted to improve their properties, and emerging evidence supports their increased application in bone tissue engineering. This review focuses on the up-to-date performance of calcium phosphate cement (CPC) scaffolds and upcoming promising dental and craniofacial bone regeneration strategies. First, we summarized the barriers encountered in CPC scaffold development. Second, we compiled the most up to date in vitro and in vivo literature. Then, we conducted a systematic search of scientific articles in MEDLINE and EMBASE to screen the related studies. Lastly, we revealed the current developments to effectively design CPC scaffolds and track the enhanced viability and therapeutic efficacy to overcome the current limitations and upcoming perspectives. Finally, we presented a timely and opportune review article focusing on the significant potential of CPC scaffolds for dental and craniofacial bone regeneration, which will be discussed thoroughly. CPC offers multiple capabilities that may be considered toward the oral defects, expecting a future outlook in nanotechnology design and performance.

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