Layered yttrium hydroxide composite as supersensitive fluorescent sensor on Fe(III) ions

Single-Molecule Imaging of Active Mitochondrial Nitroreductases using a Photo-Crosslinking Fluorescent Sensor

10.26434/chemrxiv.7992980.v1 ◽

2019 ◽

Author(s):

Zacharias Thiel ◽

Pablo Rivera-Fuentes

Keyword(s):

Subcellular Localization ◽

Fluorescent Probe ◽

Single Molecule ◽

Mammalian Cells ◽

Fluorescent Sensor ◽

Biological Functions ◽

Localization Microscopy ◽

Drug Activation ◽

Nitroreductase Activity ◽

Single Molecule Localization Microscopy

Many biomacromolecules are known to cluster in microdomains with specific subcellular localization. In the case of enzymes, this clustering greatly defines their biological functions. Nitroreductases are enzymes capable of reducing nitro groups to amines and play a role in detoxification and pro-drug activation. Although nitroreductase activity has been detected in mammalian cells, the subcellular localization of this activity remains incompletely characterized. Here, we report a fluorescent probe that enables super-resolved imaging of pools of nitroreductase activity within mitochondria. This probe is activated sequentially by nitroreductases and light to give a photo-crosslinked adduct of active enzymes. In combination with a general photoactivatable marker of mitochondria, we performed two-color, threedimensional, single-molecule localization microscopy. These experiments allowed us to image the sub-mitochondrial organization of microdomains of nitroreductase activity.<br>

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Single-Molecule Imaging of Active Mitochondrial Nitroreductases using a Photo-Crosslinking Fluorescent Sensor

10.26434/chemrxiv.7992980 ◽

2019 ◽

Author(s):

Zacharias Thiel ◽

Pablo Rivera-Fuentes

Keyword(s):

Subcellular Localization ◽

Fluorescent Probe ◽

Single Molecule ◽

Mammalian Cells ◽

Fluorescent Sensor ◽

Biological Functions ◽

Localization Microscopy ◽

Drug Activation ◽

Nitroreductase Activity ◽

Single Molecule Localization Microscopy

Many biomacromolecules are known to cluster in microdomains with specific subcellular localization. In the case of enzymes, this clustering greatly defines their biological functions. Nitroreductases are enzymes capable of reducing nitro groups to amines and play a role in detoxification and pro-drug activation. Although nitroreductase activity has been detected in mammalian cells, the subcellular localization of this activity remains incompletely characterized. Here, we report a fluorescent probe that enables super-resolved imaging of pools of nitroreductase activity within mitochondria. This probe is activated sequentially by nitroreductases and light to give a photo-crosslinked adduct of active enzymes. In combination with a general photoactivatable marker of mitochondria, we performed two-color, threedimensional, single-molecule localization microscopy. These experiments allowed us to image the sub-mitochondrial organization of microdomains of nitroreductase activity.<br>

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Faculty Opinions recommendation of Near-infrared fluorescent sensor for in vivo copper imaging in a murine Wilson disease model.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.13931984.15387095 ◽

2012 ◽

Author(s):

Janet Morrow

Keyword(s):

Wilson Disease ◽

Near Infrared ◽

Fluorescent Sensor ◽

Disease Model

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Faculty Opinions recommendation of cAMPr: A single-wavelength fluorescent sensor for cyclic AMP.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.732800686.793544098 ◽

2018 ◽

Author(s):

Ted Abel ◽

Mahesh Shetty

Keyword(s):

Cyclic Amp ◽

Fluorescent Sensor

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A New Fluorescent Salen-uranyl Sensor for the Sub-ppm Detection of Chemical Warfare Agents

Current Organic Chemistry ◽

10.2174/1385272824999200930150313 ◽

2020 ◽

Vol 24 (20) ◽

pp. 2378-2382

Author(s):

Andrea Pappalardo ◽

Chiara M.A. Gangemi ◽

Rosa Maria Toscano ◽

Giuseppe Trusso Sfrazzetto

Keyword(s):

Real Time ◽

Fluorescent Sensor ◽

Chemical Warfare Agents ◽

Optical Response ◽

Chemical Warfare ◽

Lethal Effects ◽

Strong Affinity ◽

Chemical Attack ◽

Fluorescence Measurements ◽

Warfare Agents

Real-time sensing of Chemical Warfare Agents (CWAs) is today a crucial topic to prevent the lethal effects of a terroristic chemical attack. For this reason, the development of efficient, selective, sensitive and reversible sensoristic devices, able to detect by optical response ppm levels of these compounds, is strongly required. Here, the synthesis of a new fluorescent sensor based on a salen-uranyl scaffold, functionalized with two bodipy moieties, and its application for the detection of sub-ppm levels of CWAs is reported. Detection properties were evaluated by fluorescence measurements and selectivity tests demonstrated the strong affinity for CWAs.

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