A fast method for in vitro biomineralization of PVA/alginate/biphasic calcium phosphate hydrogel

In the present study, an Intelligent Multi-parameter Simulated Evaluation in vitro （IMSE system） was used to study the deposition properties of apatite formation on the surface of biphasic calcium phosphate porous ceramic (BCP) from static and dynamic r-SBF. Results showed that apatite formed on the surface of BCP from static and dynamic r-SBF differed between each other. In static r-SBF, ions were transferred by diffusion, which could not compensate the consuming of calcium ions, and mist apatite layer was formed on the surface of samples. But in the dynamic r-SBF, simulated fluid was adjusted precisely and flowed forcedly, the concentrations of ions were homogeneous; with the compensation of ions, calcium and phosphate were supersaturated, and the free energy of apatite formation was negative, bone-like apatite sheets were formed on the surface of samples.

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Biocompatibility of Surface-Modified Biphasic Calcium Phosphate/Poly-L-Lactide Biocomposite in vitro and in vivo

Journal of Material Science and Technology ◽

10.1016/s1005-0302(10)60119-3 ◽

2010 ◽

Vol 26 (8) ◽

pp. 754-758 ◽

Cited By ~ 8

Author(s):

Weizhong Yang ◽

Guangfu Yin ◽

Dali Zhou ◽

Jianwen Gu ◽

Yadong Li ◽

...

Keyword(s):

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

Surface Modified

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In vitro evaluation of an injectable biphasic calcium phosphate (BCP) carrier system combined with recombinant human bone morphogenetic protein (rhBMP)-9

Bio-Medical Materials and Engineering ◽

10.3233/bme-171675 ◽

2017 ◽

Vol 28 (3) ◽

pp. 293-304 ◽

Cited By ~ 2

Author(s):

Masako Fujioka-Kobayashi ◽

Benoit Schaller ◽

Yufeng Zhang ◽

Benjamin E. Pippenger ◽

Richard J. Miron

Keyword(s):

Calcium Phosphate ◽

Bone Morphogenetic Protein ◽

Biphasic Calcium Phosphate ◽

Human Bone ◽

Carrier System ◽

In Vitro Evaluation ◽

Morphogenetic Protein ◽

Human Bone Morphogenetic Protein ◽

Recombinant Human Bone

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Development of injectable chitosan/biphasic calcium phosphate bone cement and in vitro and in vivo evaluation

Biomedical Materials ◽

10.1088/1748-605x/ab8441 ◽

2020 ◽

Vol 15 (5) ◽

pp. 055038

Author(s):

Sirirat T. Rattanachan ◽

Nuan La-ong Srakaew ◽

Paritat Thaitalay ◽

Oranich Thongsri ◽

Rawee Dangviriyakul ◽

...

Keyword(s):

Calcium Phosphate ◽

Bone Cement ◽

Biphasic Calcium Phosphate ◽

In Vivo Evaluation ◽

Calcium Phosphate Bone Cement

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Composite Bioceramics/Polymer Electrospun Scaffolds for Regenerative Medicine

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.529-530.441 ◽

2012 ◽

Vol 529-530 ◽

pp. 441-446

Author(s):

Thomas Miramond ◽

Pascal Borget ◽

Caroline Colombeix ◽

Serge Baroth ◽

G. Daculsi

Keyword(s):

Cell Adhesion ◽

Calcium Phosphate ◽

Regenerative Medicine ◽

Tricalcium Phosphate ◽

Biphasic Calcium Phosphate ◽

Electrospun Scaffolds ◽

Mineral Particles

The main goal of this study was to succeed in the relevant association of well-known osteoconductive biphasic calcium phosphate (BCP) made of Hydroxyapatite (20% HA) and β-Tricalcium Phosphate (80% β-TCP) crystallographic phases and resorbable poly (L-lactide-co-D,L-lactide)(PLDLLA) 3D matrices synthesized by electrospinning. Two types of mineral particles were obtained, BCP new hollow granules, and classical BCP particles. It appeared that hollow shells/PLDLLA composite 3D matrices allowed higher cell adhesionin vitro,thanks to internal concavities and are promising scaffolds in terms of cell carrying.

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A novel hybrid multichannel biphasic calcium phosphate granule-based composite scaffold for cartilage tissue regeneration

Journal of Biomaterials Applications ◽

10.1177/0885328217741757 ◽

2017 ◽

Vol 32 (6) ◽

pp. 775-787 ◽

Cited By ~ 5

Author(s):

Albert Jung ◽

Preeti Makkar ◽

Jhaleh Amirian ◽

Byong-Taek Lee

Keyword(s):

Compressive Strength ◽

Calcium Phosphate ◽

Biphasic Calcium Phosphate ◽

Composite System ◽

Composite Scaffold ◽

Cartilage Regeneration ◽

Cartilage Tissue ◽

In Vitro Biocompatibility ◽

Surface Modified

The objective of the present study was to develop a novel hybrid multichannel biphasic calcium phosphate granule (MCG)-based composite system for cartilage regeneration. First, hyaluronic acid-gelatin (HG) hydrogel was coated onto MCG matrix (MCG-HG). Poly(lactic-co-glycolic acid) (PLGA) microspheres was separately prepared and modified with polydopamine subsequent to BMP-7 loading (B). The surface-modified microspheres were finally embedded into MCG-HG scaffold to develop the novel hybrid (MCG-HG-PLGA-PD-B) composite system. The newly developed MCG-HG-PLGA-PD-B composite was then subjected to scanning electron microscopy, energy dispersive X-ray spectroscopy, Fourier Transform infrared spectroscopy, porosity, compressive strength, swelling, BMP-7 release and in-vitro biocompatibility studies. Results showed that 60% of BMP-7 retained on the granular surface after 28 days. A hybrid MCG-HG-PLGA-PD-B composite scaffold exhibited higher swelling and compressive strength compared to MCG-HG or MCG. In-vitro studies showed that MCG-HG-PLGA-PD-B had improved cell viability and cell proliferation for both MC3T3-E1 pre-osteoblasts and ATDC5 pre-chondrocytes cell line with respect to MCG-HG or MCG scaffold. Our results suggest that a hybrid MCG-HG-PLGA-PD-B composite scaffold can be a promising candidate for cartilage regeneration applications.

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