Abstract
This study attempts to clarify the basic material properties of in-vivo-fabricated hyaluronan (HA)/bacterial cellulose (BC) nanocomposites prepared previously. BC membranes (pellicles) generated by Gluconacetobacter hansenii (G. hansenii) are promising biomaterials owing to their outstanding biocompatible properties. Recently, specific demands for biomedical applications of BC have increased owing to its excellent mechanical properties. Although many techniques have been developed to improve the biofunctional properties of BC pellicles, such modifications remain limited owing to technical difficulties in the modulation of complex biosynthetic processes. Therefore, we previously developed an in vivo modification technique to produce nanocomposite pellicles composed of BC and HA (in vivo HA/BC), which are directly secreted from genetically engineered G. hansenii. In the present study, the HA extractability and content rate, physical characteristics, and cytocompatibility of in vivo HA/BC have been investigated in comparison to conventional in situ HA/BC and native BC pellicle. The results suggested that HA more strongly adsorbed to the solid BC surface of in vivo HA/BC than that of in situ HA/BC, which possibly affected the dynamic viscoelastic characteristics. Furthermore, in vivo HA/BC showed remarkably high human epidermal cell adhesion. These results indicate the great potential of in vivo modification to expand the usefulness of BC-based biomaterials.
Physical Characteristics and Cell-Adhesive Properties of In Vivo Fabricated Hyaluronan/Bacterial Cellulose Nanocomposites
