scholarly journals Physical Characteristics and Cell-Adhesive Properties of In Vivo Fabricated Hyaluronan/Bacterial Cellulose Nanocomposites

2021 ◽  
Author(s):  
Ryo Takahama ◽  
Honami Kato ◽  
Go Takayama ◽  
Kenji Tajima ◽  
Tetsuo Kondo
Keyword(s):  
Bacterial Cellulose ◽  
Physical Characteristics ◽  
Genetically Engineered ◽  
Basic Material ◽  
Adhesive Properties ◽  
Gluconacetobacter Hansenii ◽  
Modification Technique ◽  
Cellulose Nanocomposites

Abstract This study attempts to clarify the basic material properties of in-vivo-fabricated hyaluronan (HA)/bacterial cellulose (BC) nanocomposites prepared previously. BC membranes (pellicles) generated by Gluconacetobacter hansenii (G. hansenii) are promising biomaterials owing to their outstanding biocompatible properties. Recently, specific demands for biomedical applications of BC have increased owing to its excellent mechanical properties. Although many techniques have been developed to improve the biofunctional properties of BC pellicles, such modifications remain limited owing to technical difficulties in the modulation of complex biosynthetic processes. Therefore, we previously developed an in vivo modification technique to produce nanocomposite pellicles composed of BC and HA (in vivo HA/BC), which are directly secreted from genetically engineered G. hansenii. In the present study, the HA extractability and content rate, physical characteristics, and cytocompatibility of in vivo HA/BC have been investigated in comparison to conventional in situ HA/BC and native BC pellicle. The results suggested that HA more strongly adsorbed to the solid BC surface of in vivo HA/BC than that of in situ HA/BC, which possibly affected the dynamic viscoelastic characteristics. Furthermore, in vivo HA/BC showed remarkably high human epidermal cell adhesion. These results indicate the great potential of in vivo modification to expand the usefulness of BC-based biomaterials.

scholarly journals P12.01 Local implantation of thermoresponsive interferon alpha-polypeptide conjugate combined with temozolomide for post-surgical glioblastoma chemoimmunotherapy

2019 ◽  
Vol 21 (Supplement_3) ◽  
pp. iii59-iii59
Author(s):  
P Liang ◽  
W Zhuoran ◽  
G Weiping ◽  
W Guihuai
Keyword(s):  
Release Kinetics ◽  
Physicochemical Characterization ◽  
Antitumor Agent ◽  
Genetically Engineered ◽  
Resection Cavity ◽  
Post Surgery ◽  
Zero Order Release ◽  
Immunosuppressive Microenvironment

Abstract BACKGROUND Glioblastoma(GBM) is associated with gloomy prognosis despite maximal safe resection and following chemoradiation. In recent years, significant progress has been made in cancer immunotherapy except for GBM, which is largerly due to its local immunosuppressive microenvironment. Some research has demonstrated that local immunotherapy may be more efficient than systematic administrators. IFNα has been investigated as antitumor agent for some cancers, including glioma. Nevertheless, the short circulating half-life greatly limits its clinical application. Hence, a thermoresponsive IFNα-elastin-like polypeptide (IFNα-ELP) was genetically engineered for the earliest local intervention post-surgery. MATERIAL AND METHODS Firstly, IFNα-ELP(V) was constructed, expressed and purified, then, its physicochemical characterization was verified. The tumor was resected 10 days after U87MG-mCherry-luc cells orthotopic implantation, and IFNα-ELP(V) was injected into the resection cavity. Two days later, temozolomide(TMZ) was intraperitoneally injected. Using in vivo imaging technique, we could monitor the trends in tumor size. The survival time of mice was counted. Biosafety was evaluated by peripheral blood biochemistry analysis and pathology of the organs. RESULTS In this study, the bioconjugate not only in situ deposited in the resection cavity because of the thermoresponsive characteristic, but also showed zero-order release kinetics from the depot and dramatically improved pharmacokinetics and biodistribution of IFNα. Consequently, it showed the inhibition of tumor relapse in GBM orthotopic resection mice models. When followed by TMZ intraperitoneal injection, IFNα-ELP(V) could significantly prevent the tumor recurrence than itself or TMZ alone. Biosafety results indicated that the systemic toxicity of IFNα-ELP(V) in mice can be reduced to safe levels. CONCLUSION The results reveal that local implantation of thermoresponsive IFNα-ELP(V) combined with TMZ exhibits the synergy of post-surgical GBM chemoimmunotherapy.

scholarly journals Probiotic Associated Therapeutic Curli Hybrids (PATCH)

2018 ◽  
Author(s):  
Pichet Praveschotinunt ◽  
Anna M. Duraj-Thatte ◽  
Ilia Gelfat ◽  
Franziska Bahl ◽  
David B. Chou ◽  
...  
Keyword(s):  
Barrier Function ◽  
Unmet Need ◽  
Intestinal Barrier ◽  
Genetically Engineered ◽  
Intestinal Barrier Function ◽  
Beneficial Bacteria ◽  
Fibrous Matrix

AbstractThere is an unmet need for new treatment methods for inflammatory bowel disease (IBD) that can reliably maintain remission without leading to detrimental side effects. Beneficial bacteria have been utilized as an alternative treatment for IBD albeit with low efficacy. We genetically engineered Escherichia coli Nissle 1917 (EcN) to create an anti-inflammatory fibrous matrix in situ. This matrix consists of EcN-produced curli nanofibers displaying trefoil factors (TFFs), known to promote intestinal barrier function and epithelial restitution. We confirmed that engineered EcN was able to secrete the curli-fused TFFs in vitro and in vivo, and was non-pathogenic. We observed an enhanced protective effect of engineered EcN against dextran sodium sulfate induced colitis in mice, associated with barrier function reinforcement and immunomodulation. This work sets the foundation for the development of a novel therapeutic platform in which the in situ production of a therapeutic protein matrix from beneficial bacteria can be exploited.

Preparation and Properties of Polystyrene/Bacterial Cellulose Nanocomposites by In Situ Polymerization

2011 ◽  
Vol 50 (10) ◽  
pp. 1921-1927 ◽  
Author(s):  
Kun Peng ◽  
Biao Wang ◽  
Shiyan Chen ◽  
Chunyan Zhong ◽  
Huaping Wang
Keyword(s):  
Bacterial Cellulose ◽  
In Situ Polymerization ◽  
Cellulose Nanocomposites

Effect of pandan extract concentration to chromium (IV) removal using bacterial cellulose-pandan composites prepared by in-situ modification technique

2020 ◽  
Vol 31 ◽  
pp. 89-95
Author(s):  
Ida Idayu Muhamad ◽  
Siti Nur Hidayah Muhamad ◽  
Mohd Harfiz Salehudin ◽  
Khairul Azly Zahan ◽  
Woei Yenn Tong ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Extract Concentration ◽  
In Situ Modification ◽  
Modification Technique

Antimicrobial bacterial cellulose nanocomposites prepared by in situ polymerization of 2-aminoethyl methacrylate

2015 ◽  
Vol 123 ◽  
pp. 443-453 ◽  
Author(s):  
Ana R.P. Figueiredo ◽  
Andrea G.P.R. Figueiredo ◽  
Nuno H.C.S. Silva ◽  
Ana Barros-Timmons ◽  
Adelaide Almeida ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
In Situ Polymerization ◽  
Cellulose Nanocomposites

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

Three-Dimensional Subcellular Organization of Hepatocytes in Intact Liver: Simultaneous Visualization of Cytoskeletal and Membrane Markers by Confocal Microscopy

1996 ◽  
Vol 54 ◽  
pp. 288-289
Author(s):  
Greg V. Martin ◽  
Ann L. Hubbard
Keyword(s):  
Three Dimensional ◽  
Integral Membrane Proteins ◽  
Polarized Secretion ◽  
Microscope Images ◽  
Computer Aided ◽  
Intracellular Organelles ◽  
Cytoskeletal Elements ◽  
Simultaneous Visualization

The microtubule (MT) cytoskeleton is necessary for many of the polarized functions of hepatocytes. Among the functions dependent on the MT-based cytoskeleton are polarized secretion of proteins, delivery of endocytosed material to lysosomes, and transcytosis of integral plasma membrane (PM) proteins. Although microtubules have been shown to be crucial to the establishment and maintenance of functional and structural polarization in the hepatocyte, little is known about the architecture of the hepatocyte MT cytoskeleton in vivo, particularly with regard to its relationship to PM domains and membranous organelles. Using an in situ extraction technique that preserves both microtubules and cellular membranes, we have developed a protocol for immunofluorescent co-localization of cytoskeletal elements and integral membrane proteins within 20 µm cryosections of fixed rat liver. Computer-aided 3D reconstruction of multi-spectral confocal microscope images was used to visualize the spatial relationships among the MT cytoskeleton, PM domains and intracellular organelles.

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