A multiplex PCR for detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis in clinical specimens

Molecular and Cellular Probes ◽

10.1016/j.mcp.2005.05.002 ◽

2005 ◽

Vol 19 (5) ◽

pp. 314-322 ◽

Author(s):

Erin A. McDonough ◽

Christopher P. Barrozo ◽

Kevin L. Russell ◽

David Metzgar

Keyword(s):

Multiplex Pcr ◽

Mycoplasma Pneumoniae ◽

Bordetella Pertussis ◽

Legionella Pneumophila ◽

Chlamydophila Pneumoniae ◽

Clinical Specimens

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A Multiplex PCR for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis in Clinical Specimens

10.21236/ada432554 ◽

2005 ◽

Author(s):

E. A. McDonough ◽

C. P. Barrozo ◽

K. L. Russell ◽

D. Metzgar

Keyword(s):

Multiplex Pcr ◽

Mycoplasma Pneumoniae ◽

Bordetella Pertussis ◽

Legionella Pneumophila ◽

Chlamydophila Pneumoniae ◽

Clinical Specimens

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The Pneumoplex Assays, a Multiplex PCR-Enzyme Hybridization Assay That Allows Simultaneous Detection of Five Organisms, Mycoplasma pneumoniae, Chlamydia (Chlamydophila) pneumoniae, Legionella pneumophila, Legionella micdadei, and Bordetella pertussis, and Its Real-Time Counterpart

Journal of Clinical Microbiology ◽

10.1128/jcm.43.2.565-571.2005 ◽

2005 ◽

Vol 43 (2) ◽

pp. 565-571 ◽

Author(s):

M. Khanna ◽

J. Fan ◽

K. Pehler-Harrington ◽

C. Waters ◽

P. Douglass ◽

...

Keyword(s):

Multiplex Pcr ◽

Real Time ◽

Mycoplasma Pneumoniae ◽

Bordetella Pertussis ◽

Legionella Pneumophila ◽

Simultaneous Detection ◽

Chlamydophila Pneumoniae ◽

Hybridization Assay ◽

Legionella Micdadei

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A Single-Tube Multiple-Locus Variable-Number Tandem-Repeat Analysis of Mycoplasma pneumoniae Clinical Specimens by Use of Multiplex PCR-Capillary Electrophoresis

Journal of Clinical Microbiology ◽

10.1128/jcm.02178-14 ◽

2014 ◽

Vol 52 (12) ◽

pp. 4168-4171 ◽

Author(s):

C. Yan ◽

H. Sun ◽

G. Xue ◽

H. Zhao ◽

L. Wang ◽

...

Keyword(s):

Capillary Electrophoresis ◽

Multiplex Pcr ◽

Mycoplasma Pneumoniae ◽

Tandem Repeat ◽

Variable Number Tandem Repeat ◽

Variable Number ◽

Single Tube ◽

Clinical Specimens ◽

Multiple Locus ◽

Repeat Analysis

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Diagnóstico molecular de neumonías bacterianas atípicas provenientes de la comunidad. Paraguay (2014-2017)

Revista de la Sociedad Científica del Paraguay ◽

10.32480/rscp.2018-23-1.139-146 ◽

2018 ◽

Vol 23 (1) ◽

pp. 139-146

Author(s):

María E. León ◽

Aníbal Kawabata ◽

Minako Nagai ◽

Liliana Rojas ◽

Gustavo Chamorro

Keyword(s):

Mycoplasma Pneumoniae ◽

Legionella Pneumophila ◽

Chlamydophila Pneumoniae ◽

Objetivos: Determinar la frecuencia de neumonías bacterianas atípicas causadas por Legionella pneumophila, Mycoplasma pneumoniae y Chlamydophila pneumoniae, en niños y adultos de Paraguay durante el periodo 2014-2017 por técnicas moleculares. Materiales y métodos: se incluyeron en este estudio 148 muestras de líquido pleural de niños y adultos con diagnóstico de neumonía adquirida en la comunidad (NAC) durante el periodo 2014 a febrero de 2017. Las muestras fueron remitidas al Laboratorio Central de Salud Pública por los Centros Centinelas y Centros Colaboradores de la Red de Vigilancia de Meningitis y Neumonías. Resultados: La frecuencia general de neumonías atípicas resultó 1.4 % (2/148). Se detectó Legionella pneumophila en 0.7% (1/148) de los pacientes con sospecha de neumonía y un caso Mycoplasma pneumoniae en 0.7% (1/148). Conclusión: Los agentes atípicos presentan manifestaciones inespecíficas y variables de un caso a otro, haciéndolas difíciles de distinguir de otras etiologías. Los nuevos enfoques del diagnóstico microbiológico son prometedores, se espera que las pruebas de diagnóstico molecular para estos patógenos sean importantes herramientas en el laboratorio clínico.

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Detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae directly from respiratory clinical specimens using a rapid real-time polymerase chain reaction assay

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2012.03.024 ◽

2012 ◽

Vol 73 (3) ◽

pp. 278-280 ◽

Author(s):

Maureen H. Diaz ◽

Jonas M. Winchell

Keyword(s):

Polymerase Chain Reaction ◽

Real Time ◽

Mycoplasma Pneumoniae ◽

Polymerase Chain Reaction Assay ◽

Chlamydophila Pneumoniae ◽

Chain Reaction ◽

Clinical Specimens ◽

Polymerase Chain

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Development and Evaluation of Chlamylege, a New Commercial Test Allowing Simultaneous Detection and Identification of Legionella, Chlamydophila pneumoniae, and Mycoplasma pneumoniae in Clinical Respiratory Specimens by Multiplex PCR

Journal of Clinical Microbiology ◽

10.1128/jcm.43.7.3247-3254.2005 ◽

2005 ◽

Vol 43 (7) ◽

pp. 3247-3254 ◽

Author(s):

C. Ginevra ◽

C. Barranger ◽

A. Ros ◽

O. Mory ◽

J.-L. Stephan ◽

...

Keyword(s):

Multiplex Pcr ◽

Mycoplasma Pneumoniae ◽

Simultaneous Detection ◽

Chlamydophila Pneumoniae ◽

Commercial Test ◽

Detection And Identification ◽

Respiratory Specimens

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Multiplex PCR for the simultaneous detection of Chlamydia pneumoniae, Mycoplasma pneumoniae and Legionella pneumophila in community-acquired pneumonia

Respiratory Medicine ◽

10.1016/j.rmed.2003.11.012 ◽

2004 ◽

Vol 98 (6) ◽

pp. 542-550 ◽

Author(s):

Naoyuki Miyashita ◽

Atsushi Saito ◽

Shigeru Kohno ◽

Keizo Yamaguchi ◽

Akira Watanabe ◽

...

Keyword(s):

Multiplex Pcr ◽

Mycoplasma Pneumoniae ◽

Legionella Pneumophila ◽

Chlamydia Pneumoniae ◽

Simultaneous Detection ◽

Community Acquired Pneumonia

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Predicting Mycoplasma pneumoniae and Chlamydophila pneumoniae in community-acquired pneumonia (CAP) pneumonia: epidemiological study of respiratory tract infection using multiplex PCR assays

Internal and Emergency Medicine ◽

10.1007/s11739-021-02744-6 ◽

2021 ◽

Author(s):

Naoto Ishimaru ◽

Satoshi Suzuki ◽

Toshio Shimokawa ◽

Yusaku Akashi ◽

Yuto Takeuchi ◽

...

Keyword(s):

Tract Infection ◽

Respiratory Tract ◽

Respiratory Tract Infection ◽

Multiplex Pcr ◽

Epidemiological Study ◽

Mycoplasma Pneumoniae ◽

Chlamydophila Pneumoniae ◽

Community Acquired Pneumonia ◽

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Undiagnosed Bordetella pertussis among Chlamydophila pneumoniae and Mycoplasma pneumoniae patient samples – a pilot study

10.26226/morressier.56d5ba25d462b80296c94a02 ◽

2016 ◽

Author(s):

Karolina Gullsby

Keyword(s):

Pilot Study ◽

Mycoplasma Pneumoniae ◽

Bordetella Pertussis ◽

Chlamydophila Pneumoniae

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Comparison of Sputum and Nasopharyngeal Swab Specimens for Molecular Diagnosis of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila

Annals of Laboratory Medicine ◽

10.3343/alm.2012.32.2.133 ◽

2012 ◽

Vol 32 (2) ◽

pp. 133-138 ◽

Author(s):

Min-Chul Cho ◽

Hyewon Kim ◽

Dongheui An ◽

Miyoung Lee ◽

Shin-Ae Noh ◽

...

Keyword(s):

Mycoplasma Pneumoniae ◽

Molecular Diagnosis ◽

Legionella Pneumophila ◽

Chlamydophila Pneumoniae ◽

Nasopharyngeal Swab

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