DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, Prospects and Limitations

2021 ◽  
pp. 101758
Author(s):  
Ibrahim Khalil ◽  
Abu Hashem ◽  
Amit R. Nath ◽  
Nurhidayatullaili Muhd Julkapli ◽  
Wageeh A. Yehye ◽  
...  
Keyword(s):  
Genetic Detection

scholarly journals Effects of Inbreeding on Genetic Characteristic, Growth, Survival Rates, and Immune Responses of a New Inbred Line of Exopalaemon carinicauda

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Jiajia Wang ◽  
Jitao Li ◽  
Qianqian Ge ◽  
Zhao Chen ◽  
Jian Li
Keyword(s):  
Immune Responses ◽  
Inbred Line ◽  
Survival Rates ◽  
The Body ◽  
Exopalaemon Carinicauda ◽  
Economic Traits ◽  
Gene Frequencies ◽  
Research Fields ◽  
Ssr Loci ◽  
Genetic Detection

The Exopalaemon carinicauda could be a useful crustacean laboratory animal in many research fields. We newly established an inbred line of Exopalaemon carinicauda named EC4 inbred line by brother×sister mating and keeping to F11 generation. Trends in heterozygosity in the process of producing EC4 inbred line were examined through the characterization of polymorphisms based on gene frequencies of SNP and EST-SSR loci. The results demonstrated that the number of alleles (N), observed heterozygosity (Ho), expected heterozygosity (He), and polymorphism information content (PIC) gradually decreased with the increase of inbreeding generations. The genetic detection results indicated that 9 (29.03%, 9/31) of the SNP loci and 15 (32.61%, 15/46) of the EST-SSR loci were homozygous in F11 generation of EC4 inbred line. The variation of the growth-related traits, the immune responses, and antioxidant status were described in experimental full-sibling inbred populations of E. carinicauda at five levels of inbreeding coefficient (F=0.785, F=0.816, F=0.859, F=0.886, F=0.908) under controlled laboratory conditions. The body weight, body length, and survival rate in EC4 inbred line of all generations were less than the control population. Inbreeding affected the antibacterial activity, phenoloxidase (PO) activity, and superoxide dismutase (SOD) which decreased at the eleventh generation of EC4 inbred line. This study demonstrated that inbreeding had a negative effect on the economic traits and immune response, but our inbred line was established successfully until F11 and confirmed by genetic detection using SNP and EST-SSR loci.

Genetic detection and characterization of goose parvovirus: Implications for epidemiology and pathogenicity in Cherry Valley Pekin ducks

2017 ◽  
Vol 51 ◽  
pp. 101-103 ◽  
Author(s):  
Kang Ning ◽  
Te Liang ◽  
Minghang Wang ◽  
Yunhan Dong ◽  
Shenghua Qu ◽  
...  
Keyword(s):  
Pekin Ducks ◽  
Goose Parvovirus ◽  
Genetic Detection

Molecular genetic detection of Xp21 muscular dystrophy carriers in Cyprus

1994 ◽  
Vol 48 (8-9) ◽  
pp. 355-358 ◽  
Author(s):  
K. Christodoulou ◽  
P. Ioannou ◽  
L. Middleton
Keyword(s):  
Muscular Dystrophy ◽  
Molecular Genetic ◽  
Genetic Detection

scholarly journals Identification of a De Novoc.1000delA ANK1 mutation associated to hereditary spherocytosis in a neonate with Coombs-negative hemolytic jaundice-case reports and review of the literature 

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Lichun Xie ◽  
Zhihao Xing ◽  
Changgang Li ◽  
Si-xi Liu ◽  
Fei-qiu Wen
Keyword(s):  
De Novo ◽  
Hereditary Spherocytosis ◽  
Case Reports ◽  
De Novo Mutation ◽  
Case Presentation ◽  
Important Means ◽  
Genetic Detection ◽  
Membrane Defects ◽  
Severe Jaundice ◽  
Neonates And Infants

Abstract Background To strengthen the understanding of Hereditary Spherocytosis (HS) and determine the disease-causing mutation present with neonatal jaundice. HS is a hemolytic condition resulting from various erythrocyte membrane defects. Many different mutations result in HS, including mutations in ANK1. Case presentation A term neonate presented at ten hours with severe jaundice requiring exchange transfusion. At two months he was hospitalized due to repeated pallor and anemia requiring blood transfusions. Using next-generation sequencing, we discovered the responsible mutation in the proband but not in his parents; a heterozygous nucleotide variation of c.1000delA (p.1334Sfs*6) in ANK1. Thus hereditary spherocytosis was diagnosed. Conclusions Genetic detection is an important means of discovering the cause of hemolytic anemia in neonates and infants where routine diagnostic tests are unrevealing. We found a novel de novo mutation, c.1000delA (p.1334Sfs*6) in ANK1 that might account for other cases of HS in the Chinese population.

scholarly journals A case of death within 72 hours after community-acquired bloodstream infection caused by klebsiella variicola

2021 ◽  
Author(s):  
Dali Long ◽  
Yuhui Wang ◽  
Jinlong Wang ◽  
Sijie Mu ◽  
Li Chen ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Rare Case ◽  
Bacterial Culture ◽  
Drug Susceptibility ◽  
Klebsiella Pneumonia ◽  
Gene Detection ◽  
Laboratory Methods ◽  
Short Period ◽  
Genetic Detection ◽  
Illness Progression

Abstract Klebsiella subspecies infection is widely misdiagnosed and underestimated in clinic. We report a patient who was admitted to hospital due to unknown high fever. Bacterial culture only revealed the klebsiella pneumonia. Broad-spectrum antibiotics failed to improve patient's symptoms, although these antibiotics are sensitive to klebsiella pneumonia according to the drug susceptibility results. Patient rapidly entered sepsis and subsequent sepsis shock, and died within 72 hours. The delayed PMseq-DNA Pro high throughput gene detection revealed the mixed infection of klebsiella pneumoniae and klebsiella variicola. This is a very rare case that patient suffered so rapid deterioration and died from bacterial infection within short period of time. Klebsiella variicola could contribute to rapid illness progression, while it was revealed by gene detection rather than classic laboratory methods. Which suggests that early genetic detection should be recommended in cases of complex infection.

scholarly journals HUMAN PAPILLOMAVIRUS AS AN EPIGENETIC COFACTOR OF THE DEVELOPMENT OF SEVERAL EPITHELIAL NON-MELANOCYTIC SKIN NEOPLASMS (LITERATURE REVIEW)

2019 ◽  
Vol 22 (5-6) ◽  
pp. 138-148
Author(s):  
Avad Zhaber Mahmud Zhaber ◽  
E. S Snarskaya
Keyword(s):  
Human Papillomavirus ◽  
Molecular Genetic ◽  
Cumulative Effect ◽  
Skin Neoplasms ◽  
Biological Data ◽  
Detection Methods ◽  
Human Papillomavirus Infection ◽  
Papillomavirus Infection ◽  
Genetic Detection

In recent decades, interest in the role of human papillomavirus (HPV) has been steadily increasing, which can be attributed both to the evolution of molecular genetic detection methods and to the widespread of this viral infection in the population. Epidemiological and molecular biological data suggest that HPV genus beta can cause the development of a number of epithelial non-melanocytic neoplasms of the skin. However, this relationship has not yet been fully studied. Possibly, human papillomavirus infection should be considered from the perspective of co-carcinogenesis with the cumulative effect of UV irradiation, which is indirectly indicated by the predominant localization of elements in open areas of the skin and the high risks of their malignant transformation.

scholarly journals Genetic Detection of Circulating Cancer Cells and Micrometastasis in the Lymph Node.

1997 ◽  
Vol 30 (4) ◽  
pp. 891-896
Author(s):  
Shoji Nakamori ◽  
Masao Kameyama ◽  
Shingi Imaoka ◽  
Takushi Yasuda ◽  
Toshiyuki Kabuto ◽  
...  
Keyword(s):  
Lymph Node ◽  
Cancer Cells ◽  
Circulating Cancer Cells ◽  
Genetic Detection

scholarly journals Detection and Discrimination of Cryptosporidium parvum and C. hominis in Water Samples by Immunomagnetic Separation-PCR

2005 ◽  
Vol 71 (2) ◽  
pp. 898-903 ◽  
Author(s):  
Yoshitsugu Ochiai ◽  
Chieko Takada ◽  
Mitsugu Hosaka
Keyword(s):  
Cryptosporidium Parvum ◽  
Water Samples ◽  
Nested Pcr ◽  
Fragment Length Polymorphism ◽  
Detection Method ◽  
Fluorescent Antibody ◽  
Immunomagnetic Separation ◽  
Antibody Assay ◽  
Pcr Products ◽  
Genetic Detection

ABSTRACT Cryptosporidium parvum and C. hominis have been the cause of large and serious outbreaks of waterborne cryptosporidiosis. A specific and sensitive recovery-detection method is required for control of this pathogen in drinking water. In the present study, nested PCR-restriction fragment length polymorphism (RFLP), which targets the divergent Cpgp40/15 gene, was developed. This nested PCR detected only the gene derived from C. parvum and C. hominis strains, and RFLP was able to discriminate between the PCR products from C. parvum and C. hominis. To evaluate the sensitivity of nested PCR, C. parvum oocysts inoculated in water samples of two different turbidities were recovered by immunomagnetic separation (IMS) and detected by nested PCR and fluorescent antibody assay (FA). Genetic detection by nested PCR and oocyst number confirmed by FA were compared, and the results suggested that detection by nested PCR depends on the confirmed oocyst number and that nested PCR in combination with IMS has the ability to detect a single oocyst in a water sample. We applied an agitation procedure with river water solids to which oocysts were added to evaluate the recovery and detection by the procedure in environmental samples and found some decrease in the rate of detection by IMS.

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