The fluorescent probe-based recombinase-aided amplification for rapid detection of Escherichia coli O157:H7

2021 ◽  
pp. 101777
Author(s):  
Dan Mu ◽  
Donggen Zhou ◽  
Guoyang Xie ◽  
Ju Liu ◽  
Qing Xiong ◽  
...  
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.


2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Ling Wang ◽  
Ronghui Wang ◽  
Byung-Whi Kong ◽  
Sha Jin ◽  
Kaiming Ye ◽  
...  

2007 ◽  
Vol 584 (1) ◽  
pp. 66-71 ◽  
Author(s):  
Lisa C. Shriver-Lake ◽  
Stephanie Turner ◽  
Chris R. Taitt

2019 ◽  
Vol 13 (2) ◽  
pp. 474-481 ◽  
Author(s):  
Zhenbo Xu ◽  
Yuting Luo ◽  
Thanapop Soteyome ◽  
Chii-Wann Lin ◽  
Xingyong Xu ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document