Genetic variability of the measles virus hemagglutinin gene in B3 genotype strains circulating in Northern Italy

2019 ◽  
Vol 75 ◽  
pp. 103943 ◽  
Author(s):  
G. Ciceri ◽  
M. Canuti ◽  
S. Bianchi ◽  
M. Gori ◽  
A. Piralla ◽  
...  
2020 ◽  
Vol 21 (12) ◽  
pp. 4435
Author(s):  
Silvia Bianchi ◽  
Marta Canuti ◽  
Giulia Ciceri ◽  
Maria Gori ◽  
Daniela Colzani ◽  
...  

Of the 24 known measles genotypes, only D8 and B3 are responsible for outbreaks in the last years in Europe, Asia, and America. In this study the H gene of 92 strains circulating between 2015 and 2019 in Lombardy, Northern Italy, and 1273 H sequences available in GenBank were analyzed in order to evaluate the genetic variability and to assess the conservation of the immunodominant sites. Overall, in Lombardy we observed the presence of four different B3 and three different D8 clusters, each one of them including sequences derived from viruses found in both vaccinated and unvaccinated subjects. Worldwide, the residue 400 within the H protein, a position located within the main immune epitope, is mutated in all circulating strains that belong to the two globally endemic genotypes, B3 and D8. Our data demonstrate the usefulness of measles virus (MV) H gene sequencing. Indeed, the monitoring the H protein epitopes of circulating strains could be included in the measles laboratory surveillance activities in order to improve and optimize strategies for measles control, as countries go towards elimination phase.


2009 ◽  
Vol 90 (12) ◽  
pp. 2877-2883 ◽  
Author(s):  
Giovanna Carpi ◽  
Luigi Bertolotti ◽  
Sergio Rosati ◽  
Annapaola Rizzoli

Tick-borne encephalitis (TBE) is a severe disease that has been endemic in north-east Italy since 1992. Over the past two decades, there has been an increase in the number of human cases reported in many European countries, including Italy. To assess the current TBE infection risk, questing ticks were collected from known TBE foci, as well as from a site in northern Italy where no human infections have been reported previously. A total of 1739 Ixodes ricinus (1485 nymphs and 254 adults) was collected and analysed for TBEV prevalence by a real-time RT-PCR targeting the 3′ untranslated region. Phylogenetic analyses of the partial envelope gene were conducted on two newly sequenced TBE virus (TBEV) strains and 28 previously published sequences to investigate the genealogical relationships of the circulating TBEV strains. These phylogenetic analyses confirmed a previous report that the European TBEV subtype is the only subtype circulating within the TBE foci in north-east Italy. Interestingly, nucleotide sequence analysis revealed a high degree of divergence (mean 2.54 %) between the TBEV strains recovered in the Italian province of Trento, despite the circulation of a single TBEV subtype. This elevated genetic variability within a single TBE focus may reflect local differences in the long-standing evolutionary dynamics of TBEV at this site relative to previously characterized sites, or more recent and continuous reintroduction of various TBEV strains.


Genes ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 1409
Author(s):  
Kyriaki Koupadi ◽  
Francesco Fontani ◽  
Marta Maria Ciucani ◽  
Elena Maini ◽  
Sara De Fanti ◽  
...  

Dog domestication is still largely unresolved due to time-gaps in the sampling of regions. Ancient Italian canids are particularly understudied, currently represented by only a few specimens. In the present study, we sampled 27 canid remains from Northern Italy dated between the Late Pleistocene and Bronze Age to assess their genetic variability, and thus add context to dog domestication dynamics. They were targeted at four DNA fragments of the hypervariable region 1 of mitochondrial DNA. A total of 11 samples had good DNA preservation and were used for phylogenetic analyses. The dog samples were assigned to dog haplogroups A, C and D, and a Late Pleistocene wolf was set into wolf haplogroup 2. We present our data in the landscape of ancient and modern dog genetic variability, with a particular focus on the ancient Italian samples published thus far. Our results suggest there is high genetic variability within ancient Italian canids, where close relationships were evident between both a ~24,700 years old Italian canid, and Iberian and Bulgarian ancient dogs. These findings emphasize that disentangling dog domestication dynamics benefits from the analysis of specimens from Southern European regions.


2017 ◽  
Vol 4 (1) ◽  
pp. 105
Author(s):  
Daniele Paganelli ◽  
Alessandra Pandolfi ◽  
Renato Sconfietti

In this short communication, we report the unusual presence of blind specimens of the non-native <em>Gammarus</em> <em>roeselii</em> Gervais 1835 (Amphioda, Gammaridae) in the sub-lacustrine Ticino River basin (Po River floodplain, Northern Italy). Considering that <em>G</em>. <em>roeselii</em> is present in almost all small semi-natural tributaries of the Ticino River, it is important to carry out further research on this well-established exotic species to verify its genetic variability.


2016 ◽  
Vol 81 ◽  
pp. 34-42 ◽  
Author(s):  
Giulia Piccirilli ◽  
Angela Chiereghin ◽  
Maria Grazia Pascucci ◽  
Gabriella Frasca ◽  
Roberta Zuntini ◽  
...  

2006 ◽  
Vol 6 (4) ◽  
pp. 269-276 ◽  
Author(s):  
Mirjam Kühne ◽  
David W.G. Brown ◽  
Li Jin

2015 ◽  
Vol 213 (4) ◽  
pp. 592-599 ◽  
Author(s):  
Heli Harvala ◽  
Åsa Wiman ◽  
Anders Wallensten ◽  
Katherina Zakikhany ◽  
Hélène Englund ◽  
...  

1995 ◽  
Vol 35 (1) ◽  
pp. 1-16 ◽  
Author(s):  
Tetsuo Nakayama ◽  
Takayuki Mori ◽  
Shinya Yamaguchi ◽  
Satomi Sonoda ◽  
Sinnji Asamura ◽  
...  

Apmis ◽  
2000 ◽  
Vol 108 (4) ◽  
pp. 267-272 ◽  
Author(s):  
LOTTE DAHL ◽  
LAURIDS SIIG CHRISTENSEN ◽  
SUSANNE SCHOELLER ◽  
HENRIK WESTH ◽  
ANNE-MARIE PLESNER

Sign in / Sign up

Export Citation Format

Share Document