Inflammatory responses of a macrophage/epithelial cell co-culture model to mono and mixed infections with Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia

2006 ◽  
Vol 8 (1) ◽  
pp. 27-35 ◽  
Author(s):  
Charles Bodet ◽  
Fatiha Chandad ◽  
Daniel Grenier
Keyword(s):  
Epithelial Cell ◽  
Porphyromonas Gingivalis ◽  
Inflammatory Responses ◽  
Treponema Denticola ◽  
Mixed Infections ◽  
Tannerella Forsythia ◽  
Culture Model

Presence of archaea and selected bacteria in infected root canal systems

2018 ◽  
Vol 64 (5) ◽  
pp. 317-326 ◽  
Author(s):  
Ewa Brzezińska-Błaszczyk ◽  
Elżbieta Pawłowska ◽  
Tomasz Płoszaj ◽  
Henryk Witas ◽  
Urszula Godzik ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Porphyromonas Gingivalis ◽  
Root Canal ◽  
Expression Profiles ◽  
Rna Extraction ◽  
Treponema Denticola ◽  
Rrna Gene ◽  
Pulp Tissue ◽  
Tannerella Forsythia

Infections of the root canal have polymicrobial etiology. The main group of microflora in the infected pulp is bacteria. There is limited data that archaea may be present in infected pulp tissue. The aim of this study was to check the prevalence of archaea in necrotic root canal samples obtained from patients with primary or post-treatment infection. The prevalence of selected bacteria species (Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Synergistes sp.) in necrotic samples was evaluated as well. Sixty-four samples from root canal were collected for DNA and RNA extraction. A PCR assay based on the 16S rRNA gene was used to determine the presence of archaea and selected bacteria. Of the 64 samples, 6 were analyzed by semiquantitative reverse transcription PCR to estimate expression profiles of 16S rRNA, and another 9 were selected for direct sequencing. Archaea were detected in 48.4% samples. Statistical analysis indicated a negative association in coexistence between archaea and Treponema denticola (P < 0.05; Pearson’s χ2 test). The main representative of the Archaea domain found in infected pulp tissue was Methanobrevibacter oralis. Archaea 16S rRNA gene expression was significantly lower than Synergistes sp., Porphyromonas gingivalis, and Tannerella forsythia (P < 0.05; Student’s t test). Thus, it can be hypothesized that archaea may participate in the endodontic microbial community.

scholarly journals Mixed Red-Complex Bacterial Infection in Periodontitis

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
N. Suzuki ◽  
M. Yoneda ◽  
T. Hirofuji
Keyword(s):  
Periodontal Disease ◽  
Bacterial Infection ◽  
Porphyromonas Gingivalis ◽  
Treponema Denticola ◽  
Periodontal Tissue ◽  
Tannerella Forsythia

The red complex, which includesPorphyromonas gingivalis,Treponema denticola, andTannerella forsythia(formerlyBacteroides forsythus), are recognized as the most important pathogens in adult periodontal disease. These bacteria are usually found together in periodontal pockets, suggesting that they may cause destruction of the periodontal tissue in a cooperative manner. This article discusses the interspecies pathogenic interactions within the red complex.

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