Mixed Red-Complex Bacterial Infection in Periodontitis

Treponema Denticola ◽

Periodontal Tissue ◽

The red complex, which includesPorphyromonas gingivalis,Treponema denticola, andTannerella forsythia(formerlyBacteroides forsythus), are recognized as the most important pathogens in adult periodontal disease. These bacteria are usually found together in periodontal pockets, suggesting that they may cause destruction of the periodontal tissue in a cooperative manner. This article discusses the interspecies pathogenic interactions within the red complex.

Relative quantification of Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia and Aggregatibacter actinomycetemcomitans high-risk bacterial species in Romanian patients evaluated for periodontal disease

10.1101/184770 ◽

2017 ◽

Author(s):

Cosac Ion Constantin ◽

Ionica Consuel ◽

Ratiu Attila Cristian ◽

Savu Lorand

Keyword(s):

Periodontal Disease ◽

Gingival Crevicular Fluid ◽

Bacterial Species ◽

Bacterial Load ◽

Clinical Signs ◽

Aggregatibacter Actinomycetemcomitans ◽

Treponema Denticola ◽

Tannerella Forsythia ◽

Complex Species

AbstractThree bacterial species pertaining to the red complex (Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia) and Aggregatibacter actinomycetemcomitans were investigated in relation to the incidence and severity of periodontal disease. A total of 259 patients were included in this study, 179 being diagnosed with periodontal disease. The gingival crevicular fluid samples were obtained from periodontal pockets and the presence and levels of target bacteria were assessed following DNA extraction and real-time quantitative PCR. Our results account for significant positive associations between the number of bacterial species from the red complex coexisting within a patient and several clinical signs (gingival bleeding, inflammation and bone deterioration). A similar positive association was found between bacterial load of the red complex species and the clinical Case Type diagnostic of the periodontal disease, as well as the probing depth with the most evident results for T. denticola. In conclusion, our study, a first for the Romanian population, confirms previous results found elsewhere and finds a possible regional pathogenic specificity for T. denticola as a major factor for periodontitis severity.

Chitosan gel prevents the growth of Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola in mini-implant during orthodontic treatment

The Saudi Dental Journal ◽

10.1016/j.sdentj.2021.06.003 ◽

2021 ◽

Author(s):

Haru Setyo Anggani ◽

Victoria Rusli ◽

Endang W Bachtiar

Keyword(s):

Orthodontic Treatment ◽

Treponema Denticola ◽

Tannerella Forsythia ◽

Chitosan Gel

Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia: the 'red complex', a prototype polybacterial pathogenic consortium in periodontitis

Periodontology 2000 ◽

10.1111/j.1600-0757.2005.00113.x ◽

2005 ◽

Vol 38 (1) ◽

pp. 72-122 ◽

Cited By ~ 504

Author(s):

Stanley C. Holt ◽

Jeffrey L. Ebersole

Keyword(s):

Treponema Denticola ◽

Treponema denticola, Porphyromonas gingivalis, and Tannerella forsythia induce cell death and release of endogenous danger signals

Archives of Oral Biology ◽

10.1016/j.archoralbio.2016.09.010 ◽

2017 ◽

Vol 73 ◽

pp. 72-78 ◽

Cited By ~ 15

Author(s):

Hye-Kyoung Jun ◽

Young-Jung Jung ◽

Bong-Kyu Choi

Keyword(s):

Cell Death ◽

Treponema Denticola ◽

Tannerella Forsythia ◽

Induce Cell Death ◽

Danger Signals

Potentiel pathogénique de Porphyromonas gingivalis, Treponema denticola et Tannerella forsythia, le complexe bactérien rouge associé à la parodontite

Pathologie Biologie ◽

10.1016/j.patbio.2006.07.045 ◽

2007 ◽

Vol 55 (3-4) ◽

pp. 154-162 ◽

Cited By ~ 36

Author(s):

C. Bodet ◽

F. Chandad ◽

D. Grenier

Keyword(s):

Treponema Denticola ◽

Identification of Bacteria Involved in Periodontal Disease Using Molecular Biology Techniques

Revista de Chimie ◽

10.37358/rc.17.10.5895 ◽

2017 ◽

Vol 68 (10) ◽

pp. 2407-2412

Author(s):

Ioana Martu ◽

Ancuta Goriuc ◽

Maria Alexandra Martu ◽

Ioana Vata ◽

Raluca Baciu ◽

...

Keyword(s):

Periodontal Disease ◽

Strong Association ◽

Aggressive Periodontitis ◽

Treponema Denticola ◽

Tissue Destruction ◽

Identification Of Bacteria ◽

Study Support ◽

Dental Plaques ◽

Tannerella Forsythensis

Periodontal disease is progressive and episodic in nature, with tissue destruction resulting from the host response to bacterial antigens and irritants.This study aimed to evaluate the genotype of a certain group of pathogen agents. In order to to this we used a total of 45 patients with PAG included in the epidemiological analyzes with a mean age of 34.7 Four samples were collected from the subgingival plaque from each patient, a total of 224 samples of dental plaques were investigated. All samples were tested for the presence of Tannerella forsythensis, Porphyromonas gingivalis and Treponema denticola. We found that patients had a large number of pockets colonized by Tannerella forsythensis gingivalis (88.6%) Porphyromonas gingivalis (59%) and Aggregatibacter actinomycetemcomitans (25%). The data of this study support the evidence Tannerella forsythensis as a periodontopatogen and confirmed a strong association between Porphyromonas gingivalis and Tannerella forsythensis in aggressive periodontitis and support previous findings that generalized aggressive periodontitis are associated with more complex microbiota.

Presence of archaea and selected bacteria in infected root canal systems

Canadian Journal of Microbiology ◽

10.1139/cjm-2017-0531 ◽

2018 ◽

Vol 64 (5) ◽

pp. 317-326 ◽

Cited By ~ 2

Author(s):

Ewa Brzezińska-Błaszczyk ◽

Elżbieta Pawłowska ◽

Tomasz Płoszaj ◽

Henryk Witas ◽

Urszula Godzik ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Root Canal ◽

Expression Profiles ◽

Rna Extraction ◽

Treponema Denticola ◽

Rrna Gene ◽

Pulp Tissue ◽

Infections of the root canal have polymicrobial etiology. The main group of microflora in the infected pulp is bacteria. There is limited data that archaea may be present in infected pulp tissue. The aim of this study was to check the prevalence of archaea in necrotic root canal samples obtained from patients with primary or post-treatment infection. The prevalence of selected bacteria species (Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Synergistes sp.) in necrotic samples was evaluated as well. Sixty-four samples from root canal were collected for DNA and RNA extraction. A PCR assay based on the 16S rRNA gene was used to determine the presence of archaea and selected bacteria. Of the 64 samples, 6 were analyzed by semiquantitative reverse transcription PCR to estimate expression profiles of 16S rRNA, and another 9 were selected for direct sequencing. Archaea were detected in 48.4% samples. Statistical analysis indicated a negative association in coexistence between archaea and Treponema denticola (P < 0.05; Pearson’s χ2 test). The main representative of the Archaea domain found in infected pulp tissue was Methanobrevibacter oralis. Archaea 16S rRNA gene expression was significantly lower than Synergistes sp., Porphyromonas gingivalis, and Tannerella forsythia (P < 0.05; Student’s t test). Thus, it can be hypothesized that archaea may participate in the endodontic microbial community.