Construction of an infectious clone of canine herpesvirus genome as a bacterial artificial chromosome

2006 ◽  
Vol 8 (4) ◽  
pp. 1054-1063 ◽  
Author(s):  
Jun Arii ◽  
Orkash Hushur ◽  
Kentaro Kato ◽  
Yasushi Kawaguchi ◽  
Yukinobu Tohya ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Infectious Clone ◽  
Artificial Chromosome ◽  
Canine Herpesvirus ◽  
Herpesvirus Genome

scholarly journals Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome

1997 ◽  
Vol 94 (26) ◽  
pp. 14759-14763 ◽  
Author(s):  
M. Messerle ◽  
I. Crnkovic ◽  
W. Hammerschmidt ◽  
H. Ziegler ◽  
U. H. Koszinowski
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Artificial Chromosome ◽  
Herpesvirus Genome

scholarly journals Systematic Excision of Vector Sequences from the BAC-Cloned Herpesvirus Genome during Virus Reconstitution

1999 ◽  
Vol 73 (8) ◽  
pp. 7056-7060 ◽  
Author(s):  
Markus Wagner ◽  
Stipan Jonjić ◽  
Ulrich H. Koszinowski ◽  
Martin Messerle
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Artificial Chromosome ◽  
Full Length ◽  
Wild Type ◽  
Mouse Cytomegalovirus ◽  
Vector Sequences ◽  
Viral Sequences ◽  
Herpesvirus Genome

ABSTRACT Recently the mouse cytomegalovirus (MCMV) genome was cloned as an infectious bacterial artificial chromosome (BAC) (M. Messerle, I. Crnković, W. Hammerschmidt, H. Ziegler, and U. H. Koszinowski, Proc. Natl. Acad. Sci. USA 94:14759–14763, 1997). The virus obtained from this construct is attenuated in vivo due to deletion of viral sequences and insertion of the BAC vector. We reconstituted the full-length MCMV genome and flanked the BAC vector with identical viral sequences. This new construct represents a versatile basis for construction of MCMV mutants since virus generated from the construct loses the bacterial sequences and acquires wild-type properties.

scholarly journals Development of a novel DNA-launched dengue virus type 2 infectious clone assembled in a bacterial artificial chromosome

2014 ◽  
Vol 180 ◽  
pp. 12-22 ◽  
Author(s):  
Jose A. Usme-Ciro ◽  
Jaime A. Lopera ◽  
Luis Enjuanes ◽  
Fernando Almazán ◽  
Juan C. Gallego-Gomez
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Dengue Virus ◽  
Virus Type ◽  
Infectious Clone ◽  
Artificial Chromosome ◽  
Dengue Virus Type 2

scholarly journals Construction and Manipulation of an Infectious Clone of the Bovine Herpesvirus 1 Genome Maintained as a Bacterial Artificial Chromosome

2002 ◽  
Vol 76 (13) ◽  
pp. 6660-6668 ◽  
Author(s):  
Timothy J. Mahony ◽  
Fiona M. McCarthy ◽  
Jennifer L. Gravel ◽  
Lani West ◽  
Peter L. Young
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Infectious Clone ◽  
Bovine Herpesvirus ◽  
Artificial Chromosome ◽  
Gene Encoding ◽  
Kinase Gene ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Herpesvirus 1

ABSTRACT The complete genome of bovine herpesvirus 1 (BoHV-1) strain V155 has been cloned as a bacterial artificial chromosome (BAC). Following electroporation into Escherichia coli strain DH10B, the BoHV-1 BAC was stably propagated over multiple generations of its host. BAC DNA recovered from DH10B cells and transfected into bovine cells produced a cytopathic effect which was indistinguishable from that of the parent virus. Analysis of the replication kinetics of the viral progeny indicated that insertion of the BAC vector into the thymidine kinase gene did not affect viral replication. Specific manipulation of the BAC was demonstrated by deleting the gene encoding glycoprotein E by homologous recombination in DH10B cells facilitated by GET recombination. These studies illustrate that the propagation and manipulation of herpesviruses in bacterial systems will allow for rapid and accurate characterization of BoHV-1 genes. In turn, this will allow for the full utilization of BoHV-1 as a vaccine vector.

scholarly journals Targeting CreERT2 expression to keratin 8-expressing murine simple epithelia using bacterial artificial chromosome transgenesis

2012 ◽  
Vol 21 (5) ◽  
pp. 1117-1123 ◽  
Author(s):  
Li Zhang ◽  
Boyu Zhang ◽  
Sang Jun Han ◽  
Amy N. Shore ◽  
Jeffrey M. Rosen ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Artificial Chromosome ◽  
Keratin 8

scholarly journals Revising the Chromosome-Specific Probes of White Hawk (Leucopternis albicollis)

Proceedings ◽  
2020 ◽  
Vol 76 (1) ◽  
pp. 1
Author(s):  
Ivanete Furo ◽  
Rafael Kretschmer ◽  
Jorge Pereira ◽  
Darren Griffin ◽  
Rebecca O’Connor ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Artificial Chromosome ◽  
Bird Of Prey ◽  
Inaccurate Data ◽  
Intrachromosomal Rearrangements

Leucopternis albicollis is a diurnal bird of prey with extensive karyotype reorganization. Chromosome-specific probes from this species have been used successfully to detect intrachromosomal rearrangements in different species of bird since 2010. However, some gaps were detected in this first set of probes. Here, we have obtained a new set of whole chromosome probes in order to improve the previous one; also, we have performed experiments using bacterial artificial chromosome (BAC) from chicken microchromosomes. Our results demonstrated that the microchromosomes were involved in fusion events. In addition, a new nomenclature has been proposed for the new set of probes and some inaccurate data were corrected.

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