Identification of an outer membrane protein of Salmonella enterica serovar Typhimurium as a potential vaccine candidate for Salmonellosis in mice

2013 ◽  
Vol 15 (5) ◽  
pp. 388-398 ◽  
Author(s):  
Youjun Yang ◽  
Cuixiang Wan ◽  
Hengyi Xu ◽  
Zoraida P. Aguilar ◽  
Qianglai Tan ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Vaccine Candidate ◽  
Potential Vaccine Candidate ◽  
Serovar Typhimurium ◽  
Potential Vaccine

scholarly journals Outer Membrane Protein STM3031 (Ail/OmpX-Like Protein) Plays a Key Role in the Ceftriaxone Resistance of Salmonella enterica Serovar Typhimurium

2009 ◽  
Vol 53 (8) ◽  
pp. 3248-3255 ◽  
Author(s):  
Wensi S. Hu ◽  
Jing-Fang Lin ◽  
Ying-Hsiu Lin ◽  
Hsin-Yu Chang
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Salmonella Enterica ◽  
Efflux Pump ◽  
Protein S ◽  
Mrna Levels ◽  
Expression Levels ◽  
Protein Levels ◽  
Serovar Typhimurium

ABSTRACT Previously, the putative outer membrane protein STM3031 has been correlated with ceftriaxone resistance in Salmonella enterica serovar Typhimurium. In this study, this protein was almost undetectable in the ceftriaxone-susceptible strain 01-4, but its levels were increased in 01-4 isogenic strains for which MICs were higher. The stm3031 gene deletion mutant, R200(Δstm3031), was generated and showed >64-fold lower ceftriaxone resistance than R200, supporting a key role for STM3031 in ceftriaxone resistance. To investigate which outer membrane protein(s) was associated with resistance, the outer membrane protein profiles of 01-4, R200, and R200(Δstm3031) were compared proteomically. Nine proteins were identified as altered. The expression levels of AcrA, TolC, STM3031, STM1530, VacJ, and Psd in R200 were increased; those of OmpC, OmpD, and OmpW were decreased. The expression levels of OmpD, OmpW, STM1530, VacJ, and Psd, but not those of OmpC, AcrA, and TolC, in R200(Δstm3031) were returned to the levels in strain 01-4. Furthermore, the genes' mRNA levels correlated with their protein levels when the three strains were compared. The detection of higher AcrB levels, linked to higher acrB, acrD, and acrF mRNA levels, in strain R200 than in strains 01-4 and R200(Δstm3031) suggests that AcrB, AcrD, and AcrF participate in ceftriaxone resistance. Taken together with the location of STM3031 in the outer membrane, these results suggest that STM3031 plays a key role in ceftriaxone resistance, probably by reducing permeability via a decreased porin OmpD level and enhancing export via increased AcrD efflux pump activity.

Sign in / Sign up

Export Citation Format

Share Document