Quantitative cell wall protein profiling of invasive and non-invasive Saccharomyces cerevisiae strains

AbstractThe gene SPI1, of Saccharomyces cerevisiae, encodes a cell wall protein that is induced in several stress conditions, particularly in the postdiauxic and stationary phases of growth. It has a paralogue, SED1, which shows some common features in expression regulation and in the null mutant phenotype. In this work we have identified homologues in other species of yeasts and filamentous fungi, and we have also elucidated some aspects of the origin of SPI1, by duplication and diversification of SED1. In terms of regulation, we have found that the expression in the post-diauxic phase is regulated by genes related to the PKA pathway and stress response (MSN2/4, YAK1, POP2, SOK2, PHD1, and PHO84) and by genes involved in the PKC pathway (WSC2, PKC1, and MPK1).

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Proteolytic processing of the Saccharomyces cerevisiae cell wall protein Scw4 regulates its activity and influences its covalent binding to glucan

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research ◽

10.1016/j.bbamcr.2016.12.009 ◽

2017 ◽

Vol 1864 (3) ◽

pp. 507-515 ◽

Cited By ~ 5

Author(s):

Antonija Grbavac ◽

Iva Čanak ◽

Igor Stuparević ◽

Renata Teparić ◽

Vladimir Mrša

Keyword(s):

Saccharomyces Cerevisiae ◽

Cell Wall ◽

Covalent Binding ◽

Cell Wall Protein ◽

Proteolytic Processing

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A polyphasic approach to highlight genotypic and phenotypic diversities of Lactobacillus helveticus strains isolated from dairy starter cultures and cheeses

Journal of Dairy Research ◽

10.1017/s0022029901005349 ◽

2002 ◽

Vol 69 (1) ◽

pp. 139-149 ◽

Cited By ~ 25

Author(s):

ANGIOLELLA LOMBARDI ◽

LUCIANA DAL MAISTRO ◽

PAOLA DE DEA ◽

MONICA GATTI ◽

GIORGIO GIRAFFA ◽

...

Keyword(s):

Cell Wall ◽

Phenotypic Diversity ◽

Protein Profile ◽

Genotypic Diversity ◽

Cell Wall Protein ◽

Starter Cultures ◽

Lactobacillus Helveticus ◽

Protein Profiling ◽

Rapd Pcr ◽

Dairy Starter Cultures

In the present work, 67 strains of Lactobacillus helveticus isolated from whey starter cultures and cheeses were identified and grouped by genotypic and phenotypic methods. Strains were identified by sugar fermentation pattern, by cell-wall protein profile, and by probe hybridisation. Phenotypic diversity was evaluated by a chemometric model taking into account biochemical characteristics (i.e. acidifying and peptidase activities) of technological interest. Genotypic diversity was evidenced by RAPD-PCR, which provided strain-specific patterns and revealed the occurrence of different strains. The RAPD-PCR profiles were clustered according to their similarities; the groups obtained, together with the cell-wall protein profiling and the chemometric information, could be sometimes correlated with the type of cheese and/or dairy niches used as sources of strains. A computerised analysis of genotypic and phenotypic information could be successfully applied for rapid and reliable differentiation and characterisation of Lb. helveticus isolates occurring in different dairy products.

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