Evaluation of the FilmArray Blood Culture Identification Panel compared to direct MALDI-TOF MS identification for rapid identification of pathogens

Using MALDI-TOF MS directly from blood culture bottles reduces the time required for pathogen identification, and the turnaround times for final identification have been compared with overnight incubation from solid media in previous studies. However, identification from a short incubation of agar plates has been increasingly accepted and successfully implemented in routine laboratories, but there is no data comparing direct MALDI-TOF MS with the short-term, incubated agar plates.

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Rapid identification of bacteria from positive blood culture bottles by MALDI-TOF MS following short-term incubation on solid media

Journal of Medical Microbiology ◽

10.1099/jmm.0.000168 ◽

2015 ◽

Vol 64 (11) ◽

pp. 1346-1352 ◽

Cited By ~ 35

Author(s):

Osman Altun ◽

Silvia Botero-Kleiven ◽

Sarah Carlsson ◽

Måns Ullberg ◽

Volkan Özenci

Keyword(s):

Blood Culture ◽

Positive Blood Culture ◽

Rapid Identification ◽

Short Term ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Solid Media ◽

Identification Of Bacteria ◽

Tof Ms

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Comparison of the Fully Automated FilmArray BCID Assay to a 4-Hour Culture Test Coupled to Mass Spectrometry for Day 0 Identification of Microorganisms in Positive Blood Cultures

BioMed Research International ◽

10.1155/2018/7013470 ◽

2018 ◽

Vol 2018 ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

Paul O. Verhoeven ◽

Cyrille H. Haddar ◽

Josselin Rigaill ◽

Nathalie Fonsale ◽

Anne Carricajo ◽

...

Keyword(s):

Blood Culture ◽

Positive Blood Culture ◽

Blood Stream Infection ◽

Blood Stream ◽

Bacterial Identification ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Negative Results ◽

Culture Identification ◽

Tof Ms

Rapid bacterial identification of positive blood culture is important for adapting the antimicrobial therapy in patients with blood stream infection. The aim of this study was to evaluate the performance of the multiplex FilmArray Blood Culture Identification (BCID) assay by comparison to an in-house protocol based on MALDI-TOF MS identification of microcolonies after a 4-hour culture, for identifying on the same day the microorganisms present in positive blood culture bottles. One hundred and fifty-three positive bottles from 123 patients were tested prospectively by the 3 techniques of bacterial identification: 11 bottles yielding negative results by the 3 tests were considered false positive (7.2%). The reference MALDI-TOF MS technique identified 134 monomicrobial (87.6%) and 8 double infections (5.2%), which resulted in a total of 150 microorganisms. Globally, 137 (91.3%) of these 150 pathogens were correctly identified by the fully automated multiplex FilmArray BCID system at the species or genus level on day of growth detection, versus 117 (78.8%) by MALDI-TOF MS identification on nascent microcolonies after a 4-hour culture (P < 0.01). By combining the two approaches, 140 (93.5%) of the positive bottles were identified successfully at day 0. These results confirm the excellent sensitivity of the FilmArray BCID assay, notably in case of multimicrobial infection. Due to the limited number of targets included into the test, it must be coupled to another identification strategy, as that presented in this study relying on MALDI-TOF MS identification of microcolonies obtained after a very short culture period.

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Evaluation of MALDI-TOF Mass Spectrometry and Sepsityper Kit™ for the Direct Identification of Organisms from Sterile Body Fluids in a Canadian Pediatric Hospital

Canadian Journal of Infectious Diseases and Medical Microbiology ◽

10.1155/2013/701093 ◽

2013 ◽

Vol 24 (4) ◽

pp. 191-194 ◽

Cited By ~ 23

Author(s):

Manal Tadros ◽

Astrid Petrich

Keyword(s):

Mass Spectrometry ◽

Blood Culture ◽

Software Tool ◽

Rapid Identification ◽

Correct Identification ◽

Direct Identification ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Flight Mass Spectrometry ◽

Tof Ms

Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) can be used to identify bacteria directly from positive blood and sterile fluid cultures. The authors evaluated a commercially available kit – the Sepsityper Kit (Bruker Daltonik, Germany) – and MALDI-TOF MS for the rapid identification of organisms from 80 flagged positive blood culture broths, of which 73 (91.2%) were blood culture specimens and seven (8.7%) were cerebrospinal fluid specimens, in comparison with conventional identification methods. Correct identification to the genus and species levels was obtained in 75 of 80 (93.8%) and 39 of 50 (78%) blood culture broths, respectively. Applying the blood culture analysis module, a newly developed software tool, improved the species identification of Gram-negative organisms from 94.7% to 100% and of Gram-positive organisms from 66.7% to 70%.MALDI-TOF MS is a promising tool for the direct identification of organisms cultured from sterile sites.

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The impact of blood culture identification by MALDI-TOF MS on the antimicrobial management of pediatric patients

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2018.05.021 ◽

2018 ◽

Vol 92 (3) ◽

pp. 220-225 ◽

Cited By ~ 6

Author(s):

Sejal Makvana Bhavsar ◽

Tanis C. Dingle ◽

Camille L. Hamula

Keyword(s):

Blood Culture ◽

Pediatric Patients ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Antimicrobial Management ◽

Culture Identification ◽

The Impact ◽

Tof Ms

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Comparative Analysis of PCR–Electrospray Ionization/Mass Spectrometry (MS) and MALDI-TOF/MS for the Identification of Bacteria and Yeast from Positive Blood Culture Bottles

Clinical Chemistry ◽

10.1373/clinchem.2011.161968 ◽

2011 ◽

Vol 57 (7) ◽

pp. 1057-1067 ◽

Cited By ~ 81

Author(s):

Erin J Kaleta ◽

Andrew E Clark ◽

Abdessalam Cherkaoui ◽

Vicki H Wysocki ◽

Elizabeth L Ingram ◽

...

Keyword(s):

Blood Culture ◽

Diagnostic Accuracy ◽

Bloodstream Infections ◽

Rapid Identification ◽

Blood Cultures ◽

Reference Standard ◽

Maldi Tof Ms ◽

Esi Ms ◽

Maldi Tof ◽

Tof Ms

BACKGROUND Emerging technologies for rapid identification of microbes demonstrate a shift from traditional biochemical and molecular testing algorithms toward methods using mass spectrometry (MS) for the semiquantitative analysis of microbial proteins and genetic elements. This study was performed to assess the diagnostic accuracy of 2 such technologies, PCR–electrospray ionization (ESI)/MS and MALDI-TOF/MS, with respect to phenotypic and biochemical profiling as a reference standard method. A positive challenge set of blood culture bottles was used to compare PCR-ESI/MS and MALDI-TOF/MS performance on a matched set of samples. METHODS We performed characterization of bloodstream infections from blood cultures using the Ibis T5000 PCR-ESI/MS and the Bruker MALDI Biotyper 2.0 (MALDI-TOF/MS) platforms for microbial identification. Diagnostic accuracy was determined by independent comparison of each method to phenotypic and biochemical characterization with Vitek2 analysis as the reference standard identification. RESULTS The diagnostic accuracy, represented as positive agreement, at the genus level was 0.965 (0.930–0.984) for PCR-ESI/MS and 0.969 (0.935–0.987) for MALDI-TOF/MS, and at the species level was 0.952 (0.912–0.974) with PCR-ESI/MS and 0.943 (0.902–0.968) for MALDI-TOF/MS. No statistically significant difference was found between PCR-ESI/MS and MALDI-TOF/MS in the ability to rapidly identify microorganisms isolated from blood culture. CONCLUSIONS Our results demonstrate that PCR-ESI/MS and MALDI-TOF/MS are equivalent in their ability to characterize bloodstream infections with respect to the reference standard, and highlight key differences in the methods that allow for each method to have a unique niche as a tool for rapid identification of microbes in blood cultures.

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