scholarly journals Development of a 128-channel multi-water-sampling system for underwater platforms and its application to chemical and biological monitoring

2013 ◽  
Vol 8 ◽  
pp. 75-90 ◽  
Author(s):  
Kei Okamura ◽  
Takuroh Noguchi ◽  
Mayumi Hatta ◽  
Michinari Sunamura ◽  
Takahiko Suzue ◽  
...  
1993 ◽  
Vol 13 (1) ◽  
pp. 115-120 ◽  
Author(s):  
Barry S. Mines ◽  
John L. Davidson ◽  
David Bloomquist ◽  
Thomas B. Stauffer

2019 ◽  
Vol 22 (3) ◽  
pp. 126-134 ◽  
Author(s):  
Daeho Kang ◽  
Junho Jeon ◽  
Moonhwan Song ◽  
Jinsung Ra

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4290 ◽  
Author(s):  
Craig Powers ◽  
Regina Hanlon ◽  
David G. Schmale III

Microorganisms are ubiquitous in freshwater aquatic environments, but little is known about their abundance, diversity, and transport. We designed and deployed a remote-operated water-sampling system onboard an unmanned surface vehicle (USV, a remote-controlled boat) to collect and characterize microbes in a freshwater lake in Virginia, USA. The USV collected water samples simultaneously at 5 and 50 cm below the surface of the water at three separate locations over three days in October, 2016. These samples were plated on a non-selective medium (TSA) and on a medium selective for the genusPseudomonas(KBC) to estimate concentrations of culturable bacteria in the lake. Mean concentrations ranged from 134 to 407 CFU/mL for microbes cultured on TSA, and from 2 to 8 CFU/mL for microbes cultured on KBC. There was a significant difference in the concentration of microbes cultured on KBC across three sampling locations in the lake (P= 0.027), suggesting an uneven distribution ofPseudomonasacross the locations sampled. There was also a significant difference in concentrations of microbes cultured on TSA across the three sampling days (P= 0.038), demonstrating daily fluctuations in concentrations of culturable bacteria. There was no significant difference in concentrations of microbes cultured on TSA (P= 0.707) and KBC (P= 0.641) across the two depths sampled, suggesting microorganisms were well-mixed between 5 and 50 cm below the surface of the water. About 1 percent (7/720) of the colonies recovered across all four sampling missions were ice nucleation active (ice+) at temperatures warmer than −10 °C. Our work extends traditional manned observations of aquatic environments to unmanned systems, and highlights the potential for USVs to understand the distribution and diversity of microbes within and above freshwater aquatic environments.


2020 ◽  
Vol 64 (9) ◽  
pp. 982-992
Author(s):  
Tobias Weiss ◽  
Dietmar Breuer ◽  
Daniel Bury ◽  
Claudia Friedrich ◽  
Silke Werner ◽  
...  

Abstract Exposure to the bicyclic aromatic hydrocarbon naphthalene occurs in most cases along with other polycyclic aromatic hydrocarbons. Here we report from an investigation of 63 healthy, non-smoking male employees in the abrasives industry where naphthalene is the only relevant chemical exposure. Exposure assessment was performed using a combination of Air and Biological Monitoring over nearly a whole working week (Mo.–Th.). Air measurements were carried out during the shift on Thursday with the GGP mini-sampling system, combining particle and vapour sampling at low flow rates. In urine spot samples, the metabolites 1- and 2-naphthol were measured Mo.–Th. pre- and post-shift (for the reference group only Mo. pre- and Th. post-shift). With regard to naphthalene concentrations measured in air and concentrations of its metabolites (1- and 2-naphthol) in urine, study participants could be divided into a high and a low exposure group, and a reference group. The naphthalene concentration in air was in the range of 0.1–11.6 mg m−3, and naphthol concentrations (sum of 1- and 2-naphthol) in post-shift urine were in the range of <1 to 10 127 µg l−1. Naphthalene concentrations in air and naphthol concentrations in urine were closely correlated, indicating mainly airborne exposure at the investigated workplaces. As expected from toxicokinetic data, internal body burden increased slightly during a working week and did not completely decline over a work-free weekend to background concentrations observed in occupationally not exposed persons. Taking into account the observed increase in pre- and post-shift values during the working week, urine sampling for Biological Monitoring at workplaces should be carried out after several preceding shifts. Our data allow the derivation of biological limit values for the sum of 1- and 2-naphthol in urine corresponding to occupational exposure limits for naphthalene in air.


Water ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 157 ◽  
Author(s):  
James Benson ◽  
Regina Hanlon ◽  
Teresa Seifried ◽  
Philipp Baloh ◽  
Craig Powers ◽  
...  

New tools and technology are needed to study microorganisms in freshwater environments. Little is known about spatial distribution and ice nucleation activity (INA) of microorganisms in freshwater lakes. We developed a system to collect water samples from the surface of lakes using a 3D-printed sampling device tethered to a drone (DOWSE, DrOne Water Sampling SystEm). The DOWSE was used to collect surface water samples at different distances from the shore (1, 25, and 50 m) at eight different freshwater lakes in Austria in June 2018. Water samples were filtered, and microorganisms were cultured on two different media types, TSA (a general growth medium) and KBC (a medium semi-selective for bacteria in the genus Pseudomonas). Mean concentrations (colony forming units per mL, or CFU/mL) of bacteria cultured on TSA ranged from 19,800 (Wörthersee) to 210,500 (Gosaulacke) CFU/mL, and mean concentrations of bacteria cultured on KBC ranged from 2590 (Ossiachersee) to 11,000 (Vorderer Gosausee) CFU/mL. There was no significant difference in sampling distance from the shore for concentrations of microbes cultured on TSA (p = 0.28). A wireless bathymetry sensor was tethered to the drone to map temperature and depth across the sampling domain of each of the lakes. At the 50 m distance from the shore, temperature ranged from 17 (Hinterer Gosausee, and Gosaulacke) to 26 °C (Wörthersee), and depth ranged from 2.8 (Gosaulacke) to 11.1 m (Grundlsee). Contour maps of concentrations of culturable bacteria across the drone sampling domain revealed areas of high concentrations (hot spots) in some of the lakes. The percentage of ice-nucleation active (ice+) bacteria cultured on KBC ranged from 0% (0/64) (Wörthersee) to 58% (42/72) (Vorderer Gosausee), with a mean of 28% (153/544) for the entire sample set. Future work aims to elucidate the structure and function of entire microbial assemblages within and among the Austrian lakes.


2014 ◽  
Vol 11 (5) ◽  
pp. 5115-5142 ◽  
Author(s):  
C. Comina ◽  
M. Lasagna ◽  
D. A. De Luca ◽  
L. Sambuelli

Abstract. To improve water management design, particularly in irrigation areas, it is important to evaluate the baseline state of the water resources, including canal discharge. Discharge measurements, using salt dilution gauging, are a traditional and well-documented technique. The complete mixing of salt used for dilution gauging is required for reliable measurements; this condition is difficult to test or verify and, if not fulfilled, is the largest source of uncertainty in the discharge calculation. In this paper, a geophysical technique (FERT, Fast Electrical Resistivity Tomography) is proposed for imaging the distribution of the salt plume used for dilution gauging at every point along a sampling cross-section. In this way, it is possible to check whether complete mixing has occurred. If the mixing is not complete, the image created by FERT can also provide guidance for selecting water-sampling locations in the sampling cross-section. A water multi-sampling system prototype for the simultaneous sampling of canal water at different points within the cross-section, aimed to potentially take into account concentration variability, is also proposed and tested. Preliminary results of a single test with salt dilution gauging and FERT in a real case are reported. The results show that imaging the passage of the salt plume is possible by means of geophysical controls and that this can potentially help in the selection of water sampling points.


Water ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 604 ◽  
Author(s):  
Cengiz Koparan ◽  
Ali Koc ◽  
Charles Privette ◽  
Calvin Sawyer

The objective of this research was to conduct in situ measurements of electrical conductivity (EC), pH, dissolved oxygen (DO), and temperature, and collect water samples simultaneously at different depths using an unmanned aerial vehicle (UAV). The UAV system consists of a hexacopter, water sampling cartridges (WSC), and a sensor node. Payload capacity and endurance of the UAV were determined using an indoor test station. The UAV was able to produce 106 N of thrust for 10 min with 6.3 kg of total takeoff weight. The thrust-to-weight ratio of the UAV was 2.5 at 50% throttle. The decision for activating the water sampling cartridges and sensor node was made autonomously from an onboard microcontroller. System functions were verified at 0.5 m and 3.0 m depths in 6 locations over a 1.1 ha agricultural pond. Average measurements of EC, pH, DO, and temperature at 0.5 m depth were 42 µS/cm, 5.6, 8.2 mg/L, and 31 °C, while the measurements at 3 m depth were 80 µS/cm, 5.3, 5.34 mg/L, and 24 °C, respectively. The UAV-assisted autonomous water sampling system (UASS) successfully activated the WSC at each sampling location. The UASS would reduce the duration of water quality assessment and help practitioners and researchers to conduct observations with lower operational costs. The developed system would be useful for sampling and monitoring of water reservoirs, lakes, rivers, and ponds periodically or after natural disasters.


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