A codon-optimized endoprotease Endo-Pro-Aspergillus niger: Over expression and high-density fermentation in Pichia pastoris

2014 ◽  
Vol 104 ◽  
pp. 64-69
Author(s):  
Chao Kang ◽  
Xiao-Wei Yu ◽  
Yan Xu
Keyword(s):  
Aspergillus Niger ◽  
Pichia Pastoris ◽  
High Density ◽  
Over Expression

Over expression of anti-MUC1 single-domain antibody fragments in the yeast Pichia pastoris

2006 ◽  
Vol 43 (5) ◽  
pp. 426-435 ◽  
Author(s):  
Fatemeh Rahbarizadeh ◽  
Mohammad J. Rasaee ◽  
Mehdi Forouzandeh ◽  
Abdol-Amir Allameh
Keyword(s):  
Pichia Pastoris ◽  
Antibody Fragments ◽  
Single Domain ◽  
Single Domain Antibody ◽  
Yeast Pichia Pastoris ◽  
Over Expression ◽  
Domain Antibody

Synthesis of recombinant high density lipoprotein with apolipoprotein A-I and apolipoprotein A-V

2011 ◽  
Vol 392 (5) ◽  
Author(s):  
Xinbo Zhang ◽  
Baosheng Chen
Keyword(s):  
High Density Lipoprotein ◽  
Low Density Lipoprotein ◽  
Atherosclerotic Lesion ◽  
Density Lipoprotein ◽  
High Density ◽  
Over Expression ◽  
Apolipoprotein A ◽  
Atherosclerotic Lesion Development

Abstract It has been shown that apolipoprotein A-V (apoA-V) over-expression significantly lowers plasma triglyceride levels and decreases atherosclerotic lesion development. To assess the feasibility of recombinant high density lipoprotein (rHDL) reconstituted with apoA-V and apolipoprotein A-I (apoA-I) as a therapeutic agent for hyperlipidemic disorder and atherosclerosis, a series of rHDL were synthesized in vitro with various mass ratios of recombinant apoA-I and apoA-V. It is interesting to find that apoA-V of rHDL had no effect on lipoprotein lipase (LPL) activation in vitro and very low density lipoprotein (VLDL) clearance in HepG2 cells and in vivo. By contrast, LPL activation and VLDL clearance were inhibited by the addition of apoA-V to rHDL. Furthermore, the apoA-V of rHDL could not redistribute from rHDL to VLDL after incubation at 37°C for 30 min. These findings suggest that an increase of apoA-V in rHDL could not play a role in VLDL clearance in vitro and in vivo, which could, at least in part, attribute to the lost redistribution of apoA-V from rHDL to VLDL and LPL binding ability of apoA-V in rHDL. The therapeutic application of rHDL reconstituted with apoA-V and apoA-I might need the construction of rHDL from which apoA-V could freely redistribute to VLDL.

Heterologous Expression of Trametes versicolor Laccase in Pichia pastoris and Aspergillus niger

2006 ◽  
Vol 129 (1-3) ◽  
pp. 195-214 ◽  
Author(s):  
Christina Bohlin ◽  
Leif J. Jönsson ◽  
Robyn Roth ◽  
Willem H. van Zyl
Keyword(s):  
Aspergillus Niger ◽  
Pichia Pastoris ◽  
Heterologous Expression ◽  
Trametes Versicolor

Synthesis of Isomalto-Oligosaccharides by Pichia pastoris Displaying the Aspergillus niger α-Glucosidase

2017 ◽  
Vol 65 (43) ◽  
pp. 9468-9474 ◽  
Author(s):  
Nannan Zhao ◽  
Yanshan Xu ◽  
Kuang Wang ◽  
Suiping Zheng
Keyword(s):  
Aspergillus Niger ◽  
Pichia Pastoris

scholarly journals The transcriptomic fingerprint of glucoamylase over-expression in Aspergillus niger

BMC Genomics ◽  
2012 ◽  
Vol 13 (1) ◽  
pp. 701 ◽  
Author(s):  
Min Jin Kwon ◽  
Thomas R Jørgensen ◽  
Benjamin M Nitsche ◽  
Mark Arentshorst ◽  
Joohae Park ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Over Expression
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