scholarly journals MAPKAP Kinase-2 Is a Cell Cycle Checkpoint Kinase that Regulates the G2/M Transition and S Phase Progression in Response to UV Irradiation

2005 ◽  
Vol 17 (1) ◽  
pp. 37-48 ◽  
Author(s):  
Isaac A. Manke ◽  
Anhco Nguyen ◽  
Daniel Lim ◽  
Mary Q. Stewart ◽  
Andrew E.H. Elia ◽  
...  
2004 ◽  
Vol 279 (16) ◽  
pp. 16433-16440 ◽  
Author(s):  
D. Alwyn Dart ◽  
Kate E. Adams ◽  
Ildem Akerman ◽  
Nicholas D. Lakin

1997 ◽  
Vol 17 (6) ◽  
pp. 3103-3115 ◽  
Author(s):  
M Uchiyama ◽  
I Galli ◽  
D J Griffiths ◽  
T S Wang

A semipermissive growth condition was defined for a Schizosaccharomyces pombe strain carrying a thermosensitive allele of DNA polymerase delta (pol delta ts03). Under this condition, DNA polymerase delta is semidisabled and causes a delay in S-phase progression. Using a genetic strategy, we have isolated a panel of mutants that enter premature mitosis when DNA replication is incomplete but which are not defective for arrest in G2/M following DNA damage. We characterized the aya14 mutant, which enters premature mitosis when S phase is arrested by genetic or chemical means. However, this mutant is sensitive to neither UV nor gamma irradiation. Two genomic clones, rad26+ and cds1+, were found to suppress the hydroxyurea sensitivity of the aya14 mutant. Genetic analysis indicates that aya14 is a novel allele of the cell cycle checkpoint gene rad26+, which we have named rad26.a14. cds1+ is a suppressor which suppresses the S-phase feedback control defect of rad26.a14 when S phase is inhibited by either hydroxyurea or cdc22, but it does not suppress the defect when S phase is arrested by a mutant DNA polymerase. Analyses of rad26.a14 in a variety of cdc mutant backgrounds indicate that strains containing rad26.a14 bypass S-phase arrest but not G1 or late S/G2 arrest. A model of how Rad26 monitors S-phase progression to maintain the dependency of cell cycle events and coordinates with other rad/hus checkpoint gene products in responding to radiation damage is proposed.


EMBO Reports ◽  
2009 ◽  
Vol 10 (9) ◽  
pp. 1029-1035 ◽  
Author(s):  
Nianxiang Zhang ◽  
Ramandeep Kaur ◽  
Shamima Akhter ◽  
Randy J Legerski

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