Immobilizing salicyl fluorone and hexadecyl pyridine bromide as chemosensors on a glutaraldehyde-coated eggshell membrane to design a “turn-off” fluorescent sensor for copper detection.
Many biomacromolecules are known to cluster in microdomains with specific
subcellular localization. In the case of enzymes, this clustering greatly defines their biological
functions. Nitroreductases are enzymes capable of reducing nitro groups to amines and play a
role in detoxification and pro-drug activation. Although nitroreductase activity has been detected
in mammalian cells, the subcellular localization of this activity remains incompletely
characterized. Here, we report a fluorescent probe that enables super-resolved imaging of pools
of nitroreductase activity within mitochondria. This probe is activated sequentially by
nitroreductases and light to give a photo-crosslinked adduct of active enzymes. In combination
with a general photoactivatable marker of mitochondria, we performed two-color, threedimensional, single-molecule localization microscopy. These experiments allowed us to image
the sub-mitochondrial organization of microdomains of nitroreductase activity.<br>
Many biomacromolecules are known to cluster in microdomains with specific
subcellular localization. In the case of enzymes, this clustering greatly defines their biological
functions. Nitroreductases are enzymes capable of reducing nitro groups to amines and play a
role in detoxification and pro-drug activation. Although nitroreductase activity has been detected
in mammalian cells, the subcellular localization of this activity remains incompletely
characterized. Here, we report a fluorescent probe that enables super-resolved imaging of pools
of nitroreductase activity within mitochondria. This probe is activated sequentially by
nitroreductases and light to give a photo-crosslinked adduct of active enzymes. In combination
with a general photoactivatable marker of mitochondria, we performed two-color, threedimensional, single-molecule localization microscopy. These experiments allowed us to image
the sub-mitochondrial organization of microdomains of nitroreductase activity.<br>
Real-time sensing of Chemical Warfare Agents (CWAs) is today a crucial topic to
prevent the lethal effects of a terroristic chemical attack. For this reason, the development of
efficient, selective, sensitive and reversible sensoristic devices, able to detect by optical response
ppm levels of these compounds, is strongly required. Here, the synthesis of a new fluorescent
sensor based on a salen-uranyl scaffold, functionalized with two bodipy moieties, and
its application for the detection of sub-ppm levels of CWAs is reported. Detection properties
were evaluated by fluorescence measurements and selectivity tests demonstrated the strong
affinity for CWAs.
Expedite Fluorescent Sensor Prototype for Hydrogen Peroxide Detection with Long-Life Test Substrates