Two-photon excited photoluminescence lifetime imaging studies on individual gelatin-coated gold nanorods

2021 ◽  
pp. 130785
Author(s):  
Ana-Maria Craciun ◽  
Laurentiu Susu ◽  
Monica Baia
Keyword(s):  
Gold Nanorods ◽  
Imaging Studies ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Photoluminescence Lifetime

Visualization of Cutaneous Distribution of Minocycline of a Topical Gel in Human Facial Skin with Two-Photon Excited Fluorescence Lifetime Imaging Microscopy (FLIM) and Phasor Analysis

2017 ◽  
Vol 1 ◽  
pp. s57
Author(s):  
Sinyoung Jeong ◽  
Maiko Hermsmeier ◽  
Sam Osseiran ◽  
Akira Yamamoto ◽  
Usha Nagavarapu ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Facial Skin ◽  
Topical Gel ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Phasor Analysis

Abstract Not AvailableStudy supported by BioPharmX.

Dynamic Monitoring of Phase-Separated Biomolecular Condensates by Photoluminescence Lifetime Imaging

2021 ◽  
Vol 93 (5) ◽  
pp. 2988-2995
Author(s):  
Zihe Yan ◽  
Jianfeng Xue ◽  
Min Zhou ◽  
Jinyu Wang ◽  
Yanxin Zhang ◽  
...  
Keyword(s):  
Dynamic Monitoring ◽  
Lifetime Imaging ◽  
Photoluminescence Lifetime

Parallel Nanometric 3D Tracking of Intracellular Gold Nanorods Using Multifocal Two-Photon Microscopy

Nano Letters ◽  
2013 ◽  
Vol 13 (3) ◽  
pp. 980-986 ◽  
Author(s):  
Bram van den Broek ◽  
Brian Ashcroft ◽  
Tjerk H. Oosterkamp ◽  
John van Noort
Keyword(s):  
Gold Nanorods ◽  
3D Tracking ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Sperm metabolism is altered during storage by female insects: evidence from two-photon autofluorescence lifetime measurements in bedbugs

2015 ◽  
Vol 12 (110) ◽  
pp. 20150609 ◽  
Author(s):  
Klaus Reinhardt ◽  
Hans Georg Breunig ◽  
Aisada Uchugonova ◽  
Karsten König
Keyword(s):  
Fluorescence Lifetime ◽  
Sperm Cells ◽  
Fluorescence Lifetime Imaging ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Photon Excitation ◽  
The Common ◽  
The Mean ◽  
The Difference ◽  
Male Genotype

We explore the possibility of characterizing sperm cells without the need to stain them using spectral and fluorescence lifetime analyses after multi-photon excitation in an insect model. The autofluorescence emission spectrum of sperm of the common bedbug, Cimex lectularius , was consistent with the presence of flavins and NAD(P)H. The mean fluorescence lifetimes showed smaller variation in sperm extracted from the male (tau m, τ m = 1.54–1.84 ns) than in that extracted from the female sperm storage organ (tau m, τ m = 1.26–2.00 ns). The fluorescence lifetime histograms revealed four peaks. These peaks (0.18, 0.92, 2.50 and 3.80 ns) suggest the presence of NAD(P)H and flavins and show that sperm metabolism can be characterized using fluorescence lifetime imaging. The difference in fluorescence lifetime variation between the sexes is consistent with the notion that female animals alter the metabolism of sperm cells during storage. It is not consistent, however, with the idea that sperm metabolism represents a sexually selected character that provides females with information about the male genotype.

Gold nanorods as dual photo-sensitizing and imaging agents for two-photon photodynamic therapy

Nanoscale ◽  
2012 ◽  
Vol 4 (24) ◽  
pp. 7712 ◽  
Author(s):  
Tingting Zhao ◽  
Xiaoqin Shen ◽  
Lin Li ◽  
Zhenping Guan ◽  
Nengyue Gao ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Gold Nanorods ◽  
Imaging Agents ◽  
Two Photon
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